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178 results about "Protease digestion" patented technology
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1. Proteases. Digestion of proteins is initiated by pepsin in the stomach, but the bulk of protein digestion is due to the pancreatic proteases. Several proteases are synthesized in the pancreas and secreted into the lumen of the small intestine.
Method and system for rapid biomolecular recognition of amino acids and protein sequencing
InactiveUS6846638B2Low costHigh sensitivityBioreactor/fermenter combinationsBiological substance pretreatmentsProtein Sequence DeterminationDigestion
Methods, compositions, kits, and apparatus are provided wherein the aminoacyl-tRNA synthetase system is used to analyze amino acids. The method allows very small devices for quantitative or semi-quantitative analysis of the amino acids in samples or in sequential or complete proteolytic digestions. The methods can be readily applied to the detection and / or quantitation of one or more primary amino acids by using cognate aminoacyl-tRNA synthetase and cognate tRNA. The basis of the method is that each of the 20 synthetases and / or a tRNA specific for a different amino acid is separated spatially or differentially labeled. The reactions catalyzed by all 20 synthetases may be monitored simultaneously, or nearly simultaneously, or in parallel. Each separately positioned synthetase or tRNA will signal its cognate amino acid. The synthetase reactions can be monitored using continuous spectroscopic assays. Alternatively, since elongation factor Tu:GTP (EF-Tu:GTP) specifically binds all AA-tRNAs, the aminoacylation reactions catalyzed by the synthetases can be monitored using ligand assays. Microarrays and microsensors for amino acid analysis are provided. Additionally, amino acid analysis devices are integrated with protease digestions to produce miniaturized enzymatic sequenators capable of generating either N- or C-terminal sequence and composition data for a protein or peptide. The possibility of parallel processing of many samples in an automated manner is discussed.
Owner:NANOBIODYNAMICS
Method and system for rapid biomolecular recognition of amino acids and protein sequencing
InactiveUS20050164264A1Quick analysisBioreactor/fermenter combinationsBiological substance pretreatmentsProtein Sequence DeterminationDigestion
Methods, compositions, kits, and apparatus are provided wherein the aminoacyl-tRNA synthetase system is used to analyze amino acids. The method allows very small devices for quantitative or semi-quantitative analysis of the amino acids in samples or in sequential or complete proteolytic digestions. The methods can be readily applied to the detection and / or quantitation of one or more primary amino acids by using cognate aminoacyl-tRNA synthetase and cognate tRNA. The basis of the method is that each of the 20 synthetases and / or a tRNA specific for a different amino acid is separated spatially or differentially labeled. The reactions catalyzed by all 20 synthetases may be monitored simultaneously, or nearly simultaneously, or in parallel. Each separately positioned synthetase or tRNA will signal its cognate amino acid. The synthetase reactions can be monitored using continuous spectroscopic assays. Alternatively, since elongation factor Tu:GTP (EF-Tu:GTP) specifically binds all AA−tRNAs, the aminoacylation reactions catalyzed by the synthetases can be monitored using ligand assays. Microarrays and microsensors for amino acid analysis are provided. Additionally, amino acid analysis devices are integrated with protease digestions to produce miniaturized enzymatic sequenators capable of generating either N- or C-terminal sequence and composition data for a protein or peptide. The possibility of parallel processing of many samples in an automated manner is discussed.
Owner:NANOBIODYNAMICS
Preparing method for heteroossein base materials
A process for preparing collagen matrix with heterogeneous bone, used as the bone repairing material and the extracellular matrix of bone tissue engineering, includes such steps as treating ox bone or pig bone, defatting, partial decalcifying, removing non-collagen, proteinas digesting, disinfecting and packing. It has better porosity and pore canal structure.
Owner:CHONGQING UNIV
Compositions for the detection of enzyme activity in biological samples and methods of use thereof
InactiveUS6936687B1Fluorescence enhancementFluorescent signal enhancementPeptide/protein ingredientsMicrobiological testing/measurementProteinase activityFluorophore
The present invention provides for novel reagents whose fluorescence increases in the presence of particular proteases. The reagents comprise a characteristically folded peptide backbone each end of which is conjugated to a fluorophore. When the folded peptide is cleaved, as by digestion with a protease, the fluorophores provide a high intensity fluorescent signal at a visible wavelength. Because of their high fluorescence signal in the visible wavelengths, these protease indicators are particularly well suited for detection of protease activity in biological samples, in particular in frozen tissue sections. Thus this invention also provides for methods of detecting protease activity in situ in frozen sections.
