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Microbial inoculum for preventing and curing fungal diseases of plants

A technology of plant fungal diseases and fungicides, applied in the direction of plant growth regulators, fungicides, biocides, etc., can solve problems such as increased chemical pesticide consumption, drug resistance or drug resistance of pathogenic bacteria, and environmental pollution

Active Publication Date: 2013-06-19
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, long-term, repeated and large-scale use of chemical pesticides will lead to the killing or inhibition of beneficial microorganisms, destroy the structure of biological communities, cause environmental pollution, and cause a large number of harmful substances in vegetables to remain, leading to the development of drug resistance or drug resistance of pathogenic bacteria, thus making chemical pesticides The increased use of the

Method used

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  • Microbial inoculum for preventing and curing fungal diseases of plants
  • Microbial inoculum for preventing and curing fungal diseases of plants
  • Microbial inoculum for preventing and curing fungal diseases of plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1, the preparation of the bacterial agent of prevention and treatment plant fungus disease and its control effect experiment

[0020] 1. Preparation of liquid inoculant for preventing and controlling plant fungal diseases

[0021] Screening medium for chlamydospore production: KH 2 PO 4 2.0g / L, NH 4 NO 3 1.0g / L, FeSO 4· 7H 2 O0.005g / L, MnSO 4 0.0016g / L, CaCl 2 0.3g / L, NaCl1.0g / L, MgSO 4· 7H 2 O0.3g / L, corn stalk powder (over 40mm sieve) 10g / L. PH6.0. Sterilize at 115°C for 20 minutes.

[0022] Inoculate Trichoderma longifolia ACCC30150 and Trichoderma limegreen ACCC30152 into PDA liquid medium respectively, and culture on a shaker at 30° C. for 3 days (the shaker rotates at 265 rpm). Then according to the inoculum size of 10% (volume percentage composition), inoculate in the above-mentioned screening and produce chlamydospore culture medium, 30 ℃ of shaking table cultures (shaking table rotating speed 250 rev / mins) to the growth of Trichoderma long...

Embodiment 2

[0070] Embodiment 2, the preparation of the bacterial agent of prevention and treatment plant fungus disease and control effect experiment thereof

[0071] 1. Preparation of liquid inoculant for preventing and controlling plant fungal diseases

[0072] Screening medium for chlamydospore production: KH 2 PO 4 2.0g / L, NH 4 NO 3 1.0g / L, FeSO 4 .7H 2 O0.005g / L, MnSO 4 0.0016g / L, CaCl 2 0.3g / L, NaCl1.0g / L, MgSO 4· 7H 2 O0.3g / L, corn stalk powder (over 40mm sieve) 10g / L. PH6.0. Sterilize at 115°C for 20 minutes.

[0073] Inoculate Trichoderma longifolia ACCC30150 and Trichoderma limegreen ACCC30152 into PDA liquid medium respectively, and culture on a shaker at 30° C. for 3 days (the shaker rotates at 265 rpm). Then according to the inoculum size of 10% (volume percentage composition), inoculate in the above-mentioned screening and produce chlamydospore culture medium, 30 ℃ of shaking table cultures (shaking table rotating speed 250 rev / mins) to the growth of Trichoderma ...

Embodiment 3

[0115] Embodiment 3, the preparation of the bacterial agent of preventing and treating plant fungus disease and its control effect experiment

[0116] 1. Preparation of liquid inoculant for preventing and controlling plant fungal diseases

[0117] Screening medium for chlamydospore production: KH 2 PO 4 2.0g / L, NH 4 NO 3 1.0g / L, FeSO 4· 7H 2 O0.005g / L, MnSO 4 0.0016g / L, CaCl 2 0.3g / L, NaCl1.0g / L, MgSO 4· 7H 2 O0.3g / L, corn stalk powder (over 40mm sieve) 10g / L. PH6.0. Sterilize at 115°C for 20 minutes.

[0118] Inoculate Trichoderma longifolia ACCC30150 and Trichoderma limegreen ACCC30152 into PDA liquid medium respectively, and culture on a shaker at 30° C. for 3 days (the shaker rotates at 265 rpm). Then according to the inoculum size of 10% (volume percentage composition), inoculate in the above-mentioned screening and produce chlamydospore culture medium, 30 ℃ of shaking table cultures (shaking table rotating speed 250 rev / mins) to the growth of Trichoderma longh...

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Abstract

The invention discloses a microbial inoculum for preventing and curing fungal diseases of plants; the active ingredients contain Trichoderma longibrachiatum and Trichoderma citrinoviride. The microbial inoculum of the invention is used to carry out control efficiency experiments and the results show that the control efficiency of liquid microbial inoculum to cucumber blight is up to 54.8% and thecontrol efficiency to green pepper phytophthora disease is up to 67.9%; the control efficiency of solid microbial inoculum to cucumber blight is up to 45.12% and the yield of cucumber is increased by11.06% so that the effect for preventing diseases and increasing yield is obvious; the control efficiency of solid microbial inoculum to green pepper phytophthora disease is up to 61.90% and the average plant height of the green pepper in experimental group is increased by 8.92% compared with that of the green pepper in control group and the yield of green pepper is increased by 37.19% so that the microbial inoculum of the invention is shown to have obvious effect for preventing increases, promoting the growth and increasing the yield.

Description

technical field [0001] The invention relates to a bacterial agent for preventing and treating fungal diseases of plants. Background technique [0002] Since the 1980s, my country's vegetable planting industry has developed rapidly, and vegetables have become the second largest crop after food crops. Due to the special environment of vegetable cultivation, suitable conditions are provided for the occurrence and development of vegetable diseases, resulting in an increasing trend in the types, quantity and degree of damage of vegetable diseases year by year, seriously affecting the yield and quality of vegetables, and causing great harm to vegetable farmers. The huge economic loss has become a "bottleneck" that restricts the development of my country's vegetable planting industry and enhances the competitiveness of the international market. The prevention and control of vegetable diseases is a necessity for the development of vegetable planting industry in my country, and it is...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/04A01P3/00
Inventor 李世贵顾金刚姜瑞波张晓霞张瑞颖阮志勇马晓彤
Owner INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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