38 results about "Chitinase activity" patented technology

The present invention provides isolated nucleic acids encoding polypeptides with chitinase activity and methods for using the nucleic acids to enhance resistance of plants to fungal and nematode infections.

The present invention provides compositions with chitinase activity and methods for using such compositions to enhance resistance of plants to fungal and nematode infections.

This invention relates to pesticide and antiparasitic compositions for the control of pests, diseases and parasites attacking plants and animals. The compositions include, at least one chitinolytic agent or a chitinolytic activity-inducing agent, and sulfide or a sulfide-producing agent from microorganisms or chemical compounds, wherein the chitinolytic agent or the chitinolytic activity-inducing agent and sulfur or a sulfur-producing agent obtaining from microorganisms or chemical compounds are concurrently applied at a range significantly lower than any of the above-mentioned compounds, when they are individually to attain effective control.

The invention relates to the application of trichoderma strains in preparation of biopesticide for preventing and controlling cucumber fusarium wilt. The trichoderma strains are Trichoderma harzianum SH2303CGMCC NO.4963. The main components for preventing and controlling the cucumber fusarium wilt are conidia of the Trichoderma harzianum SH2303; and the biopesticide can be seed coating agents or granules. The trichoderma strains in the application has high growth speed and high sporulation quantity; the confrontation pathogens inhibition rate in vitro reaches 43.01 percent; the inhibition rate of a non-volatile inhibitor to the cucumber fusarium wilt is 41.33 percent; the inhibition rate of a volatile inhibitor to the cucumber fusarium wilt is 11.49 percent; the chitinase activity is 3.9573U; the beta-1,3-glucanase enzyme activity is 0.5092U; the extracellular protease enzyme activity is 3.0678 U; the prevention effect aiming at the living bodies of the cucumber fusarium wilt is 71.43 percent; the overall proportion of the antibiosis secondary metabolites, having antagonism, in the strains is 30.28 percent; and the trichoderma strains can effectively prevent and treat the disease of the cucumber fusarium wilt.

The invention discloses a disease-prevention and growth-promoting Trichoderma harzianum and an application thereof. The strain number of the Trichoderma harzianum is TM2-4, which is registered in theGeneral Microbiology Center of China Microbial Culture Collection Management Committee as CGMCC No. 15881. The Trichoderma harzianum M2-4 can inhibit Botrytis cinerea, Prunus persica, Colletotrichum gloeosporioides and Fusarium oxysporum f. Sp. Cucumerinum on fruits and vegetables, and has good control effect on tomato gray mold in vitro and can promote the germination of tomato seeds and the growth of tomato plants. The Trichoderma harzianum TM2-4 has indoleacetic acid (IAA)-producing activity, ferrophilic activity, glucanase and chitinase-producing activity. The Trichoderma harzianum TM2-4 has high breeding speed and can be cultured artificially, and the culture condition is simple, and the spore production is up to 3.2 * 109 cfu / g on the solid fermentation medium of wheat bran and corncob nutrient solution.

The invention discloses a detection method for disease resistance of cotton verticillium wilt. The detection method comprises the following steps: inoculating a verticillium wilt bacterium into a cotton seedling, detecting the activity of chitinase and / or activity of dextranase in the inoculated cotton seedling; and judging the disease resistance of cotton verticillium wilt according to the activity of chitinase and / or activity of dextranase in the inoculated cotton seedling. According to the detection method, the disease resistance of cotton verticillium wilt is judged by inoculating the verticillium wilt bacterium into the cotton seedling and detecting the activity of chitinase and / or the activity of dextranase in leaves of the inoculated cotton seedling. The method can be used for quickly and accurately acquiring the disease resistance of cotton verticillium wilt in the seedling growing stage without waiting for the cotton seedling to disease. A plant with good disease resistance or tolerance to diseases can be transplanted according to the detection result of the method. Through verification and screening in field morbidity, the anti-disease cotton plants and lines are obtained. The detection method is capable of conveniently obtaining single strains and materials with good disease resistance or tolerance to diseases from the seedling growing stage for direct high yield and quality breeding so as to accelerate the variety breeding process, so that the detection method is suitable for being popularized and applied.