Owner:ONCOIMMUNIN
Direct determination of vitamin D in serum or plasma
A method for quantitating vitamin D metabolites directly in blood plasma or serum, without the need for prior purification of the vitamin D metabolites, comprising a digestion of the serum proteins with a serine protease such as proteinase K and sequence of steps for inhibiting the proteinase K activity in the competitive binding analysis. The advantages of this method are its high accuracy over the whole range of physiologically relevant values and that it can be easily adapted for a fully automated analysis of serum and plasma samples.
Owner:IMMUNDIAGNOSTIK AG
Method and apparatus for selective filtering of ions
ActiveUS7514676B1Reduce the impactReduce chargeStability-of-path spectrometersTime-of-flight spectrometersMass analyzerSpectrometer
An adjustable, low mass-to-charge (m / z) filter is disclosed employing electrospray ionization to block ions associated with unwanted low m / z species from entering the mass spectrometer and contributing their space charge to down-stream ion accumulation steps. The low-mass filter is made by using an adjustable potential energy barrier from the conductance limiting terminal electrode of an electrodynamic ion funnel, which prohibits species with higher ion mobilities from being transmitted. The filter provides a linear voltage adjustment of low-mass filtering from m / z values from about 50 to about 500. Mass filtering above m / z 500 can also be performed; however, higher m / z species are attenuated. The mass filter was evaluated with a liquid chromatography-mass spectrometry analysis of an albumin tryptic digest and resulted in the ability to block low-mass, “background” ions which account for 40-70% of the total ion current from the ESI source during peak elution.
Owner:BATTELLE MEMORIAL INST
Process for producing a soybean protein usable in acidic foods
InactiveUS7465470B2Loss in yieldGood storage stabilityProtein composition from vegetable seedsFood ingredientsSolubilityHydrolysate
It is intended to provide a soybean protein material which is excellent in solubility, stability, emulsifying properties and gel-forming properties under acidic conditions and thus advantageously usable in acidic foods, its production process, and acidic foods using the soybean protein material. A solution containing soybean protein is subjected to a treatment for eliminating or inactivating polyanionic substances contained therein and / or adding a polycationic substance and then heated at above 100° C. under acidic conditions. Thus, a soybean protein having a high solubility under acidic conditions and thus being appropriately usable in acidic foods can be obtained. By using this protein, protein foods in an acidic region can be provided. Further, by combining the above-described treatment with a protease-digestion treatment, a soybean protein hydrolysate having a high solubility in an acidic region can be efficiently obtained.
Owner:FUJI OIL CO LTD
Method for soluble expression of recombinant protein of human brain natriuretic peptide and application
ActiveCN103923937AHigh activitySimple and easy to repeatHormone peptidesPeptide/protein ingredientsProtein targetChaperonin
The invention provides a method for soluble expression of recombinant protein of a human brain natriuretic peptide (Human Brain Natriuretic Peptide, hBNP). The method comprises the following steps: 1) obtaining a recombinant gene, namely obtaining the recombinant gene His-DsbAmut-BNP of expressing the recombinant protein of the human brain natriuretic peptide, wherein the sequence of the recombinant gene comprises a purified tag gene sequence, a molecular chaperone protein gene sequence, a protease recognition site sequence and a human brain natriuretic peptide sequence, which are sequentially arranged along the direction from 5' to 3'; 2) obtaining recombinant plasmids, namely inserting the recombinant gene obtained in the previous step into a carrier vector, so as to obtain the recombinant plasmids containing the recombinant gene; 3) obtaining genetically engineered bacterium, transferring the recombinant plasmids obtained in the previous step into host cells to obtain the genetically engineered bacterium; 4) expressing exogenous genes, fermenting the genetically engineered bacterium, and expressing fusion protein containing the human brain natriuretic peptide; 5) purifying target protein, splitting thallus, collecting fusion protein, removing molecular chaperone and carrying out chromatographic purification by protease digestion, so as to obtain the recombinant protein of the human brain natriuretic peptide.
