Improved cigarette smoke in-vitro contamination method

A technology of cigarette smoke and gas, which is applied in the field of in vitro drug exposure of cigarette smoke, and can solve problems such as difficulty in controlling the concentration of cigarette smoke
CN101671733AInactive Publication Date: 2010-03-17ZHENGZHOU TOBACCO RES INST OF CNTC
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
ZHENGZHOU TOBACCO RES INST OF CNTC
Publication Date
2010-03-17
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to an improved cigarette smoke in-vitro contamination method. The biological effect of the cigarette smoke is evaluated by mixing the gaseous phase part (GVP) and the particle phase part (TPM) of the cigarette smoke to carry out the in-vitro contamination method. Specifically, the improved cigarette smoke in-vitro contamination method comprises the following steps: a. collection of the particle phase part and the gaseous phase part of smoke; b. mixing of the particle phase part and the gaseous phase part; and c, in-vitro contamination: diluting TPM+GVP with a proportion of 1:1 into different concentration gradients, and carrying out a cytotoxicity test, a micronucleus test and an Ames test by in-vitro contamination method. The invention has the advantages that the gaseous phase part of cigarette smoke is collected by an absorption bottle; the in-vitro contamination way of combining GVP and TPM can comprehensively and truly reflect the biological effect of the cigarette smoke; based on the conventional TPM contamination way, the contamination of the gaseous phase part of cigarette smoke is added, so that the in-vitro contamination method is more close to the actual cigarette smoke exposure condition; besides, the contamination concentration is easy to control and the operation is simple.
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Description

technical field

[0001] The present invention relates to an in vitro poisoning method of cigarette smoke, that is, adopting the method of combining cigarette smoke gas phase part (GVP) and particle phase part (TPM) for in vitro poisoning, so that the test object (cell or bacteria) The exposure environment is closer to the actual cigarette smoke exposure. Background technique

[0002] In terms of toxicological evaluation of cigarette additives, some foreign research institutions and companies have carried out a lot of research work. Clements et al conducted a study on the in vitro toxicological evaluation of cigarette additives. The research focused on the research design of the in vitro toxicological evaluation of cigarette additives. According to the standard method of genetic toxicology testing, bacterial mutation-Ames test, mammalian cell mutation-mouse lymphocytes Tumor analysis and chromosome structure variation analysis of cultured human cells or Chinese hamster cells,...

Claims

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