14 results about "Chitinase activity" patented technology

The present application provides a kind of shrimp shell biomass charcoal prepared by composite preparation and its preparation method and application, the present application is first prepared and obtained the composite preparation containing chitinase, bacillus licheniformis and bacillus subtilis, the chitinase is the chitinase derived from hartz wood enzyme is mutated, constructs mutant library, with trichoderma reesei as expression host, by screening, the excellent mutant Chi M1 obtained, its chitinase activity is significantly improved, is 2 times of original gene. By spraying the fermentation broth of the recombinant bacteria of the present application to obtain enzyme spray powder and the compounding of bacillus licheniformis powder and bacillus subtilis powder, the degradation efficiency of procambarus clarkia shrimp shell can be effectively improved. The composite preparation containing ChiM1 enzyme spray powder has good application prospect in the fields of aquaculture, feed additive and biomass resource utilization.

The invention discloses chitinase activity and application of a trehalose-6-phosphate synthase homologous protein. The trehalose-6-phosphate synthase is selected from any one of the following proteins: A1) a protein as shown in any one of SEQ ID NO.1-8; a2) a protein which is obtained by substitution and / or deletion and / or addition of one or more amino acid residues of the amino acid as shown in any one of SEQ ID NO.1-8 and has chitin synthase activity; a3) a protein derived from Trichoderma, yeast, Aspergillus nidulans, Aspergillus fumigatus, Candida albicans, Magnaporthe oryzae, Alternaria alternata and Escherichia coli, having 98% or more of identity with the protein of A1) and having chitin synthase activity; and A4) a fusion protein obtained by connecting a label to the N end or C end of the sequence A1) or A2) or A3). The Tpss disclosed by the invention has chitin synthase activity, can be used for synthesizing chitin from UDP-GlcNAc, and has a very good application prospect.

The present application relates to a compositions and methods comprising or expressing a hevamine A-related MoMo30 protein from Momordica balsamina. The MoMo30 protein is about 30 kDa in size, is stable after being autoclaved at 120° C. for 30 min, resists proteolytic cleavage by trypsin, exhibits mannose-sensitive binding to HIV gp120, exhibits hemagglutinin and chitinase activity, is capable of activating and stimulating T cell proliferation, is capable of preventing infection by HIV-1 or alleviating symptoms in an HIV-1 infected patients, and comprises an amino acid sequence of SEQ ID NO: 4. The MoMo30 protein and / or a nucleic acid encoding the same may be used in methods for preventing or treating microbial infections by HIV, SARS-CoV-2 and other enveloped viruses, as well as other microorganisms comprising cell surface proteins containing glycan residues, such as mannose.

The invention relates to the field of insect prevention and control, in particular to a high-stress-resistance beauveria bassiana strain CIPPBb075 with high pathogenicity to grassland caterpillars and application of the high-stress-resistance beauveria bassiana strain CIPPBb075. According to the invention, diseased insects parasitized by beauveria bassiana are collected from a field, and the strain is separated and purified, so that the CIPPBb075 strain has relatively high toxicity to grassland caterpillar; the germination rate is increased along with temperature rise, and the spore germination rate is obviously higher than that of other strains; the ultraviolet resistance at 25 DEG C is high; the extracellular protease activity is high; the activity of extracellular chitinase is high. Therefore, the CIPPBb075 strain has relatively strong stress resistance, and can be used as an effective microbial agent for preventing and treating the larvae of the grassland caterpillar compared with other strains.

The present invention provides a method for inhibiting Fusarium graminearum by symbiotic fermentation of Trichoderma and Metarhizium. The present invention obtains the best co-culture combination of dark green Trichoderma (Trichoderma atroviride) CJCCSJ-F-KY10039 and Metarhizium anisopliae (Metarhizium anisopliae) B81755 by screening different Trichoderma and Metarhizium, and the fermentation liquid obtained by symbiotic fermentation culture in liquid can inhibit Fusarium graminearum, the inoculation order of Trichoderma and Metarhizium is simultaneous inoculation, the two bacteria are inoculated at an inoculation ratio of 1:1, the chitinase activity in the fermentation liquid is 242.72IU / L, the chitosanase activity is 269.86U / L, the β-glucanase activity is 497.53U / L, and the inhibition rate of Fusarium graminearum is 63.3%.

The present application relates to the field of insect control, in particular to a strong stress-resistant Beauveria bassiana strain CIPPBb075 with high pathogenicity to lema frisoni and its application. The present application is collected from the field by the diseased insects parasitized by the Beauveria bassiana, and the strain is separated and purified, the CIPPBb075 strain has high virulence to lema frisoni; the germination rate increases with the increase of temperature, and the spore germination rate is significantly higher than that of other strains; the ultraviolet resistance is high at 25 DEG C; the extracellular protease activity is high; the extracellular chitinase activity is high. Therefore, the CIPPBb075 strain has strong stress resistance and other strains, and can be used as an effective microbial source drug for controlling lema frisoni larvae.

The present application discloses polypeptides and their uses, methods for preparing polypeptides, compositions or kits. The amino acid sequence of the polypeptide is shown as any one of SEQ ID NO: 1 to SEQ ID NO: 4; the method includes: culturing the recombinant cell described in the fourth aspect to obtain the polypeptide. The polypeptide of the present application has PETase activity and chitinase activity, can be used as a bifunctional hydrolase with PETase activity and chitinase activity, and has excellent thermal stability; the polypeptide of the present application can be highly expressed in a protein expression system. Thus, the method of the present application can be produced on a large scale and standardized; the use of the composition or kit containing the above polypeptide plays an important role in biological control, medical applications, waste treatment and environmental protection, etc.

