Antibody conjugate and method thereof

Abstract

The invention provides an antibody conjugate and a method thereof, and the antibody conjugate comprises: (1) an activation buffer which is a 0.05 M MES buffer with a pH of 5.0-6.0; (2) a reaction buffer which is a 0.04 M phosphate buffer with a pH of 7.0-8.5; (3) a stopping solution which comprises a 0.04 M phosphate buffer with a pH of 7.0-8.5, and 0.04 M Gly; (4) a blocking solution which comprises a 0.04 M phosphate buffer with a pH of 7.0-8.5, and a BSA solution with a mass fraction of 0.01%-1%; (5) a preservation solution which is a 0.02 M Tris buffer with a pH of 7.0-7.8; (6) a reaction buffer which comprises a 0.02 M phosphate buffer with a pH of 7.0-7.5, 0.15 M sodium chloride, and sodium dodecyl benzene sulfonate with a mass fraction of 0.005%-0.5%; (7) latex which is latex particles with carboxyls.

Description

technical field

[0001] The present invention relates to an antibody conjugate and its method, in particular to an antibody conjugate with high conjugation efficiency and its method. Background technique

[0002] The current antibody coupling technology is relatively immature, the antibody coupling efficiency is relatively low during the coupling process, and the size of the coupled latex particles is not suitable. There are mainly the following disadvantages: 1. The description of coupling conditions is not clear enough, it is difficult to find suitable coupling conditions; 2. There are many coupling methods, and it is difficult to find suitable coupling methods; 3. The activation of latex particles The requirements of the group are relatively broad, which makes it difficult to find a suitable group for coupling; 4. The description of various buffers in the coupling process is not clear enough, which makes it difficult to grasp the concentration of various ions in the coupli...

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