Owner:SHIJIAZHUANG WOTAI BIOTECH
Proteinase-engineered cancer vaccine induces immune responses to prevent cancer and to systemically kill cancer cells
InactiveUS20090162405A1Induce immune responseVaccinesCancer antigen ingredientsCancer preventionCancer cell
A harmless cancer vaccine is made from cancer cells with extracellular proteins including self-recognition molecular patterns being digested by a proteinase. The cancer vaccine is used to vaccinate an individual to induce immune responses against cancer cells systemically. Cancer cells become harmless when they are digested by Tumorase™. Some proteinases including trypsin cannot kill cancer cells completely and treated cancer cells need to be further processed in order to be harmless and effective. Cancer cells may be from tissue-cultured human or animal cancer cell lines or cancer patients directly. Cancer vaccine vaccinated individuals produce cancer vaccine specific immune responses against cancer cells. Immune response components may be isolated and used to fight against cancer for a cancer patient with a suppressed immune system. Cancer vaccine specific immune components may include cancer vaccine specific polyclonal antibodies, B-cells, T-cells, natural killer cells, monocytes, macrophages and other lymphocytes.
Owner:QIAN YONG
Activated protease indicator
InactiveUS20100297620A1Recovery functionCompound screeningApoptosis detectionProteinase activityBiological activation
It is an object of the invention to provide novel approaches capable of detecting activated protease and also detecting protease activation on real time at a high sensitivity in a noninvasive manner. By the method for detecting activated protease of the invention, an indicator in a circular form comprising the C-half fragment of luciferase (Luc-C) and the N-half fragment of luciferase (Luc-N) linked together through a substrate peptide for a protease is introduced in an in vitro assay system or in cells. Upon digestion of the substrate peptide by the protease, Luc-N and Luc-C together reconstructs active luciferase, so that the activated protease can be detected by assaying the luminescence signal from the luciferase.
Owner:THE UNIV OF TOKYO +1
Albumen microsphere conjugate for detecting acrosin activity and preparation method and application of albumen microsphere conjugate
InactiveCN106146642AAccurate evaluationRealize synchronous detectionSerum albuminFluorescence/phosphorescenceMicrosphereProtein labeling
The invention discloses an albumen microsphere conjugate for detecting acrosin activity and a preparation method and application of the albumen microsphere conjugate. The albumen microsphere conjugate is characterized in that monodisperse micron microspheres obtained by combining fluorescein and the acrosome reaction substrate, namely zona pellucida protein are prepared, the acrosin activity is detected by aid of change in fluorescence intensity, and the specific steps include preparation of monodisperse microspheres modified through surface carboxylation or amination, surface modificaction, fluorescence labeling of protein polypeptide, conjugation of fluorescent protein polypeptide and functional microspheres and detection of the acrosin activity. The albumen microsphere conjugate has the advantages that protease digestion on the microspheres is realized through the polymer microsphere technology and the protein labeling and conjugating technology, and enzymatic activity is evaluated; the albumen microsphere conjugate is mainly applied to flow detection of the acrosin activity, synchronous detection of an acrosome reaction and the acrosin activity can be realized, and acrosome functions can be evaluated more truthfully and accurately.
Owner:ZHEJIANG CELLPRO BIOTECH
Method for assaying glycated albumin
ActiveUS7871789B2Efficient digestionAssay the glycated albumin more efficientlyHydrolasesMicrobiological testing/measurementCarbon numberAlbuminous degeneration
An albumin denaturing agent for digesting an albumin by a protease efficiently is provided. The albumin denaturing agent contains quaternary ammonium having a hydrocarbon group with a carbon number of 12 or more, or a salt of the quaternary ammonium. The albumin in a sample is digested by the protease in the presence of the albumin denaturing agent, a glycated part of the thus obtained albumin digestion product and a FAOD effect a reaction, and a redox reaction between the glycated part and the FAOD is measured, thereby determining a ratio (GA (%)) of the glycated albumin of the glycated albumin with respect to the albumin.
