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Trichoderma citrinoviride and application thereof

A technology of Trichoderma citrus viridans and its application, applied in the field of microorganisms, Trichoderma citrus viridans and its biological control of plant diseases, can solve the problems of chemical pesticide environmental pollution and other problems

Inactive Publication Date: 2015-11-11
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Pathogens are a major "killer" that affects plant growth and development and leads to crop yield reduction. Since the invention of Bordeaux mixture, chemical prevention and control has been the most important measure to control the occurrence, prevalence, damage, and disaster of plant diseases; Pesticide application increases the risk of environmental pollution caused by chemical pesticides. Therefore, the biological control of diseases by using the interaction between biological species has become the development direction of plant disease control and ecological environment protection.

Method used

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  • Trichoderma citrinoviride and application thereof
  • Trichoderma citrinoviride and application thereof
  • Trichoderma citrinoviride and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Isolation, purification, identification and preservation of the bacterial strain of embodiment 1

[0037] 1. Single spore isolation and purification of Trichoderma strains

[0038] Drop 75% ethanol and sterile water into the groove of a sterile cuvette, pick a mature subsea and place it in the ethanol in the cuvette for surface disinfection, rinse with sterile water, and rinse with sterile water. Crush the ascus with tweezers, pick the ascus shell containing the ascus into another cuvette, further crush the ascus, stir well, spread the suspension evenly on the water agar medium, and incubate at room temperature. Place them under a dissecting microscope for observation every other day, pick out germinated single spores with a sterile dissecting needle, transfer them to PDA medium containing ampicillin (2mg / L), and place them in dark culture at 25°C until the growth of Trichoderma After the ascospores germinate and colonies are formed, transfer the actively growing part ...

Embodiment 2

[0056] Embodiment 2 confrontation culture method

[0057] Trichoderma aeruginosa P-T7987 and five target plant pathogens (Botrytis cinerea, Sclerotinia sclerotiorum, Pythium melonum, Phytophthora capsici, Rhizoctonia solani) were activated and cultured on PDA medium, and then the Take a bacterial block with a diameter of 5 mm, and inoculate Trichoderma aurantii P-T7987 and phytopathogens at symmetrical positions 15 mm away from the edge of the dish on both sides on the diameter line, and at the same time, only inoculate phytopathogens as a blank control, and set 3 times for each treatment Repeat, culture at a constant temperature of 25°C, and observe the growth of the strain daily. When the petri dish is covered with the control, measure the control growth amount (colony radius) and the treatment growth amount (inhibition growth radius after inoculation of Trichoderma) of the target bacteria, and calculate the bacteriostatic rate.

[0058] Bacterial inhibition rate (%)=(contr...

Embodiment 3

[0062] Example 3 Heavy Parasitic Observation

[0063] Using the glass slide culture method, use a sterilized scalpel to cut out a 15×10mm PDA film, place it in the center of a sterilized glass slide, and then pick out an equal amount of hyphae of Trichoderma aurantii P-T7987 and plant pathogen They were respectively inoculated at the midpoint of the two parallel sides of the PDA film, cultured at a constant temperature and humidity at 25°C, and the interaction between the two bacteria was observed daily under the microscope.

[0064] The reparasitic process of Trichoderma aeruginosa P-T7987 on plant pathogens was observed by glass slide culture method combined with optical microscope. The hyphae of Trichoderma aurantii P-T7987 can recognize and tend to grow five plant pathogens, and The adsorption structure of mycelial tendrils attaches and gradually wraps around the mycelium of plant pathogens, and then dissolves the host cell wall through the secretion of various enzymes and...

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Abstract

The invention relates to a trichoderma citrinoviride P-T 7987 strain, which is preserved in China General Microbiological Culture Collection Center and is assigned the accession number CGMCC No.10646. The trichoderma citrinoviride P-T 7987 is strong in habitat adaptability, is high in growth speed, is high in sporulation quantity, can be used for inhibiting botrytis cinema, sclerotinia sclerotiorum, pythium aphanidermatum, phytophthora capsici and rhizoctonia solani, can be hyper-parasitized on hypha of the botrytis cinema, the sclerotinia sclerotiorum, the pythium aphanidermatum, the phytophthora capsici and the rhizoctonia solani to rapidly and effectively degrade the vegetable bacteria. The trichoderma citrinoviride P-T 7987 has a potential and developable biological prevention and treatment value.

Description

technical field [0001] The invention belongs to the field of microorganisms, and more specifically relates to a strain of Trichoderma aurantii and its use in the field of biological control of plant diseases. Background technique [0002] Pathogens are a major "killer" that affects plant growth and development and leads to crop yield reduction. Since the invention of Bordeaux mixture, chemical prevention and control has been the most important measure to control the occurrence, prevalence, damage, and disaster of plant diseases; Pesticide application increases the risk of environmental pollution caused by chemical pesticides. Therefore, the biological control of diseases by using the interaction between biological species has become the development direction of plant disease control and ecological environment protection. [0003] Since Weindling first discovered in 1932 that Trichodermalignorum can parasitize certain plant pathogens and reduce their damage, related research ...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/885
Inventor 庄文颖秦文韬朱兆香宋霞
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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