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Trichoderma citrinoviride and application thereof in degradation of fish protein

A technology of Trichoderma aurantiacum and fish protein, which is applied in the field of microorganisms, can solve problems such as unsuitability for use, and achieve the effect of being easy to promote and use

Active Publication Date: 2022-06-03
SHANDONG FOREST SCI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

CN202111306622.3 discloses a method for preparing soft-shelled turtle biologically active peptides. The soft-shelled turtle protein is fermented by using a combination of Aspergillus cinnamon and Lactococcus lactis subsp. Aspergillus cinnamon and Lactococcus lactis subspecies lactic acid strains can produce a variety of different proteases, so that the soft-shelled turtle protein is decomposed into a variety of different small molecule peptide chains. Although the method uses bacterial strains to degrade the soft-shelled turtle protein, However, it is not suitable for use in fertilizers for the preparation of foods based on active polypeptides

Method used

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  • Trichoderma citrinoviride and application thereof in degradation of fish protein
  • Trichoderma citrinoviride and application thereof in degradation of fish protein
  • Trichoderma citrinoviride and application thereof in degradation of fish protein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: Isolation and identification of strains

[0023] 1. Isolation of strain 0-1

[0024] The Trichoderma viridis strain 0-1 of the present invention is collected from the bark of Pinus massoniana in Changdao Pinus massoniana forest farm in Yantai, Shandong Province, and is separated and obtained by the dilution plate method. The specific method is as follows:

[0025] 1.1 Sample processing:

[0026] Masson pine bark was collected from the Masson pine forest farm in Yantai Changdao, Shandong Province, the collected samples were washed with distilled water, weighed and cut into 1mm 2 soaked in 75% ethanol solution for 40-60s, then soaked in 0.1% mercuric solution for about 80s, and then soaked in 75% ethanol for 30s; after taking out, rinse with sterile water for 4- 6 times;

[0027] 1.2 Bacteria sample acquisition:

[0028] Put the processed sample into a sterile mortar containing 10 mL of sterile water and a little sterilized quartz sand, fully grind it into...

Embodiment 2

[0056] Example 2: Preparation of Trichoderma viridis 0-1 microbial inoculum

[0057] (1) After activation, Trichoderma citrinum 0-1 was inoculated on the surface of a PDA plate, and incubated at a constant temperature of 28°C for 48h;

[0058] (2) The hole puncher punches the bacterial cake at the edge of the activated strain, inoculates it in the seed liquid medium, and cultivates in a shaking flask at 28° C. and 120 r / min for 72 h, to obtain the seed liquid of Trichoderma citrinum 0-1;

[0059] The seed liquid medium is PDB medium: 200 g of potato, 15 g of glucose (or sucrose), 1000 mL of water, and natural pH. Aliquot and sterilize at 121°C for 25min.

[0060] (3) the seed liquid prepared in step (2) is inoculated into the fermentation medium, the inoculation mass ratio is 1:50, the fermentation medium is the same as the seed liquid medium, and the fermentation conditions are the same as the step (2), to obtain Trichoderma viridis. -1 of the fermentation broth.

[0061] ...

Embodiment 3

[0062] Example 3: Functional verification of efficient degradation of fish protein by Trichoderma viridis 0-1

[0063] (1) Experimental method

[0064] Fully pulverize the fresh blue trout (Qingzhan fish) in a pulverizer to make fish puree, fully mix the fish puree with water in a mass ratio of 1:1 to make fish paste; take 200mL of fish paste into 500mL In the aseptic triangular flask, the spore suspension of Trichoderma viridis 0-1 was inserted according to the mass ratio of 5% inoculum; the control group was inserted into the fermentation medium (i.e. PDB medium) according to 5% inoculum, and each treatment Repeat for three bottles.

[0065] After the treatment was completed, it was fermented at 28 °C and 120 r / min for 48 h, and samples were sent to the Guangzhou Analysis and Testing Center in China to detect the content of free amino acids.

[0066] (2) Analysis of results

[0067] Analysis of the results showed that the total free amino acid content of samples not treat...

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Abstract

The invention discloses trichoderma citrinoviride and application thereof in degradation of fish protein. According to the invention, Trichoderma citrinoviride 0-1 with an efficient degradation effect on fish proteins is screened from masson pine barks, and the Trichoderma citrinoviride 0-1 is preserved in the China General Microbiological Culture Collection Center on August 20, 2021, and the preservation number is CGMCC NO.23215. The Trichoderma citrinoviride 0-1 can be used for degrading fish proteins. The strain not only can efficiently degrade fish protein, but also has a better inhibition effect on cotton verticillium wilt and taro fusarium wilt, and can be developed into a novel fish protein functional fertilizer taking the strain as a functional strain.

Description

technical field [0001] The invention relates to a strain of Trichoderma citrinum and its application in degrading fish protein, belonging to the technical field of microorganisms. Background technique [0002] With the rapid development of modern agriculture and green agriculture, traditional chemical fertilizers can no longer meet the requirements of agricultural production, the production efficiency of agricultural products is low, and the safety problems of agricultural products are becoming more and more prominent. Therefore, multi-functional and new fertilizers are urgently needed to replace traditional chemical fertilizers and promote modern Green agriculture moves forward. In recent years, new fertilizers have developed rapidly. Among them, fish protein fertilizer is the most popular new type of fertilizer. The various amino acids and small peptides produced by the degradation of fish protein can significantly promote the improvement of crop quality. At the same tim...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P21/00C05F17/20C05C11/00A01N63/38A01P3/00C12R1/885
CPCC12N1/14C12P21/00C05F17/20C05C11/00A01N63/38Y02W30/40
Inventor 杨庆山张淑静王静陈迪周健马安宝魏海霞王莉莉王振猛何明明孙超
Owner SHANDONG FOREST SCI RES INST
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