The invention provides a biological compound potash fertilizer and a preparation method of the biological compound potash fertilizer. The biological compound potash fertilizer is prepared by the following steps of: fermenting pathogenic fungus CGMCC (China General Microbiological Culture Collection Center) No.5155 which resists 12.5% of potash and has chitinase activity to obtain a biological potash fertilizer; and mixing 8-15 parts of the biological potash fertilizer, 1-5 parts of dipotassium phosphate and 10-15 parts of humic acid potash fertilizer to prepare the biological compound potash fertilizer. The biological compound potash fertilizer provided by the invention is simple in preparation method, strong in product compositionality and strong in parataxis and has the functions of evenly providing nutrients, improving soil fertility, strengthening the stress resistance of crops, decreasing the crop diseases, improving the quality of agricultural products, decreasing the harmful substances in the agricultural products and the like.

The invention discloses fusion chitinase capable of efficiently degrading alpha-chitin and a related biological material and application thereof. The fusion chitinase is a protein obtained by fusing beta-N-acetyl hexosaminase and chitinase, wherein the chitinase activity of the fusion chitinase is higher than that of the beta-N-acetyl hexosaminase and that of the chitinase; the chitinase is D1) or D2), wherein the D1) is a protein with 53rd-322nd amino acid residues of which the amino acid sequence is shown in SEQ ID No.2; and the D2) is a fusion protein obtained by fusing a protein tag at the carboxyl terminal or / and the amino terminal of the protein shown in D1). The fusion chitinase has a degradation effect on various types of chitin, and has relatively high activity on colloid chitin, alpha-chitin and fungal cell wall chitin; and the fusion chitinase has the capability of completely hydrolyzing chitin and generating N-acetyl glucosamine. The fusion chitinase can be widely applied to biological medicine, biological agriculture, cosmetics, energy industry, etc.

The present invention provides an enzymatic composition comprising at least one protein isolated from a tissue or soup of a carnivorous plant, the at least one protein being characterized with an endo-chitinase activity.

The present invention is a method for preparing a biocontrol formulation by the following steps. A Streptomyces bacterium is selected simultaneously with superior sporulation ability, chitinase activity and antagonistic effectiveness against plant fungal pathogens by culturing with fungal pathogens, wherein the selected Streptomyces bacterium is cultured on a chitin-limited plate to assess the ability of sporulation and chitinase activity, and the Streptomyces bacterium with superior sporulation ability is the strain with producing powder-like and maturity form spore chains. Spores from the selected bacterium is collected. The obtained spores are taken as a seed inoculum. Then the seed inoculum is cultured to directly yield a broth medium containing at least 109 viable spores / ml which the spores contained in the broth medium are well suspended. The biocontrol formulation is obtained. The present invention further provided a biocontrol formulation containing a high concentration of Streptomyces spp. spores.

The invention discloses a culture medium for improving chitinase produced by streptomyces sampsonii KJ40 and a chitinase bacteria-containing preparation and application. The culture medium is composed of 100ml / L of colloid chitin with solubility of 2%(w / V), 0.4wt% of soybean cake powder, 1.0(g / L) of K2HPO4, and 0.6(g / L) of KH2PO4. Compared with an enzyme production basic culture medium, the activity of the chitinase produced according to new formulation and fermentation condition disclosed by the invention is raised by 80%. The chitinase bacteria-containing preparation with 50-100 times (volume ratio) dilution can be used for performing root-irrigation on breadth of root system of poplar nursery stock with 100ml for each poplar, and is respectively applied for once in spring and summer so as to prevent violet root rot of the poplar.

The invention relates to a method for prevention and treatment of diseases of longan fruits, wherein the method comprises the steps: (1) before picking, from the beginning of a longan young fruit stage, spraying a disease prevention and treatment liquid on longan; (2) when the maturity of the longan fruits is 80-90%, picking the longan fruits; and (3) soaking the longan fruits after picking in slightly acidic electrolyzed water, soaking for 10-15 min, then taking out, draining water, then carrying out fresh-keeping packaging on the longan fruits after draining, placing the packaged longan fruits under conditions of the temperature of 25-30 DEG C and the relative humidity of 85%-90%, and storing. The method for prevention and treatment of diseases of the longan fruits can improve the longan peel chitinase activity, beta-1, 3-glucanase activity, and the activity of phenylalanine ammonia lyase and other decrease-resistant related enzymes, and delays the decrease of the total phenolic content, so as to effectively control the occurrence of the diseases of postharvest longan fruits.

