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Trichoderma citrinoviride JS84 effective for promoting crop growth and developed bio-organic fertilizer thereof

A technology of bio-organic fertilizer and Trichoderma citrus viride, applied in the field of agricultural microorganisms, can solve problems such as growth-promoting effects that have not yet been seen, and achieve the effects of obvious growth-promoting effect, stable growth-promoting ability, and increased growth speed

Active Publication Date: 2019-09-13
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] It is disclosed in many documents (CN101637187A, CN105219650A) that Trichodermacitrinoviride can prevent and treat plant fungal diseases, but there is no relevant report on its growth-promoting effect

Method used

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  • Trichoderma citrinoviride JS84 effective for promoting crop growth and developed bio-organic fertilizer thereof
  • Trichoderma citrinoviride JS84 effective for promoting crop growth and developed bio-organic fertilizer thereof
  • Trichoderma citrinoviride JS84 effective for promoting crop growth and developed bio-organic fertilizer thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1, isolation and identification of functional strains

[0023] Put 5g of soil sample in a Erlenmeyer flask filled with 45ml of sterile water, shake at 28°C for 30min (170r / min) to make a soil suspension. Take 0.1mL of soil suspension and place it on a Trichoderma selective plate and spread it, place it in an incubator at 28°C for 48 hours, observe the growth of colonies on the plate, select different colonies for purification, and inoculate the purified strains on PDA medium and stored in a refrigerator at 4°C until use.

[0024] The selected Trichoderma strains were inoculated on PDA medium, placed in 28°C, 37°C and 40°C incubators respectively, observed after 5 days of cultivation, and the high-temperature Trichoderma that could grow normally at 40°C was screened out.

[0025] Finally, a Trichoderma strain was obtained through screening and named JS84. The bacterial strain JS84 grew faster on the PDA plate. The strain JS84 grew rapidly on the PDA plate. It...

Embodiment 2

[0026] Embodiment 2, the generation of functional bacteria biological fertilizer

[0027] Inoculate bacterial strain JS84 (CGMCC No.17466) into PDA culture medium for liquid fermentation. The conditions for fermentation production are: the fermentation temperature is 28°C, the stirring speed is 170 rpm, and the fermentation time is 96h. ≥1×10 6 individual / mL. Mix 100g of straw per dry weight with 200ml of water, add to 6.6ml of acid hydrolyzed amino acid solution (the liquid amino acid complex disclosed in ZL201410042218.3) and mix well. Sterilize at 115°C for 1h. After sterilization and cooling, add 20% Trichoderma fermentation broth, mix well, spread evenly in the turnover box, cultivate at 28°C for 4-5 days, make Trichoderma strains, and store in a refrigerator at 4°C. By adding 5% to 15% of Trichoderma strains into the organic fertilizer, the Trichoderma bio-organic fertilizer is produced.

[0028] The preparation method of the PDA culture medium used is as follows, ta...

Embodiment 3

[0029] Embodiment 3, potted plant growth-promoting effect test

[0030] 3.1 Application effect of growth-promoting biofertilizer on cucumbers (first and second seasons)

[0031] The growth-promoting pot experiment was carried out in the greenhouse of the National Organic Fertilizer Engineering Technology Research Center (Yixing). The test soil was healthy sandy soil without pathogenic bacteria, and the tested cucumber variety was Lufeng cucumber without pathogenic bacteria. Soak cucumber seeds in normal temperature water for 1 hour, place them on moist gauze, and germinate them in a 30°C incubator for 12-24 hours. After more than 75% of the seeds have germinated, select the dewy seeds and move them to the seedling tray for seedling cultivation. After leaves, select seedlings with consistent growth and transplant them into pots. Two treatments were set up in the experiment: 1) CK without any fertilizer application; 2) OF treatment with ordinary organic fertilizer; 3) JS84 trea...

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Abstract

The present invention discloses a trichoderma citrinoviride JS84 effective for promoting crop growth and a developed bio-organic fertilizer thereof. The trichoderma citrinoviride JS84 is preserved inChina general microbiological culture collection center and has a preservation number of CGMCC NO.17466. The strain is capable of antagonizing various fusarium oxysporum of bananas, cucumbers, etc. The bio-organic fertilizer is obtained by adding the functional strain JS84 to an ordinary organic fertilizer and the number of viable micro-organisms is preferably larger than or equal to 2.0x10<7> CFU / g dry weight of the organic fertilizer. The product has a good growth-promoting effect in a cucumber seedling stage, can produce high-quality seedlings with a large number of active functional microorganisms in rhizosphere, and promotes growth and yield after seedling transplanting.

Description

technical field [0001] The invention belongs to the field of agricultural microorganisms, and in particular relates to a Trichoderma auranthoderma JS84 which can effectively promote crop growth and its application. Background technique [0002] Plant Growth-promoting Rhizobacteria (PGPR) refers to microorganisms that exist on the surface of the plant rhizosphere and in the soil microenvironment around the root system, and can significantly promote plant growth through direct or indirect ways (Ahmad et al., Microbiological Research, 2008, 163(2):173-181). As a kind of PGPR, Trichoderma can effectively promote plant growth, and has the characteristics of low cost, safe use, good continuous effect, stable increase in production, and safety to the environment and food. Therefore, exploring the effects of Trichoderma on crop yield and growth traits can provide a scientific basis for the rational use of Trichoderma preparations in the production of green and pollution-free vegeta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P21/00C05F11/08C12R1/885
CPCC05F11/08C12R2001/885C12N1/145A01N63/30
Inventor 沈其荣沈宗专朱佳芯李荣薛超商美妮
Owner NANJING AGRICULTURAL UNIVERSITY
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