Stable and easy-to-store vaginal secretion microbial cell fluorescence detection dye liquor

Abstract

The invention discloses vaginal secretion microbial cell fluorescence detection dye liquor which is easy and convenient to operate, clear in dyeing effect, high in detection rate, not prone to precipitation, stable in system and easy to store. The vaginal secretion microbial cell fluorescence detection dye liquor comprises two reagents: a solution A and a solution B, fluorescein in the solution Acan be combined with beta-polysaccharide, such as chitin and cellulose, on fungal cell walls with high affinity, and fungal components existing in a sample are marked. The solution B is composed of water, fluorescein and a fluorescent auxiliary agent, and the fluorescein can fluorescently mark cells and pathogenic microorganisms in vaginal secretion. The anti-interference fluorescent auxiliary agents are respectively added into the reagents, so that the combination rate of fluorescent dyes, cells and microorganisms can be improved, the fluorescence intensity is enhanced, the cell and microorganism contours are clear and visible, the stability of a staining solution component system is improved, and the staining solution fluorescence quenching risk is reduced. The dye liquor is simple and rapid to operate, clean in background, clear in image and easy to judge, and the accuracy of vaginitis and other gynecological leucorrhea detection items can be greatly improved through clinical use.

Description

technical field [0001] The invention belongs to the technical field of medical diagnosis, and in particular relates to a dye solution for fluorescent detection of vaginal secretion microbial cells. [0002] technical background [0003] Vaginitis is a general term for a variety of vaginal mucosal inflammatory diseases caused by different etiologies. In normal physiological state, the tissue anatomy and biochemical characteristics of the vagina are sufficient to defend against the invasion of external microorganisms. If it is destroyed, pathogenic bacteria can take advantage of the opportunity to enter and cause vaginal inflammation through various factors. [0004] Vaginitis is the most common disease in obstetrics and gynecology patients. It can be divided into specific and non-specific vaginitis. Most of them are bacterial vaginitis, fungal vaginitis, and trichomonas vaginitis caused by Gardnerella, fungi, and trichomonas infection. . Bacterial vaginosis is one of the mo...

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Problems solved by technology

[0010] (1) Traditional wet film method: smear observation after dilution with normal saline, the wet film method is convenient and economical, and can be completed within a few minutes, but the observation of clue cells requires experience, the background is complex, it is easy to miss, and the overall positive detection rate is low and no way to check for dead trichomonas

[0011] (2) Gram staining: cells and microorganisms can be colored, and the shape after staining is clearer than that of wet slides, but trichomonas will be broken, and the background is relatively complex, so it is easy to miss or misdetect

[0012] (3) Enzyme chemistry method: It can be equipped with fully automated equipment and has a high degree of automation, but there are many interference factors of enzymes, no international authoritative report support, low clinical compliance rate, high probability of false positive and false negative, and complicated operation, which takes 45 minutes. Incubate at 37°C, cold chain transport

[0014] (5) PCR method: It has the advantages of specificity, sensitivity, high yield, quickness, simplicity, good repeatability, and easy automation, etc., but the operation requirements are high, the steps are complicated, and special PCR laboratories and professional laboratory physicians are required, and the detection cost is low. high

[0015] The key to the treatment of vaginitis is to accurately determine the pathogen of vaginitis, and microscopic examination is the gold standard for the diagnosis of vaginitis, but there are relatively many limitations in the detection of wet slides or ordinary staining. However, the general fluorescent staining solution has problems such as poor dyeing effect, fast quenching of fluorescence effect after staining, and unstable storage of the staining solution for a long time, resulting in low fluorescence intensity after staining.

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