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Trichoderma citrinoviride M-13 and application thereof

A technology of Trichoderma tangerineum, M-13, applied in other chemical processes, water/sludge/sewage treatment, biochemical equipment and methods, etc. The effect of good application prospect, high processing efficiency and simple process

Inactive Publication Date: 2014-04-23
SHANGHAI OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods have shortcomings such as incomplete removal, cumbersome operation, high operating cost, high cost, and secondary pollution.

Method used

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  • Trichoderma citrinoviride M-13 and application thereof
  • Trichoderma citrinoviride M-13 and application thereof
  • Trichoderma citrinoviride M-13 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] strain source

[0019] An abandoned steel factory in Shanghai is located in a subtropical monsoon climate zone. Soil samples were taken from an abandoned steel factory in Shanghai in September 2012 and put into sterile bags.

[0020] Media and Reagents

[0021] Potassium dichromate (excellent grade), dry at 110°C for 2 hours before use. The reagents used in the medium (Bengal red medium, Martin medium) were purchased from Sinopharm; Taq DNA polymerase and dNTP were purchased from Dalian TaKaRa Company; DNA Marker was purchased from Beijing Tiangen Biochemical Technology Co., Ltd.; antibiotics were purchased from the UK OXOID; Milli-Q water.

[0022] Initial isolation of mold strains

[0023] Weigh 25g from the collected soil sample, dissolve it into a sampling cup (sterilized) filled with an appropriate amount of glass beads and 225mL sterile water, shake well, and dilute with 0.9% sterile normal saline gradiently to 10 -1 、10 -2 、10 -3 、10 -4 . Spread on Benga...

Embodiment 2

[0025] Example 2 Resistance to Cr 6+ Mold reduction of hexavalent chromium ions

[0026] The strain M-13 with high tolerance to slant preservation was inoculated in the non-added Cr 6+ Cultivate in liquid culture medium for 72 hours, collect mycelia, dry naturally for 4 hours, weigh 1.0 g of mycelium and place in 50ml100mg / L Cr 6+ In aqueous solution, culture at 28°C, 150r / min shaking for 48h, and filter. Measure the content of chromium ion in its filtrate with ICP-MS, repeat 3 times, calculate the average value, with no inoculation but containing 100mg / L Cr 6+ aqueous solution as a control. The strain with the best treatment effect was selected, named as M-13, and further identified.

[0027] ICP-MS detection is carried out according to the method described in the following literature:

[0028] (1) Pitt JI. 1979. The genus Penicillium and its teleomorphic states Eupenicillium and Talaromyces. London: Academic Press.

[0029] (2) Determination of hexavalent chromium in N...

Embodiment 3

[0033] Example 3 Reduction of Cr 6+ Identification of mold

[0034] 1. Molecular Systematic Identification

[0035] will be screened to highly adsorbed Cr-resistant 6+The mold was inoculated on the medium plate and cultured for 48 hours. Take a single colony and culture it on a shaker in a liquid medium for 48 hours, and use quartz sand to grind the CTAB extraction method (Scott et al., 2000, DNA heteroduplex fingerprinting in Penicillium. In: Samson RA, Pitt JI (eds). Integration of modern taxonomic methods for Penicillium and Aspergillus classification.Amsterdam: Harwood Academic Publishers.p.225–236), the DNA of the extracted strain was used as a template for PCR amplification. The translation elongation factor (tef1-alpha) gene (tef) was selected, and the amplification primers were EF1T and EF2T (Bischoff et al., 2006. Metarhizium frigidum sp.nov.: a cryptic species of M. anisopliae and a member of the M. flavoviride complex. Mycologia, 98:737–745).

[0036] PCR ampli...

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Abstract

The present invention relates to the field of microorganisms and water treatments, and discloses Trichoderma citrinoviride M-13 with the preservation number of CGMCC No.8332, wherein the microorganism can be used for reducing or absorbing Cr<6+> and treating the water body containing Cr<6+>, provides a restoration effect for the contaminated water body, and can be used for sewage treatments. According to the invention, the microorganism is used for sewage treatments, and has characteristics of simple process, high treatment efficiency, low cost and good application prospects.

Description

technical field [0001] The invention relates to the field of microorganisms and water treatment, in particular to a method that can reduce Cr in water bodies 6+ of Trichoderma citrus viride. Background technique [0002] At present, heavy metals pollute water bodies more and more seriously, and the concentration and types of heavy metals in water bodies increase greatly. Some metals are not only expensive, but also extremely harmful to the ecological environment and human health. Therefore, how to effectively treat heavy metals in wastewater has become more and more important and urgent. [0003] Chromium widely exists in nature, such as rocks, soil, plants, animals and volcanoes. Chromium pollution is mainly caused by wastewater from electroplating, tanning, metallurgy and other production processes. Chromium ions mainly exist in the form of trivalent cations and hexavalent anions. Cr(VI) is soluble, highly toxic and carcinogenic; Cr(III) is slightly soluble and less to...

Claims

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Application Information

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IPC IPC(8): C12N1/14B01J20/22C02F3/34C02F1/28C12R1/885C02F101/22
Inventor 马晨晨欧杰殷曦敏董博张皓文
Owner SHANGHAI OCEAN UNIV
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