Owner:ARKRAY INC
Serum-free medium of human amniotic epithelial stem cell and culture method thereof
ActiveCN106834218AOvercoming difficulties in obtaining materialsOvercome limitationsCell dissociation methodsCulture processCuticleSingle cell suspension
The invention relates to a serum-free medium of a human amniotic epithelial stem cell and a culture method thereof. The serum-free medium is formed by adding a human epidermal growth factor, human transferring, human insulin, sodium selenite, alanyl-L-glutamine dipeptide, alanine, asparaginate, aspartic acid, glutamic acid, glycine, proline and serine to a DMEM / F12 basic medium. The culture method comprises the steps of digesting 2.5g / L of trypsin for a human amniotic membrane, obtaining the human amniotic epithelial stem cell and filtering to prepare a single cell suspension; putting the human amniotic epithelial stem cell into a CO2 incubator of which the saturated humidity and the volume fraction are 5% at 37 DEG C through the serum-free medium under a serum-free condition and achieving growth and amplification of the amniotic epithelial stem cell through liquid exchange and subculture under the serum-free culture condition, the serum-free medium has the characteristics of stem cells, and is free of other animal sources, wide in source and free of ethical restriction.
Owner:沈阳艾米奥生物工程技术研发中心有限公司
Tunny head immunological activity substance and method of preparing the same
InactiveCN101249103AScientific and reasonable processMild responseSolution deliveryPill deliveryNeutral proteaseUltrafiltration
An immunoactive substance extracted from tuna fish head and a preparation method thereof are provided. The method comprises the following steps: pretreatment, washing, crashing, protease digestion, hydrolysis with neutral protease and papain, oil-water separation, hydrolysate centrifugation, oil-water separation of filtrate, separation of protein hydrolysate and fish oil, fractional separation, and separation of protein hydrolysate by using ultrafiltration membrane, to obtain immunoactive substance of tuna fish head. The invention utilizes two proteases to hydrolyze proteins of tuna fish head and centrifugally separates peptide-rich protein hydrolysate with immunological activity. The protein hydrolysate can be further processed to oral liquid, capsules and tablets. Furthermore, the invention produces fish oil rich in polyunsaturated fatty acids, thereby realizing the comprehensive and high-efficiency utilization of tuna fish head. The immunoactive substance of tuna fish head can significantly improve humoral immunity and nonspecific immunity in mice and enhance the cellular immune function of mice.
Owner:GUANGDONG OCEAN UNIVERSITY
Compositions for the detection of enzyme activity in biological samples and methods of use thereof
InactiveUS20080199898A1Fluorescent signal enhancementFluorescence enhancementMicrobiological testing/measurementBiological material analysisProteinase activityFluorophore
The present invention provides for novel reagents whose fluorescence increases in the presence of particular proteases. The reagents comprise a characteristically folded peptide backbone conjugated to two fluorophores such that the fluorophores are located opposite sides of a cleavage site. When the folded peptide is cleaved, as by digestion with a protease, the fluorophores provide a high intensity fluorescent signal at a visible wavelength. Because of their high specificity and their high fluorescence signal in the visible wavelengths, these protease indicators are particularly well suited for detection of protease activity in biological samples, in particular in frozen tissue sections. Thus this invention also provides for methods of detecting protease activity in situ in frozen sections.
Owner:ONCOIMMUNIN
Bifid yeast fermentation composition and preparation method thereof
InactiveCN112675081AReduce lossesGuaranteed concentrationCosmetic preparationsBacteriaBiotechnologyCulture mediums
The invention relates to a bifid yeast fermentation composition and a preparation method thereof. The fermentation composition is formed by mixing bifid yeast lysis substance mother liquor and filtrate according to a certain ratio. The preparation method comprises the following steps of: S1: carrying out anaerobic culture and activation to prepare a first-level fermentation seed solution; S2: inoculating the first-level fermentation seed solution into a micro-aerobic fermentation solid culture medium to obtain a second-level fermentation seed solution; S3: transferring the second-level fermentation seed solution into the fermentation culture medium to carry out micro-aerobic fermentation; S4: through fermentation broth centrifugation, separating bifid yeast filtrate from bifidobacterium bodies; S5: after the bifidobacterium bodies are resuspended and broken, obtaining the bifid yeast lysis substance mother liquor; and S6: mixing the bifid yeast filtrate with the lysis substance mother liquor to obtain a bifid yeast fermentation product. Since bifidobacterium longum is used for carrying out multilevel anaerobic / micro-aerobic fermentation, the problem of a microorganism pollution influence is restricted, and safety is further improved; indexes, including an oxygen content, are controlled in a step-by-step fermentation process, and each physicochemical index of a final product is stable; and through a compound protease digestion technology, the content of polypeptides under same-scale bifidobacterium fermentation is effectively improved.
