Preparation of chitosan in fungal cell wall

A chitosan and fungal cell wall technology, applied in the field of chitosan extraction, can solve the problems of high energy consumption, environmental pollution, long processing time, etc., and achieve the effects of low pollution, reduced production costs, and low cost

Inactive Publication Date: 2009-07-15
中国科学院嘉兴应用化学工程中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, in the traditional process of preparation, a large amount of strong acid and alkali and high temperature treatment are required, which takes a long time to process, consumes a lot of energy, and has great pollution to the environment

Method used

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  • Preparation of chitosan in fungal cell wall

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The slant culture (PDA medium) of Actinomucor taiwanensis preserved at 4°C was directly inoculated into a 500ml shake flask containing 100ml of fresh seed culture solution, and cultured at 30°C with shaking at 280rpm for 48h. The seed medium adopts E2 medium, and each liter of seed medium contains 40g of glucose (that is, 20g of carbon source), 3.5g of NaNH 4 HPO 4 4H 2 O (ie 0.23g nitrogen source), 7.5g K 2 HPO 4 ·3H 2 O, 3.7gKH 2 PO 4 , 0.12g MgSO 4 , 0.56g CaCl 2 After 2 days of shake flask culture, the original seed was obtained, and the original seed was inoculated in a 5L fermenter.

[0022] Fill the fermenter with 40% of the liquid content, use 10 times the amount of seed medium content per liter of fermentation liquid, inoculate with 5% of the inoculum, control the temperature at 25-30°C, and the rotation speed is 100-400rpm. 6d.

[0023] The cell mass was recovered from the fermentation broth, gauze filter or centrifuged, the cells were treated with 1...

Embodiment 2

[0026] The slant culture of Actinomucor taiwanensis preserved at 4°C was directly inoculated into a 500ml shake flask containing 100ml of fresh seed culture solution. Carry out shaking flask culture as described in Example 1, the difference is that every liter of seed medium contains 20g carbon source (beef extract), 0.23g nitrogen source (3.5g NaNH 4 HPO 4 4H 2 O), 7.5g K 2 HPO 4 ·3H 2 O, 3.7g KH 2 PO 4 , 0.12g MgSO 4 , 0.56g CaCl 2 . After shaking the flask for 2 days, the original seed was obtained in the shake flask, and the original seed was used to inoculate the culture medium of the fermenter.

[0027] The amount of liquid in the fermenter is operated according to the amount of liquid in Example 1, and the amount of liquid in the fermenter is filled by 40%, and 5 times the amount of seed culture medium is used in the middle of every liter of fermented liquid. Inoculate with 5% inoculum amount, control the temperature at 25-30° C., rotate at 100-400 rpm, and fe...

Embodiment 3

[0030] Shake flask culture was carried out as described in Example 1, and cultured to a suitable concentration, generally when the dry weight of the obtained mycelium reached between 10.5 mg / ml and 15.0 mg / ml, it was used as a fermenter seed.

[0031]The amount of liquid in the fermentation tank was operated according to the amount of liquid in Example 1, except that yeast extract was used as a carbon source in the fermentation liquid, and the amount of yeast extract added was 25.0 g / L. The product was separated and extracted according to the method of Example 2, and the obtained dry cell weight was 7.1g / L to obtain chitosan 0.32g / L. The yield was 4.5%.

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Abstract

The invention relates to the culture of fungi and a preparing method of separating and extracting chitosan from mycelium. Actinomucor taiwanensis fungi are used as raw materials. After plate inscription, shaking culture and culture in fermentor, bacterial liquid is obtained. Then, the centrifugation is carried out on the obtained bacterial liquid to obtain wet cells. After separating, drying, crushing, alkali treatment, acid treatment and drying, the chitosan is obtained. The cell culturing method is simple; the process for preparing the chitosan is easy, the alkali treatment with low concentration during the deacetylation process greatly reduces pollutions on the environment, the production cost is low, and the yield is stable.

Description

technical field [0001] The invention belongs to the field of biochemical industry and relates to a method for extracting chitosan from fungal cultures of Mucoraceae. Background technique [0002] Chitosan (chitosan), also known as chitosan, deacetylated chitin, polyglucosamine, is the deacetylation product of chitin, also known as chitin, and is D-glucosamine β-1~4 Combined linear polysaccharide, the only alkaline polysaccharide among natural polysaccharides. The distribution of chitosan in nature is much less than that of chitin, and it only exists in a small amount in the cell walls of zygomycetes. Toxic, insoluble in water and alkaline solutions, soluble in most dilute acids such as hydrochloric acid, acetic acid, citric acid, etc. [0003] The main commercial value of chitosan is as a raw material for the production of glucosamine and chitosan. Chitosan has many unique physical and chemical properties and biological functions, and is widely used in many fields such as...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C12R1/645
Inventor 张利涛徐君张杰常东亮
Owner 中国科学院嘉兴应用化学工程中心
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