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Application of ruthenium complex modified nano-selenium in promoting osteogenic differentiation of mesenchymal stem cells

A ruthenium complex and mesenchymal stem cell technology, applied in the application field of ruthenium complex modified nano-selenium in promoting osteogenic differentiation of mesenchymal stem cells, to achieve the effect of increasing drug intake, simple product system, and inhibiting adipogenic differentiation

Inactive Publication Date: 2016-04-13
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, there is no report about the use of nano-selenium to promote osteogenic differentiation of mesenchymal stem cells

Method used

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  • Application of ruthenium complex modified nano-selenium in promoting osteogenic differentiation of mesenchymal stem cells
  • Application of ruthenium complex modified nano-selenium in promoting osteogenic differentiation of mesenchymal stem cells
  • Application of ruthenium complex modified nano-selenium in promoting osteogenic differentiation of mesenchymal stem cells

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Experimental program
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Effect test

Embodiment 1

[0036] Embodiment 1: the preparation of the nano selenium (Cit-RuSe) of bipyridyl ruthenium complex-citric acid modification

[0037] [Ru(bpy) 2 (dhipH 3 )] (ClO 4 ) 2 Preparation of: cis-[Ru(bpy) 2 Cl 2 ]·2H 2 O (Guangzhou Dongzheng Huabo Instrument Co., Ltd.) (0.156g, 0.3mmol) and 3,4-dihydroxy-imidazo[4,5-f][1,10]-phenanthroline (dhipH 3 ) (Guangzhou Dongzheng Huabo Instrument Co., Ltd.) (0.098g, 0.3mmol) was dissolved in 10mL of ethylene glycol, and heated at 120°C for 6h under the protection of argon. The solution gradually turned bright red. After cooling to room temperature, add saturated NaClO dropwise while stirring 4 , immediately produced a large amount of red precipitate. After stirring and standing still, the solution was suction-filtered, and the crude product was washed and dried, and then separated with a neutral alumina (200 mesh) column. The eluent is a mixed solvent of V (toluene): V (acetonitrile) = 1:4, the main red band components are collected,...

Embodiment 2

[0046] Embodiment 2: The experiment that bipyridyl ruthenium complex-citric acid modified nano-selenium (Cit-RuSe) promotes osteogenic differentiation of stem cells in vitro

[0047] Isolation and culture of human umbilical cord mesenchymal stem cells (HUMSCs): According to the hospital's regulations and with the consent of the family members, the umbilical cord tissues of normal full-term healthy infants were taken from the operating table and soaked in DMEM. Take out the umbilical cord in the ultra-clean workbench, fully wash the residual blood on the umbilical cord with D-Hanks’ balance solution, remove the umbilical vein, umbilical artery and umbilical cord adventitia, peel off the Wharton glue, and cut it into pieces of about 1mm 3 organization block. Move the tissue block into collagenase II (1g / L), add a little DMEM, and digest in a constant temperature shaker at 37°C until Wharton's glue is completely digested. Aspirate the digestive solution containing cells into a s...

Embodiment 3

[0051] Embodiment 3: The experiment that bipyridyl ruthenium complex-citric acid modified nano-selenium (Cit-RuSe) inhibits stem cell adipogenic differentiation in vitro

[0052] Human umbilical cord mesenchymal stem cells (5×10 per well 5 cells) were seeded in 24-well tissue culture plates in 5% CO 2 , 37°C incubator for 3 days. Remove the basal medium and replace it with an induction solution containing adipogenic inducing components (i.e. adipogenic inducing agent AS, DMEM (L)+10% FBS+10 μmol / L dexamethasone+200 μmol / L indomethacin+0.5mmol / L3-isobutyl-1-methylxanthine (IBMX)+10mg / L insulin), and add respectively the bipyridyl ruthenium complex modified nano-selenium (final concentration is 5 μ g / mL) or NaF ( The final concentration is 1mmol / L), induced for 9 and 18 days. Adding the ruthenium complex of the present invention to modify nano-selenium in the medium is the experimental group, and NaF is the positive control group. Fat droplets within stem cell-differentiate...

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Abstract

The invention belongs to the technical field of promotion of mesenchymal stem cell in-vitro osteoblast differentiation, and discloses an application of ruthenium complex modified nano selenium in promotion of mesenchymal stem cell in-vitro osteoblast differentiation. The invention provides the application of the ruthenium complex modified nano selenium in promotion of mesenchymal stem cell in-vitro osteoblast differentiation. The nano selenium can promote the mesenchymal stem cell in-vitro osteoblast differentiation of human umbilical cord, the differentiation balance is exsited between the mesenchymal stem cell ossification differentiation and esterification differentiation, so that the nano selenium has the effect for inhibiting esterification differentiation of mesenchymal stem cells, and can effectively labeling the mesenchymal stem cell. According to the invention, a ruthenium complex is used for modifying nano selenium, other auxiliary reagents are required for adding during a preparation process, the products system is simple, and the product can be directly preserved and used. The employed modification agent can greatly reduce the cytotoxicity of the ruthenium complex modified nano selenium, usage amount of the medicine for cells can be increased, external discharge is reduced, and the medicine in the cell can keep as a high level.

Description

technical field [0001] The invention belongs to the technical field of promoting osteogenic differentiation of mesenchymal stem cells, and in particular relates to the application of a ruthenium complex modified nano-selenium in promoting the osteogenic differentiation of mesenchymal stem cells. Background technique [0002] With the extension of human life span and the replacement of automobiles, bone diseases such as osteoporosis and osteoporotic fractures have become serious global public health problems. Bone disease is a manifestation of an imbalance between bone formation and bone resorption in bone metabolism. The general process of bone remodeling is: bone resorption → bone formation → bone rest → bone resorption, such a cycle goes back and forth. Osteoblasts (Osteoblast, OB) and osteoclasts (Osteoclast, OC) are two main cells that maintain bone shape and size in bone remodeling. OB is derived from mesenchymal stem cells, which can promote bone formation, secrete a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/0775C12N5/0735
Inventor 刘杰郑楚萍刘亚楠
Owner JINAN UNIVERSITY
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