Compound bacterial manure used for prevention and control of cotton verticillium wilt and the preparation method thereof

A technology of compound microbial bacteria and cotton verticillium wilt is applied in the directions of microorganism-based methods, biochemical equipment and methods, and botanical equipment and methods, which can solve the problem that a single antagonistic strain cannot achieve the control effect and so on.

Inactive Publication Date: 2017-01-11
FOSHAN YANHUI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] Due to the diversity and synchronous evolution of pathogenic bacteria, a single antagonistic bacterial strain cannot achieve an ideal control effect. T...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] A compound microbial bacterial fertilizer for preventing and treating Verticillium wilt of cotton is characterized in that the raw material ratio of the microbial bacterial fertilizer is: 20 parts of Streptomyces abrabraicus bacteria powder, 20 parts of Streptomyces abrane bacteria powder, 20 parts of Brevibacillus soil bacteria powder , 20 parts of bacillus clausii bacterial powder, 20 parts of trichoderma auranthoderma powder, 20 parts of potassium fulvic acid; Its preparation method comprises the following steps:

[0053] Step 1, the preparation of Streptomyces abrabrais bacteria powder

[0054] Take out the preservation tube of Streptomyces abrabrasica strains, use Gao's No. 1 solid medium to scratch the plate for recovery, and culture at 30°C for 7 days. Pick a single colony under the plate and inoculate it into an eggplant bottle with 150 ml of Gaoshi No. 1 solid medium, and culture it in an incubator at 30°C for 6-8 days. When the lawn is overgrown with the eggpl...

Embodiment 2

[0085] Example 2 Control effect test of compound microbial bacterial fertilizer on cotton verticillium wilt

[0086] (1) Test treatment:

[0087] (1) Test group one: the compound microbial bacterial fertilizer prepared in embodiment 1, the consumption of seed dressing is that every kilogram of seed is evenly dressed with 100 grams of compound microbial bacterial fertilizer; Normal cultivation after sowing. When the cotton seedlings grow to two leaves and one new true leaf, cut the roots and inoculate the spore suspension (10 8 spores / ml);

[0088] (2) Experimental group 2: No seed dressing, normal cultivation after direct sowing. When the cotton seedlings grow to two leaves and one new true leaf, cut the roots and inoculate the spore suspension (10 8 spores / ml), the compound microbial bacterial powder prepared by embodiment 1 is diluted 150 times, and every cotton root watering compound microbial bacterial powder dilution is 60 milliliters;

[0089] (3) Test group three: b...

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PUM

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Abstract

The invention discloses a compound bacterial manure used for prevention and control of cotton verticillium wilt and the preparation method thereof. Materials of the compound bacterial manure consist of Streptomyces aburaviensis, Streptomyces tanashiensis, Brevibacillus agri, Bacillus clausii, Trichoderma citrinoviride, and potassium fulvic acid. Bacteria of the invention can make full use of the interspecific or intraspecific antibiosis, competition, hyperparasite, and bacteriolysis of microbes, and induce cotton to generate the disease resistance ability through secondary metabolite, thereby producing various antibiotic substances such as luteomycin and aburamycin to prevent the pathogenic bacteria of cotton verticillium wilt. The 5 strains used in the invention can be obtained through separation from soil directly; the strains can promote the growth of root, and secrete some secondary metabolites to plant roots while growing on the plant surface, inside the plant or in soil, thereby improving the plant's absorption of nutrients, and promoting the plant growth like fertilizers.

Description

technical field [0001] The invention belongs to the technical field of agricultural biological control and microbial fertilizer, and in particular relates to a compound microbial fertilizer for preventing and controlling cotton verticillium wilt and a preparation method thereof. Background technique [0002] Cotton Verticillium dahliae is a soil-borne disease that is caused by Verticillium dahaliae and is difficult to control. Cotton verticillium wilt frequently breaks out in my country, and the cotton planting area affected by it is more than 40 million mu every year, causing a reduction in cotton production by about 10%-20% all the year round, and the production reduction can be as high as 60%- 70%. [0003] The methods of preventing and treating Verticillium wilt of cotton mainly include: one is chemical control, but the effect of chemical control is relatively poor, and it is easy to cause environmental pollution and destroy the ecological balance of farmland; the other ...

Claims

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Application Information

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IPC IPC(8): A01N63/04A01N63/00A01P3/00C05G3/00C12N1/20C12N1/14C12R1/465C12R1/13C12R1/07C12R1/885
CPCA01N63/00A01N63/30C05F11/02C05G3/00C12N1/14C12N1/20A01N2300/00C05F11/08
Inventor 蒋常德胡艳晖
Owner FOSHAN YANHUI BIOTECH CO LTD
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