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Ultrasensitive fluorescence response method for detecting melamine in milk

A technology of melamine and milk, applied in the field of analytical chemistry, can solve the problems of derivatization, inability to apply rapid detection, lengthy analysis process, etc.

Inactive Publication Date: 2013-04-24
JILIN UNIV
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Problems solved by technology

[0006] Melamine itself has low toxicity, but melamine generates cyanuric acid under acidic or alkaline conditions. Melamine and its coexistent cyanuric acid form insoluble crystals in the kidney through hydrogen bonds, thus forming kidney stones. Long-term intake of high concentrations of cyanuric acid Melamine can cause kidney failure and even death, especially in children and adolescents
These methods have low detection limits and high sensitivity, but the analysis process is lengthy, requires derivatization, expensive instruments, and requires professional operators, so they cannot be applied to on-site rapid detection

Method used

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  • Ultrasensitive fluorescence response method for detecting melamine in milk
  • Ultrasensitive fluorescence response method for detecting melamine in milk
  • Ultrasensitive fluorescence response method for detecting melamine in milk

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Experimental program
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Embodiment Construction

[0041] Preparation of AuNPs

[0042] Materials / Reagents: Chloroauric acid and trisodium citrate purchased from Beijing Chemical Plant.

[0043] Method: The glassware used in the experiment was soaked in aqua regia for 24 hours, washed with twice distilled water, and dried for later use. The distilled water used in the preparation of the reagents had to be filtered through a 0.45 μm filter membrane; during preparation, 1 mmol / L of chlorine was added to the three-necked flask Auric acid 50mL, heat to boil while stirring, quickly add 38.8mmol / L trisodium citrate 5mL, stir while heating, the solution turns from light yellow to wine red, the reaction lasts for 10min, stop heating, continue stirring After 10 minutes, the solution was cooled to room temperature, filtered through a 0.45 μm microporous membrane, and stored at 4°C. The particle size of the prepared AuNPs was 13 nm; the prepared AuNPs were diluted with pure water at a volume ratio of 1:4 for later use. After the concent...

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Abstract

The invention relates to a method for detecting melamine in milk which is built based on the inner filter effect (IFE) between cysteine (L-Cys)-modified CdS quantum dots and citric acid ligand coating gold nanoparticles (AuNPs), and belongs to the field of analytical chemistry. The method comprises the detecting steps of preparing the AuNPs, synthesizing and purifying the L-Cys-modified CdS quantum dots, pre-treating a milk sample and detecting the melamine in the milk. According to the principle of recovering the fluorescence intensity of the CdS quantum dots by adopting the AuNPs to cause the fluorescence quenching of the CdS quantum dots and adopting the melamine to induce the aggregation of the AuNPs, the method can simply, quickly and sensitively detect the melamine in the milk so as to provide convenience to future dairy industry.

Description

technical field [0001] Utilizing the special chemical structure of melamine, milk based on the fluorescent inner filter effect (IFE) between cysteine ​​(L-Cys) modified CdS quantum dots and citric acid ligand-coated gold nanoparticles (AuNPs) The invention discloses a detection method for melamine, which belongs to the technical field of analytical chemistry. Background technique [0002] The chemical name of melamine is 2,4,6-triamino-1,3,5-triazine, and its structural formula is as follows: [0003] [0004] It is an important nitrogen heterocyclic organic chemical raw material, which is mainly applied in materials science. [0005] Because of its nitrogen content as high as 66.7%, it is often artificially added to food and animal feed to increase the N content. [0006] Melamine itself has low toxicity, but melamine generates cyanuric acid under acidic or alkaline conditions. Melamine and its coexistent cyanuric acid form insoluble crystals in the kidney through hy...

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N21/33
Inventor 孙春燕曹先一张民伟李宏坤郭佳佳沈斐罗叶丽
Owner JILIN UNIV
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