Owner:天津强微特生物科技有限公司
Nucleic acid rapid-purifying method and kit
The invention discloses a nucleic acid rapid-purifying method and kit. Specifically, the present invention discloses a reagent combination used for rapid purification of nucleic acids from a nucleic acid-containing sample, the kit prepared on the basis of the reagent combination, and the nucleic acid rapid-purifying method on the basis of the reagent combination or the kit. The method of the invention has the advantages of high nucleic acid yield, high nucleic acid purity, rapidness, room temperature operation, no need of protease digestion process and the like, and is suitable for analysis or extraction and purification of the nucleic acids of various downstream experiments.
Owner:EMERGING THERAPEUTICS SHANGHAI CO LTD
Novel preparation technology of targeting antitumor fusion protein LPO (lipid peroxidation)
InactiveCN103805621AEnhanced inhibitory effectHybrid peptidesAntineoplastic agentsHistidineLipid peroxidation
The invention discloses a novel preparation technology of targeting antitumor fusion protein (lipid peroxidation). The novel preparation method comprises the steps of building a fusion expression protein which takes the glutathione S-transferase A (TrxA)-His-tag (His6-Tag)-SUMO protease recognition substrate as a protein soluble expression auxiliary fragment and takes LHRH (luteinizing hormone releasing hormone)-PEA (phenylethylamine) trans-cell penetrating peptides-ONC (LPO for short) as a target segment, connecting the two proteins with each other by a correct read frame and carrier positive sequence and converting to enter into expression bacteria, finally building fusion protein which is connected with six expression substances in series, crudely extracting, carrying out metal chelating medium purification in the presence of imidazole, and carrying out SUMO protease digestion. Compared with the LPO prepared by a conventional method, the LPO prepared by the novel preparation technology disclosed by the invention can obviously improve the inhibiting effect on the tumor cell lines such as colon cancer HT-29 cells, ovarian cancer OVCAR3 cells, cervical adenocarcinoma HeLa cells and liver cancer HepG-2 cells.
Owner:MILITARY VETERINARY RES INST PLA MILITARY MEDICAL ACAD OF SCI
Compositions and methods for treating or preventing overweight or obesity with zinc-charged protein fragments
InactiveUS20070004617A1Reduce weight gainModulating insulin receptor activityOrganic active ingredientsMetabolism disorderOverweightMilk protein
Compositions and methods for treating or preventing, for example, overweight or obesity are provided. Compositions provided comprise zinc-charged, protease digested serum or milk proteins. Compositions are administered in a therapeutically effective amount to treat or prevent, for example, overweight or obesity.
Owner:AMBRYX BIOTECHNOLOGY INC
Collagen peptide composition having good ability to enter the blood and food or beverage containing the same
InactiveUS20120040055A1Small amountProtein composition from fishMilk preparationHydroxyprolineDipeptide
An object of the present invention is to elucidate a collagen peptide effective for causing dipeptides or tripeptides serving as the active component to enter the blood, and thus to reduce the required intake thereof. According to the present invention, a collagen peptide composition obtained by digesting collagen or gelatin with protease is provided, wherein:(a) the ratio of hydroxyproline to total of amino acid residues at the second position from the N terminus of the peptides in the composition is 2 mol % or more and 20 mol % or less, and the ratio of glycine to total of amino acid residues at the third position from the N terminus of the peptides in the composition is 20 mol % or more and 50 mol % or less; and(b) the average molecular weight is 500 or more and 2000 or less.
Owner:MEIJI CO LTD
Method and apparatus for selective filtering of ions
ActiveUS20090108194A1Reduce chargeLower ratioTime-of-flight spectrometersMaterial analysis by electric/magnetic meansMass spectrometricSpectrometer
An adjustable, low mass-to-charge (m / z) filter is disclosed employing electrospray ionization to block ions associated with unwanted low m / z species from entering the mass spectrometer and contributing their space charge to down-stream ion accumulation steps. The low-mass filter is made by using an adjustable potential energy barrier from the conductance limiting terminal electrode of an electrodynamic ion funnel, which prohibits species with higher ion mobilities from being transmitted. The filter provides a linear voltage adjustment of low-mass filtering from m / z values from about 50 to about 500. Mass filtering above m / z 500 can also be performed; however, higher m / z species are attenuated. The mass filter was evaluated with a liquid chromatography-mass spectrometry analysis of an albumin tryptic digest and resulted in the ability to block low-mass, “background” ions which account for 40-70% of the total ion current from the ESI source during peak elution.
