Application of penicillium enol E1 deriving from trichoderma citrinoviride in preparation of anti-lymphoma drug
A technology of penemalol and Trichoderma aurantii, which is applied in the field of medicinal chemistry, can solve the problems of no drugs and achieve significant anti-lymphoma activity
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Embodiment 1
[0015] Fermentative production and separation and purification of the compound of embodiment 1
[0016] 1 Fermentation production
[0017] Fermentation culture of production bacteria: according to the conventional method of cultivating microorganisms, take Trichoderma auranthoderma ( Trichoderma citrinoviride. ) IBPT-4 (preserved in China Center for Type Culture Collection on January 25, 2013, address: Wuhan University, Wuhan, deposit number is: CCTCC NO: M 2013055) appropriate amount, inoculated on PDA solid slant medium at 28 Cultivate in an incubator for 4 days.
[0018] Get the Trichoderma auranthoderma ( Trichoderma citrinoviride. ) appropriate amount of IBPT-4, inoculated into 400mL culture medium [Culture medium composition (g / L): mannitol 20.0, yeast extract 3.0, maltose 20.0, monosodium glutamate 10.0, glucose 10.0, KH 2 PO 4 0.5 , MgSO 4 0.3, NaCl 6.0, fixed volume into 1000mL Erlenmeyer flask], after static culture at 28°C for 30 days, mycelia and fermenta...
Embodiment 2
[0027] Example 2 Test of anti-tumor activity in vitro
[0028] 1 Experimental samples and experimental methods
[0029] Preparation of the tested sample solution The test sample is the pure product of the compound separated and refined in the above-mentioned implementation 1. Accurately weigh an appropriate amount of sample, and prepare a solution with the required concentration with methanol for activity measurement.
[0030] Cell lines and subculture of cells Lymphoma cell lines were used, and cells were subcultured in RPMI-1640 medium containing 10% FBS at 37°C in an incubator filled with 5% carbon dioxide.
[0031] Cell Proliferation Inhibitory Activity Test Method
[0032] Tetrazolium salt (MTT) method Take cells in the logarithmic growth phase and adjust the cell density to 2×10 per ml 5 Cells were inoculated into 96-well cell culture plates at 200 microliters per well, and filled with 5% CO at 37°C 2 for 4 hours in an incubator. Add 2 microliters of sample solution...
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