Strain capable of producing 1,4-beta-D-xylanase and application of strain

Abstract

The invention relates to a strain capable of producing 1,4-beta-D-xylanase and an application of the strain and can effectively solve the problem about production of 1,4-beta-D-xylanase to meet demands in industry. The method comprises steps as follows: a culture medium for enzyme production by fermentation is inoculated with the strain capable of producing 1,4-beta-D-xylanase, a fermentation broth of 1,4-beta-D-xylanase is obtained and centrifuged, ammonium sulfate is slowly dropwise added to a liquid supernatant and salted out overnight, a salting-out liquid is centrifuged, precipitates are taken and dissolved with a buffer solution, packing is performed, a buffer solution is taken as a balance solution for DAE FF gel filtration chromatography, active components are collected, packing is performed again, linear elution is performed with a balance buffer solution, active components are collected, the mass concentration of ammonium sulfate in the active components is adjusted to 40%, packing is performed, an ammonia sulfate buffer solution is taken as a balance solution for linear elution, active components are collected, salt is removed through dialysis, and dry powder of 1,4-beta-D-xylanase is obtained through freezing and suction drying. The strain capable of producing 1,4-beta-D-xylanase is a novel strain Trichoderma citrinoviride HL28-5 and has the efficacy of producing 1,4-beta-D-xylanase, and a new way of producing 1,4-beta-D-xylanase is exploited.

Description

technical field [0001] The invention relates to a biological enzyme, in particular to a strain producing xylanase and its application. Background technique [0002] Xylanase (1,4-β-D-xylanase: EC3.2.1.8) is an important industrial enzyme that plays a biocatalytic role in decomposing xylan, which can decompose xylan into polysaccharides or Monosaccharide protein or RNA. Xylanases are widely found in organisms in nature. Bacteria, fungi, animals, etc. can produce xylanase. [0003] Xylanases include endo1.3-β-xylanase (EC3.2.1.32), endo1.4-β-xylanase (EC3.2.1.8), xylan1.3-β-xyloside enzyme (EC3.2.1.72), xylan 1.4-β-xylosidase (EC3.2.1.37) and acetylxylan esterase (EC3.1.1.6). Endo-xylanase hydrolyzes xylan to generate xylooligosaccharides and xylose, and exo-xylanase hydrolyzes xylooligosaccharides, cutting off xylose residues from the non-reducing end. Xylanase (1,4-β-D-xylanase; EC 3.2.1.8) is an important industrial enzyme, which has important application value in pape...

AI Technical Summary

Problems solved by technology

However, the optimum reaction pH of most xylanases is between 4.0 and 6.0, and the narrow optimum reaction pH limits the industrial application of xylanases.

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