Method for extracting fungal oil

An oil extraction and fungus technology, applied in the field of bioengineering, can solve the problems of time-consuming cell wall breaking, low wall breaking rate, affecting oil extraction efficiency, etc., and achieves large sample processing capacity, high environmental safety, energy saving and environmental protection boiling point. and the effect of latent heat of vaporization

Inactive Publication Date: 2014-05-14
INST OF DAFENG MARINE IND NANJING UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method breaks the cell wall by repeated freezing and thawing in the ultrasonic field. The cell wall breaking takes a long time, and the breaking rate is not high, which will affect the subsequent oil extraction efficiency.

Method used

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  • Method for extracting fungal oil

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Embodiment 1

[0031] References (Li Yonghong et al. Screening of broad-spectrum carbon source oleaginous yeast. China Biotechnology Journal, 2005, 25 (12): 39-44), the cultivation method of oleaginous yeast, cultivating Rhodosporidium Rhodosporidium toruloides (China Ordinary Microorganism Culture Collection and Management Center, No. AS2.1389), centrifuge at 4000rpm / min for 15min, and collect the wet bacteria. The oil content of the dried bacteria is 52.6% as measured by the classic method.

[0032] Refer to attached figure 1 A method for extracting fungal oil as shown, comprising the following steps: (1) cell culture: cultivating Rhodosporidium yeast (the same as the cultivation method of oleaginous yeast in the above reference); (2) cell collection: through a centrifuge Centrifuge the fungal culture solution, collect 20 g of wet fungal cells, and centrifuge at a centrifugation rate of 4000 rpm / min for 15 minutes; (3) enzymatic hydrolysis of the bacterial cell wall: dissolve the c...

Embodiment 2

[0035] References (He Rong et al., Agarita chrysogenum ( Umbelopsis isabellina ) Optimization of culture conditions for oil-producing fermentation of Hua 2-1. Journal of Applied and Environmental Biology, 2012, 18 (1): 80-85) The cultivation method of filamentous oleaginous fungus, cultivating Mortierella chrysogenum Mortierella isabellina (China Ordinary Microorganism Culture Collection and Management Center, No. AS3.3410), centrifuge at 2000rpm / min for 40min, and collect the wet bacteria. The oil content of the dried bacteria is 45.4% as measured by the classic method.

[0036] The difference with embodiment 1 is:

[0037] Step (1) Bacteria culture: Cultivate Mortierella chrysogenum (same as the method for culturing filamentous oleaginous fungi in the above-mentioned references);

[0038] Step (2) Cell collection: collect 10 g of wet fungal cells, centrifuge at 2000 rpm / min for 40 min;

[0039] Step (3) Cell wall enzymatic hydrolysis: sorbic acid 0.5mmol / ...

Embodiment 3

[0045] References (Li Yonghong et al., Screening of broad-spectrum carbon source oleaginous yeast. China Biotechnology Journal, 2005, 25 (12): 39-44), the cultivation method of oleaginous yeast, cultivating Rhodotorula viscosus Rhodotorula glutinis (China Ordinary Microorganism Culture Collection and Management Center, No. AS2.703), centrifuge at 6500rpm / min for 5min, and collect the wet bacteria. The oil content of the dried bacteria is 50.8% as measured by the classic method.

[0046] The difference with embodiment 1 is:

[0047] Step (1) Bacterial culture: Cultivate Rhodotorula viscosus (same as the method for cultivating oleaginous yeast in the above-mentioned references);

[0048] Step (2) Bacteria collection: centrifugation speed 6500rpm / min, centrifugation 5min;

[0049] Step (3) Enzymolysis of bacterial cell wall: sorbic acid 2mmol / L, CaCl2 20mmol / L, EDTA 0.15mmol / L, take 40mL of enzymolysis buffer solution, pH5.8, add 0.125g chitinase (0.8 million U / ...

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Abstract

The invention discloses a method for extracting fungal oil. The method comprises the steps of carrying out enzymolysis on a fungal cell wall through an enzyme-ultrasonic wave combined method (namely carrying out enzymolysis on the fungal cell wall by assisting chitinase and helicase by utilizing an ultrasonic wave); then adding SDS (Sodium Dodecyl Sulfate) to further split a fungal cell; adding isopropanol and n-pentane for one-time or multiple-time grease extraction. The method disclosed by the invention is simple in process, complete in fungal cell wall breaking and high in oil extraction rate as high as more than 95%. According to the method, a used main extraction solvent is relatively low in n-pentane boiling point and latent heat of vaporization and low in toxicity. Compared with the traditional fungal oil extraction method, the fungal oil extraction method disclosed by the invention has the advantages of low energy consumption and high environmental safety.

Description

technical field [0001] The invention belongs to the field of bioengineering, and in particular relates to a method for extracting fungal oil. Background technique [0002] With the decrease of fossil fuels, the energy problem has aroused widespread concern around the world, and the search for new renewable energy has become the only way. Fungal oil production has the advantages of short production cycle, high oil content, can use a variety of wastes to ferment oil, does not compete with humans for food, low production cost, safety and environmental protection, etc., and has a wide range of development and application potential. [0003] Oleaginous fungi generally store oil in the cell. Therefore, in the process of oil extraction, the fungal cell must be broken first to release the oil to the outside of the cell, and then the oil can be extracted with a solvent. Therefore, the broken wall of the fungal cell The degree has a great influence on the extraction rate of fung...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/10C11B1/02
Inventor 胡燕花陈集双张本厚
Owner INST OF DAFENG MARINE IND NANJING UNIV OF TECH
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