Method for extracting fungal oil

Abstract

The invention discloses a method for extracting fungal oil. The method comprises the steps of carrying out enzymolysis on a fungal cell wall through an enzyme-ultrasonic wave combined method (namely carrying out enzymolysis on the fungal cell wall by assisting chitinase and helicase by utilizing an ultrasonic wave); then adding SDS (Sodium Dodecyl Sulfate) to further split a fungal cell; adding isopropanol and n-pentane for one-time or multiple-time grease extraction. The method disclosed by the invention is simple in process, complete in fungal cell wall breaking and high in oil extraction rate as high as more than 95%. According to the method, a used main extraction solvent is relatively low in n-pentane boiling point and latent heat of vaporization and low in toxicity. Compared with the traditional fungal oil extraction method, the fungal oil extraction method disclosed by the invention has the advantages of low energy consumption and high environmental safety.

Description

technical field

[0001] The invention belongs to the field of bioengineering, and in particular relates to a method for extracting fungal oil. Background technique

[0002] With the decrease of fossil fuels, the energy problem has aroused widespread concern around the world, and the search for new renewable energy has become the only way. Fungal oil production has the advantages of short production cycle, high oil content, can use a variety of wastes to ferment oil, does not compete with humans for food, low production cost, safety and environmental protection, etc., and has a wide range of development and application potential.

[0003] Oleaginous fungi generally store oil in the cell. Therefore, in the process of oil extraction, the fungal cell must be broken first to release the oil to the outside of the cell, and then the oil can be extracted with a solvent. Therefore, the broken wall of the fungal cell The degree has a great influence on the extraction rate of fung...

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