Owner:BATTELLE MEMORIAL INST
Method for culturing and amplifying odontogenic epithelial cells
ActiveCN102002477ASolve difficult problemsEasy to masterArtificial cell constructsVertebrate cellsOdontogenic EpitheliumDispase
The invention discloses a method for culturing and amplifying odontogenic epithelial cells, and belongs to the technical field of cell culture. The method for culturing and amplifying the odontogenic epithelial cells of the invention comprises the steps of obtaining and culturing primary epithelial cells and amplifying the epithelial cells, wherein the primary culture comprises the steps of digesting odontogenic chyle-shaped tissues by using mixed enzyme digestive juice consisting of dispersing enzyme, I-type collagenase and DNA enzyme, collecting cells and culturing; and the mixed enzyme comprises the dispersing enzyme, the I-type collagenase and the DNA enzyme; and sub-culturing comprises the steps of digesting the cells by combining a cell scraper scrapping method and a 0.025 to 0.25 percent trypsin digestive juice, suspending the cells again with a culture medium and culturing. By the method, the cells are sub-cultured for 2 to 3 times to form a great number of purified odontogenic epithelial cells which are identified to be epithelial cells by an epithelial cell expression marker CK14. The method solves the problem of difficulty in culturing the odontogenic epithelial cells, and a great number of stable and purified odontogenic epithelial cells are obtained and the source of the epithelial cells is provided for the teeth development and regeneration research.
Owner:成都世联康健生物科技有限公司
In-gel tagging and in-gel digestion for phosphoproteins analysis and phosphorylation site identification
The present invention relates to a method for phosphorylation site-specific labeling of phosphoproteome with a site-specific tagging reagent and analyzing of the resulting labeled one, more especially, a method for in-situ tagging of phosphorylation sites of phosphoproteins retained in polymeric gel with a nucleophilic tagging reagent. It also relates a method for generating new proteolytic cleavable sites at formerly phosphorylation sites by a proper choice of a nucleophilic tagging reagent. It also relates to a method for phosphopeptides analysis and phosphorylation site identification by in-gel digestion of the previously in-gel tagged proteins and subsequent mass analysis of the resulting peptides. The invention provides in-gel chemical tagging method for phosphoaminoacid residue of phosphoproteins retained in polymeric gel matrix. Phosphoprotein can be immobilized into gel matrix by a variety of methods such as gel electrophoresis. The immobilized phosphoproteins are retained in gel matrix during tagging reaction to phosphorylated aminoacid residue of phosphoproteins, and the resulting tagged proteins are also retained in gel matrix till following purification steps like washing of the tagging reagents are accomplished. The tagged proteins is digested by protease, and the resulting digested peptides is released from gel into solution and applied for peptide mass analysis.
Owner:KOREA BASIC SCI INST
Collagen peptide composition and food or beverage containing the same
InactiveUS20100068342A1Improve abilitiesSmall amountPeptide/protein ingredientsAntipyreticGlycineProteinase activity
An object of the present invention is to elucidate a collagen peptide composed of oligopeptides having ability to enter the blood higher than that of conventional collagen peptides and thus to provide a food or beverage mixed with the collagen peptide. The present invention provides a collagen peptide composition obtainable by digesting a collagen or gelatin with protease, which comprises 70% to 100% by weight of peptides with a molecular weight 500 or more to 3000 or less, less than 10% by weight of peptides with a molecular weight of less than 500, and less than 20% by weight of peptides with a molecular weight of more than 3000, based on the total weight of the composition, wherein the ratio of N-terminal glycine residues to total of the N-terminal amino acid residues of the peptides in the composition is 33 mol % or more to 65 mol % or less.
Owner:MEIJI CO LTD
Follicular stem cell originated from human hair and its amplifying prepn process
InactiveCN101045916AIncrease proliferative activityImprove anti-aging effectArtificially induced pluripotent cellsNon-embryonic pluripotent stem cellsCuticleTwo step
The present invention relates to follicular stem cell originated from human hair and its amplifying preparation process, and belongs to the field of medical preparation and human cell culture technology. The present invention adopts improved two-step protease digesting method to amplify follicular stem cell originated from human hair and obtains follicular stem cell with active expression of beta-integrin and keratin 19 in an optimized follicular stem cell preparing environment. The optimized micro environment endows follicular stem cell with fast amplification, antisenile characteristic, the epidermic cell-like, fibroblst-like and hair bulb tissue-like structure or functional expression. The present invention makes it possible to provide sufficient seed cell sources for the fast repair and functional reconstruction of skin defect.