The present invention discloses endochitinase and a coding gene and application thereof in production of chitobiose. The protein is as follows: (a) or (b) or (c), wherein (a) is a protein comprising 40th-site to 696th-site amino acid residues from N-terminal of a sequence 2; (b) is a protein comprising an amino acid sequence shown as the sequence 2 in a sequence table; and (c) is a chitinase-activity protein which is derived from a sequence 1 and obtained by substitution and / or deletion and / or addition of one or more amino acid residues of the amino acid sequence of the (a) or (b). When the endochitinase is used for production of the chitobiose, conversion rate is high, energy consumption is less, no pollutant is produced, and the endochitinase has important economic value. The protein has a large potential for industrial application.

Excess sludge production in a bioreactor of a wastewater treatment plant in which excess sludge is being produced is reduced by adding an activated sludge material having a chitinase specific activity of at least 150 Units / g-MLSS and a pectinase specific activity of at least 120 Units / g-MLSS to the bioreactor. After the addition of the activated sludge material, the bioreactor is controlled by adding the activated sludge material when any one of the chitinase activity, the pectinase activity, and the protease specific of the sludge in the bioreactor drops below the lower limits of 50 Units / L, 40 Units / L, and 0.3 Units / L, respectively.

The invention relates to a fungal fermentation culture medium and process. More particularly, the invention relates to a culture medium and method for producing chitinase by Lecanicillium fermentation. The formula of the culture medium comprises the following raw materials: 5-15g / L of fructose or maltose, 5-15g / L of yeast extract powder or yeast extract extractum, 2-8*10<-4>mol / L of K<+> or Ca<2+>, 0.05-0.2g / L of Vc or VB6, and the balance of water; and the initial pH of the culture medium is 5.5-7.0. According to the method, a fermented seed solution is inoculated to the culture medium, the liquid containing quantity of a 250 mL triangular flask is 50 mL, the inoculum size is 15mL, and chitinase is cultured at 26 DEG C at the rotational speed of 160 r / min. The fermentation method is short in fermentation period, mild in condition, and easy to control; and the culture medium provided for producing chitinase by Lecanicillium fermentation has the advantages that the chitinase yield is high, the chitinase activity is high, and the like.

The present invention is a method for preparing a biocontrol formulation by the following steps. AStreptomyces bacterium is selected simultaneously with superior sporulation ability, chitinase activity and antagonistic effectiveness against plant fungal pathogens by culturing with fungal pathogens, wherein the selected Streptomyces bacterium is cultured on a chitin-limited plate to assess the ability of sporulation and chitinase activity, and the Streptomyces bacterium with superior sporulation ability is the strain with producing powder-like and maturity form spore chains. Spores from the selected bacterium is collected. The obtained spores are taken as a seed inoculum. Then the seed inoculum is cultured to directly yield a broth medium containing at least 109 viable spores / ml which the spores contained in the broth medium are well suspended. The biocontrol formulation is obtained. The present invention further provided a biocontrol formulation containing a high concentration of Streptomyces spp. spores.

This invention discloses the interference plasmid pHX RNAi applied in specificity silent gene in filamentous fungi. The plasmid includes an integrated hygromycin resistance gene expression cassette, a promotor from penicllium oxalicum and an intron structure from penicllium oxalicum; the nucleotide sequence of the interference plasmid is shown in SEQ iD No. 1. This invention also publicizes the application of the plasmid in the single-gene or polygenes expression silence that is difficult to knock out in the filamentous fungi penicllium oxalicum. This invention sets the amylase gene amy15A as the example to prove the high stability of the interference plasmid pHX RNAi and sets the amy15A and cel7A-2 double genes interference as the example to verify the advantages of pHX RNAi in applying for polygene silence. SDS-PAGE chitinase activity and RT-qPCR testing result indicate that RNA interference plays a remarkable and stable role in the post-transcription level, which predicts the promising application prospect of the interference plasmid in filamentous fungi.

