Method, solution and kit for simultaneously detecting multiple microbial genomes

Abstract

The invention discloses a method, solution and kit for simultaneously detecting multiple microbial genomes. The method comprises the following steps: high-throughput sequencing is carried out on a to-be-detected sample by adopting a microbial RNA detection process; before the sequencing, microbial cell lysis treatment is performed on a sample to be detected in advance, i.e., fungal cell wall lyticenzyme is adopted to perform cell wall lysis treatment on the sample to be detected, and meanwhile, an RNA enzyme inhibitor and dithiothreitol are added into a treatment solution; after cell wall cracking treatment is completed, physical grinding is further conducted; and then nucleic acid extraction and microbial RNA detection processes are carried out. According to the method disclosed by the invention, microbial genome nucleic acid including RNA and DNA in one sample can be comprehensively detected only through one process; the microbial genome detection cost and workload are reduced, andthe detection efficiency is improved; and the sample consumption is reduced, so that the microbial genome nucleic acid information of special samples or precious samples with a relatively small sampleamount can be obtained more completely.

Description

technical field [0001] The present application relates to the field of microbial genome detection, in particular to a method, solution and kit for simultaneously detecting multiple microbial genomes. Background technique [0002] Compared with conventional microbial detection methods, high-throughput sequencing technology can identify microbial species more quickly and accurately, and can simultaneously detect all microorganisms contained in a sample; therefore, high-throughput sequencing technology is gradually being widely used In the field of microbiological detection, especially for samples with unknown background conditions. [0003] Based on high-throughput sequencing technology, the genomes of multiple microorganisms in unknown samples can be detected simultaneously, that is, the simultaneous detection of multiple microbial genomes can be realized. However, in the face of unknown samples, the high-throughput sequencing technology for simultaneous detection of multipl...

AI Technical Summary

Problems solved by technology

However, after all, the main detection object of the microbial RNA detection process is RNA, and the DNA information is only partially residual, there will be many missed detections, and it is impossible to obtain complete DNA information; Microbial Genome Nucleic Acid Information

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