According to the green tea extraction process provided by the invention, the extraction efficiency and quality of active ingredients of tea leaves are remarkably improved through combination of pulsed electric field pretreatment and a two-stage enzymolysis technology. The pulsed electric field enhances the cell permeability; cellulase and pectinase degrade cell walls in stages and release intracellular substances; as a natural activator, saccharicterpenin stabilizes chitinase activity and enhances antioxidant protection. And in the enzymolysis process, near infrared spectrum is combined to monitor the dissolution dynamic state of tea polyphenol in real time, so that the enzymolysis end point is accurately controlled, the obtained extract tea is high in active ingredient and excellent in oxidation resistance, and the batch stability is ensured.

The invention belongs to the technical field of probiotics, and particularly relates to bifidobacterium animalis XY01 with an inflammation relieving effect and application of the bifidobacterium animalis XY01. A novel Bifidobacterium animalis XY01 strain is separated from faeces of a healthy infant in Guangzhou city, Guangdong province, and research finds that the strain can inhibit aggregation of in-vivo macrophages at an inflammation part, promote subtraction of the macrophages from the inflammation part, reduce expression of in-vivo IL-6, IL-1beta and TNF-alpha, reduce hyaluronidase activity and inhibit inflammation of the infant. The chitinase activity is improved, and the potential inflammation relieving effect is achieved. Therefore, the bacterium has huge potential in the aspects of treatment and prevention of inflammation-related diseases, can be used for developing novel anti-inflammatory drugs, can also be used as a functional component to be added into health-care products, is beneficial to relieving inflammatory response and maintaining human health, and provides a new way and method for improving inflammation-related health problems.

The application discloses two strains of endophytic bacillus and application thereof in promoting plant growth and resisting blue-rot disease, and belongs to the field of plant growth promoting bacteria. The two strains of endophytic bacillus are isolated from tissue samples of healthy Pinus tabulaeformis and identified as bacillus, and the microbial preservation numbers thereof are CGMCC No. 37659 and CGMCC No. 37660 respectively. The two strains of bacillus both show strong inhibitory activity to a pathogenic fungus of blue-rot disease and can produce siderophores; one of the strains has the ability to produce IAA and does not have chitinase activity; the other strain does not produce IAA but produces chitinase; the two strains of bacillus have both commonness and complementarity in function, and the combination of the two strains can effectively promote the early germination of Pinus tabulaeformis seeds, significantly increase the biomass of Pinus tabulaeformis and significantly improve the resistance of the host plant to blue-rot fungus L. procerum , and has a significant prevention and treatment effect on the blue-rot disease of the plant caused by the blue-rot fungus.

In the present invention, chitinase enzymes are provided. Additionally, compositions comprising chitinase enzymes and methods of making and using chitinase enzymes are provided.

The present application relates to the technical field of microorganism, and particularly relates to a conical arthrobotus AC-ChiE01 strain and application thereof. The present application provides a conical arthrobotus AC-ChiE01 strain, the chitinase activity of the strain is significantly higher than that of a wild type strain, and the predation efficiency on the second instar larvae of horse roundworm is also significantly improved compared with the wild type strain. In addition, the conical arthrobotus AC-ChiE01 strain provided by the present application has good growth characteristics, and the sporulation capacity of the strain does not have obvious disadvantages compared with the wild type strain, and the conical arthrobotus AC-ChiE01 strain has a broad application prospect in the biological control of livestock digestive tract nematodiasis.

The invention relates to the technical field of microorganisms, and discloses an anti-ultraviolet high-toxicity beauveria bassiana mutant strain F5-Bb20240406-6 and an application of the mutant strain F5-Bb20240406-6. The beauveria bassiana mutant strain F5-Bb20240406-6 is obtained by carrying out ultraviolet mutagenesis screening on the beauveria bassiana Bb20240406, the lethal time of the mutant strain on tenebrio molitor, peanut aphid and other test insects is remarkably shortened, the biological characteristic is excellent, the sporulation quantity, the growth speed and the ultraviolet resistance are improved, and the activity of protease and chitinase is remarkably enhanced. The strain is applied to peanut pest control, has a good control effect on grubs and aphids, has remarkable leaf protection and yield increase effects, provides a high-quality strain resource for biological control of peanut pests, is beneficial to reducing the use of chemical pesticides, and promotes agricultural sustainable development.

The invention discloses a corn endophytic pseudomonas putida strain and application thereof. The corn endophytic pseudomonas putida strain is named as Pseudomonas putida SF07 and is preserved in the China Center for Type Culture Collection, the preservation starting date is September 26, 2024, and the preservation number is M 20242084; the preservation address is Wuhan University, Wuhan, China. The corn endophytic pseudomonas putida strain disclosed by the invention can be used for effectively inhibiting the growth of bipolaris maydis and setosphaeria turcica, has relatively good chitinase activity and relatively low cellulase and pectinase activity, is difficult to degrade plant cell walls, is relatively friendly to plants, and can be used as a biopesticide for preventing and treating corn diseases.