The invention relates to a streptomycete for producing chitinase and an application thereof. An object of the invention is to solve the problem that conventional methods for controlling soybean gray mold and black spot diseases have hidden danger of pesticide residues. The streptomycete for producing the chitinase is streptomycete (Streptomyces sp.) G6, and is preserved in a general microbiology center of China Committee for Culture Collection of Microorganisms, and a preservation address is No.3, No.1 yard, Beichen west road, Chaoyang District, Beijing, a preservation date is February 02, 2018, and a preservation number is CGMCC No.15334. The application of the streptomycete for producing the chitinase refers to the application of prevention and control of soybean diseases. The bacterialstrain G6 not only has chitinase activity, but also has a good antagonistic effect on the soybean gray mold and black spot diseases, is free of the pesticide residues, and does not produce drug resistance. The streptomycete for producing the chitinase and the application thereof are applied to the technical field of environmental microorganisms.

Excess sludge production in a bioreactor of a wastewater treatment plant in which excess sludge is being produced is reduced by adding an activated sludge material having a chitinase specific activity of at least 150 Units / g-MLSS and a pectinase specific activity of at least 120 Units / g-MLSS to the bioreactor. After the addition of the activated sludge material, the bioreactor is controlled by adding the activated sludge material when any one of the chitinase activity, the pectinase activity, and the protease specific of the sludge in the bioreactor drops below the lower limits of 50 Units / L, 40 Units / L, and 0.3 Units / L, respectively.

This invention relates to pesticide and antiparasitic compositions for the control of pests, diseases and parasites attacking plants and animals. The compositions include, at least one chitinolytic agent or a chitinolytic activity-inducing agent, and sulfide or a sulfide-producing agent from microorganisms or chemical compounds, wherein the chitinolytic agent or the chitinolytic activity-inducing agent and sulfur or a sulfur-producing agent obtaining from microorganisms or chemical compounds are concurrently applied at a range significantly lower than any of the above-mentioned compounds, when they are individually to attain effective control.

The invention provides a chitinase-producing Bacillus subtilis strain SH21, a method for producing chitinase, microbial preparations and applications. The Bacillus subtilis strain SH21 has chitinase-producing activity. The chitinase activity of the Bacillus subtilis strain after fermentation can reach 4.02U / mL, and after the fermentation product is purified by a combination of salting out, gel filtration chromatography and ion exchange chromatography, the purification factor of chitinase is increased by 7.23 times, the specific activity is 154.5U / mg. The present invention also provides the application of the bacillus subtilis strain or microbial preparation in inhibiting the growth of Fusarium solani.

The invention belongs to the field of microorganisms and biotechnology, and in particular relates to a compound bacterial agent of microorganisms and its application in increasing production and quality of Angelica sinensis against diseases. The compound microbial agent is composed of Pseudomonas fluorescens, Pseudomonas alcaligenes and Pseudomonas psychrophilus; it has ACC deaminase activity, SOD activity, POD activity, NEX neutral xylan Enzyme activity, chitinase activity, and laccase activity; can promote the growth of the roots of the underground medicinal organs of Angelica sinensis, increase the diameter of the reed head, the length of the reed head, the length of the main root, and the average weight per plant, not only increasing the production but also improving the quality of commercial Angelica sinensis. Appearance properties; can increase the content of ferulic acid, Z-ligustilide, ligustilide A, endogenous jasmonic acid, salicylic acid, etc., which can significantly improve the quality of Angelica sinensis, and can be used to prepare special microorganisms for Angelica sinensis Fertilizer; it can prevent and control plant diseases caused by pathogenic fungi of the genus Fusarium, and can be used to prepare pesticides for preventing and controlling plant diseases.

A strain of streptomyces producing chitinase and its application, the invention relates to a strain of streptomyces producing chitinase and its application. The purpose of the present invention is to solve the problem that existing methods for controlling soybean gray mold and black spot have hidden dangers of pesticide residues. A strain of chitinase-producing Streptomyces of the present invention is Streptomyces (Streptomyces sp.) G6 , preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee, the preservation address is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, the preservation date is February 2, 2018, and the preservation number is CGMCC No.15334. The application of the chitinase-producing streptomyces of the present invention refers to the application in the prevention and control of soybean diseases. Strain G6 not only has chitinase activity, but also has good antagonistic effect on soybean gray mold and black spot, has no pesticide residue, and does not produce drug resistance. The invention is applied to the technical field of environmental microorganisms.

