Application of GDF11 (Growth Differentiation Factor 11) in preparing product for treating obesity and product for treating obesity

A technology of GDF11, 1.GDF11, which is applied in the field of obesity treatment products, can solve the problems of many adverse reactions, limited use, and insignificant curative effect, and achieve the effect of promoting fat catabolism, inhibiting obesity, and less side effects

Pending Publication Date: 2018-10-09
JINAN UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] The second object of the present invention is to provide a product for the treatment of obesity, so as to alleviate the technical problems of insignificant curative effect or many adverse reactions of weight loss drugs in the prior art, and limited clinical use

Method used

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  • Application of GDF11 (Growth Differentiation Factor 11) in preparing product for treating obesity and product for treating obesity
  • Application of GDF11 (Growth Differentiation Factor 11) in preparing product for treating obesity and product for treating obesity
  • Application of GDF11 (Growth Differentiation Factor 11) in preparing product for treating obesity and product for treating obesity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0087] Example 1 Effect of GDF11 on Adipogenic Differentiation of Adipose Stem Cells

[0088] 1. Effect of GDF11 on lipid droplet formation in adipose stem cells

[0089] In order to explore the effect of GDF11 on the adipogenic differentiation of adipose stem cells, this embodiment uses the adipogenic induction medium (high sugar DMEM culture) added with different concentrations of GDF11 (0ng / ml, 100ng / ml, 200ng / ml, 300ng / ml) 10% Australian fetal bovine serum, 0.5 mM dexamethasone, 10 μM insulin, 200 μM indomethacin, 0.5 mM 3-isobutyl-1-methyl (IBMX)) were added to culture the cells. Cell differentiation status was observed daily, and Oil Red O staining was performed after 14 days of culture. It was found that the addition of GDF11 significantly reduced the number and size of lipid droplets in the cells compared with the control group (0ng / ml GDF11), and the results were as follows Figure 1A , Figure 1B , Figure 1C and Figure 1D shown. Therefore, it can be considered...

Embodiment 2

[0092] Example 2 Effect of GDF11 on fat metabolism

[0093] 1. GDF11 reduces triglyceride accumulation and promotes lipolysis in adipocytes

[0094] In order to explore the effect of GDF11 on fat metabolism, adipocytes differentiated for 14 days were used in this example. Replaced with normal medium (DMEM+10% Australian fetus) and added GDF11 for 3 days, oil red O staining found that GDF11 treatment can significantly reduce the content of lipid droplets in adipocytes, the results are as follows Figure 3A , Figure 3B , Figure 3C and Figure 3D As shown, by detecting the content of triglycerides in cells ( Figure 4A ), also obtained the same result that GDF11 treatment reduced triglyceride content in adipocytes. The catabolism of triglycerides will eventually produce free fatty acids (FFA) and glycerol. Since the lack of glycerol kinase activity in adipose tissue cannot decompose or reuse glycerol, glycerol release is often used as an indirect indicator of adipocyte lip...

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Abstract

The invention provides the application of a GDF11 (Growth Differentiation Factor 11) in preparing a product for treating obesity and the product for treating obesity, and relates to the technical field of biomedicines. The GDF11 is capable of promoting proliferation of adipose-derived stem cells and inhibiting adipogenic differentiation of the adipose-derived stem cells. The GDF11 is also capableof inhibiting anabolism in fat cells and promoting catabolism of fat to reduce the content of triglyceride in the fat cells, and the result shows that the GDF11 has a certain inhibition function on obesity. The GDF11 is applied to the preparation of a product for treating obesity, and obesity can be effectively inhibited, so that an obesity treatment effect can be improved, and the GDF11 has the potential of being a new treatment medicine in deep obesity research. The invention provides a medicine for treating obesity, and the medicine comprises an active component of GDF11, so that the medicine is capable of effectively inhibiting obesity, has a remarkable prevention and / or treatment function on obesity, and meanwhile is safe and non-toxic and small in side effects.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to the application of GDF11 in the preparation of products for treating obesity and the products for treating obesity. Background technique [0002] Obesity is mainly a metabolic disease caused by the increase in the volume of fat cells in the body and the excessive accumulation of body fat due to the interaction of genetic, environmental (mainly diet) and psychosocial factors. According to relevant studies, obesity is a chronic inflammatory state, and adipose tissue will release various factors to induce diseases, such as hypertension, diabetes and cardiovascular diseases, dyslipidemia, atherosclerosis, stroke, etc. [0003] With the changes in living standards and social rhythms, both in developed and developing countries, obesity among children and adults is on the rise, and obesity has become a global problem. In China, from 1992 to 2015, the overweight rate increased from ...

Claims

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Application Information

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IPC IPC(8): A61K38/18A61P3/04
CPCA61K38/1841A61P3/04
Inventor 任哲王紫鋆刘俊伟陈静怡王一飞
Owner JINAN UNIVERSITY
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