A kind of trichoderma aurantia and its application
A technology of Trichoderma citrus viridans and fungal cell walls, applied in the field of microorganisms, can solve the problems of environmental impact of pesticides, excessive chemical pesticides, etc., and achieve the effect of protecting the environment and reducing pepper damping-off
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[0042] The invention will be explained in more detail by means of the following examples. The following examples are illustrative only, and it should be understood that the present invention is not limited by these examples.
[0043] strain screening
[0044] Weigh 1g of soil sample into 10ml of water, mix it on a vortex instrument, and then carry out gradient dilution to 10 -5 , take 1ml and add it to the surface of the Trichoderma selective medium TSM medium, spread evenly, and culture the medium statically at 25° C. for 5-7 days. According to the shape, color and size of the fungal colonies screened on the plate, Trichoderma colonies were selected and transferred to PDA medium for purification, cultivation and further identification.
[0045] TSM medium formula (unit is g / l): MgSO 4 ·7H 2 O0.2g, KH 2 PO 4 0.9g, KCl0.15g, NH 4 NO 3 1.0g, glucose 3g, chloramphenicol 0.25g, methylsulfonate 0.3g, pentachloronitrobenzene 0.2g, rose bengal 0.15g, agar 20g.
[0046] Strai...
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