There is provided a method of treating hay for preventing and / or reducing heat damage in high moisture hay and as well to preserve the same, the method including adding to hay a hay preservative including a hay preserving and heat reducing effective amount of at least one enzyme having a chitinase activity, either alone or in combination with a hay preserving and heat reducing effective amount of a yeast of the genus Pichia or a bacteria of the genus Pediococcus. There is also provided a method of treating hay for preventing and / or reducing heat damage in high moisture hay and as well to preserve the same, the method including adding to hay a hay preservative including a hay preserving and heat reducing effective amount of yeast of the genus Pichia.

The invention discloses a method for fermenting and producing chitinase by Chaetomium globosa and its application. The strain is activated by using Chaetomium globosa HY1, and the activation medium includes glucose, peptone, potassium dihydrogen phosphate, dihydrogen phosphate Sodium; then shaker culture or fermenter culture, the fermentation medium includes soluble starch, glycerol, tryptone, yeast powder, corn steep liquor, NH 4 NO 3 , MnSO 4 , MgSO 4 、Na 2 HPO 4 、K 2 HPO 4 , Colloidal chitin, the chitinase produced by fermentation is used for the preparation of chitooligosaccharides. The cultivation method of the invention is simple, the nutritional requirements are not high, the chitinase produced by liquid fermentation has high activity, and has great industrial application value.

The invention provides a biological compound potash fertilizer and a preparation method of the biological compound potash fertilizer. The biological compound potash fertilizer is prepared by the following steps of: fermenting pathogenic fungus CGMCC (China General Microbiological Culture Collection Center) No.5155 which resists 12.5% of potash and has chitinase activity to obtain a biological potash fertilizer; and mixing 8-15 parts of the biological potash fertilizer, 1-5 parts of dipotassium phosphate and 10-15 parts of humic acid potash fertilizer to prepare the biological compound potash fertilizer. The biological compound potash fertilizer provided by the invention is simple in preparation method, strong in product compositionality and strong in parataxis and has the functions of evenly providing nutrients, improving soil fertility, strengthening the stress resistance of crops, decreasing the crop diseases, improving the quality of agricultural products, decreasing the harmful substances in the agricultural products and the like.

The invention relates to a long-acting composite enzyme disinfection composition for children and an application thereof. The disinfection composition consists of a composite enzyme dextrin inclusioncompound, a positively charged cationic polymerization group, a stabilizing agent, a humectant and the like, wherein the composite enzymes comprise one or two of lysozyme and lysostaphin, and one or two of chitinase and snailase; and in each gram of the composite enzyme dextrin inclusion compound, the concentration of the lysozyme is 100-200mg / g, the concentration of the lysostaphin is 0.1-1mg / g,the concentration of the chitinase is 100-200mg / g, and the concentration of the snailase is 10-100mg / g. The long-acting composite enzyme disinfection composition for children can for be released slowly for resisting bacteria, can prolong activity and stress resistance of the composite enzymes in a disinfection product in a use environment, and can realize a long-acting antibacterial effect. In addition, the composition has the advantages of wide sterilization spectrum, small skin irritation and no skin coloring problem, has high durability, and can be suitable for both of adults and children with delicate skins.

The invention discloses a African Trichoderma harzianum for disease prevention and growth promotion and application thereof. The strain number of Trichoderma harzianum is TM2-4, and its registration number in the General Microorganism Center of China Committee for Culture Collection of Microorganisms is CGMCC No.15881. Trichoderma harzianum M2‑4 has inhibitory effects on tomato cinerea, peach brown rot, pepper anthracnose and cucumber wilt on fruit and vegetable crops; Germination and plant growth are promoted. Trichoderma harzianum TM2‑4 has indoleacetic acid (IAA)-producing activity, siderophilic activity, glucanase-producing activity and chitinase-producing activity. Trichoderma harzianum TM2‑4 has a fast propagation speed, can be artificially cultured, and the culture conditions are simple. The highest spore production in the bran corncob nutrient solution solid-state fermentation medium can reach 3.2×10 9 cfu / g solid bacterial agent.