Owner:TIANJIN HOSPITAL
Method and reagent for improving rate of extraction of DNAs of castoff cells in case trace sample
ActiveCN102229927AFacilitates efficient extractionFully lysedDNA preparationMagnetic beadPolyethylene glycol
Owner:深圳柏悦基因科技有限公司
Culture method of bone marrow derived endothelial progenitor cells
ActiveCN106754650ASolve problems with low yield and high priceDoes not affect culture efficiencyCulture processArtificial cell constructsSerum free mediaBasic fibroblast growth factor
The invention discloses a culture method of bone marrow derived endothelial progenitor cells and belongs to the technical field of cell culture. The method comprises the steps of treating extracted bone marrow with a red blood cell lysis buffer, then, carrying out separating so as to obtain single karyocytes of the bone marrow, carrying out washing, then, inoculating a fibronectin precoated culture dish with the obtained single karyocytes, carrying out culture by using a complete culture medium, digesting the cells with trypsin after a period of time of culture, inoculating culture bottles with digested cells, and carrying out differential adherent culture and differential digestion culture. The used complete culture medium is prepared through adding fetal calf serum, a vascular endothelial growth factor, glucocorticoid, vitamin C, an epidermal growth factor, a basic fibroblast growth factor, a colony stimulating factor and a platelet-derived growth factor into a serum-free medium EGM2. The method has the characteristics that the operating steps are relatively simple, the spent time is short, the quantity of separation in one time is large, the number of transfer generations of the obtained cells is large, and the like.
Owner:哈尔滨中科赛恩斯生物技术有限公司
Bactericidal and anti-itching medicated soap and preparation method thereof
InactiveCN110029026AHigh hardnessImprove wear resistanceSurface-active non-soap compounds and soap mixture detergentsSkin careGiant salamanderSea salt
The invention discloses a bactericidal and anti-itching medicated soap and a preparation method thereof. The medicated soap achieves bactericidal and bacteriostatic effects by fixing ozone to ozone salt and utilizing the efficient bactericidal action of the ozone; meanwhile, itching caused by skin allergy is reduced through a variety of Chinese herbal medicines and essential oil with the anti-itching effect; in addition, through mutual cooperation with a giant salamander mucus protein extract, horse oil, badger fat and ambergris, skin is nourished and itching and cracking caused by dryness areprevented. Through adoption of a formula of a neutral soap and supplemented by a foaming agent, during using, the medicated soap is smooth, delicate, non-irritant to the skin and good in skin friendliness; in addition, through addition of large-grained sea salt, the hardness and the plasticity of the medicated soap can be better and industrial production is more facilitated. Through protease digestion and use of natural plant raw materials during preparation, the safer factor of the medicated soap is higher and allergic risks are eliminated.
Owner:张慧君
Compositions for the detection of enzyme activity in biological samples and methods of use thereof
InactiveUS7312302B2Fluorescent signal enhancementFluorescence enhancementPeptide/protein ingredientsMicrobiological testing/measurementProteinase activityFluorophore
The present invention provides for novel reagents whose fluorescence increases in the presence of particular proteases. The reagents comprise a characteristically folded peptide backbone conjugated to two fluorophores such that the fluorophores are located opposite sides of a cleavage site. When the folded peptide is cleaved, as by digestion with a protease, the fluorophores provide a high intensity fluorescent signal at a visible wavelength. Because of their high specificity and their high fluorescence signal in the visible wavelengths, these protease indicators are particularly well suited for detection of protease activity in biological samples, in particular in frozen tissue sections. Thus this invention also provides for methods of detecting protease activity in situ in frozen sections.
Owner:ONCOIMMUNIN
Compositions and methods for treating or preventing overweight or obesity with zinc-charged protein fragments
InactiveUS7528108B2Modulating insulin receptor activityReduce weightOrganic active ingredientsMetabolism disorderSerum igeOverweight
Compositions and methods for treating or preventing, for example, overweight or obesity are provided. Compositions provided comprise zinc-charged, protease digested serum or milk proteins. Compositions are administered in a therapeutically effective amount to treat or prevent, for example, overweight or obesity.
Owner:AMBRYX BIOTECHNOLOGY INC
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