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Application of penemenol E1 derived from trichoderma citrinoviride in an aspect of resisting nasopharynx cancer

A technology of penemalol and Trichoderma aurantii, applied in the direction of microorganism-based methods, medical preparations containing active ingredients, pharmaceutical formulations, etc., can solve the problems of no drugs and achieve significant anti-nasopharyngeal carcinoma activity Effect

Inactive Publication Date: 2017-02-22
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The study found that the alkaloid compound shown has anti-tumor activity against nasopharyngeal carcinoma, but there is no report on the chemical structure of the compound and its anti-proliferation activity against nasopharyngeal carcinoma cells, so there is no related drug on the market

Method used

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  • Application of penemenol E1 derived from trichoderma citrinoviride in an aspect of resisting nasopharynx cancer
  • Application of penemenol E1 derived from trichoderma citrinoviride in an aspect of resisting nasopharynx cancer
  • Application of penemenol E1 derived from trichoderma citrinoviride in an aspect of resisting nasopharynx cancer

Examples

Experimental program
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Effect test

Embodiment 1

[0015] Fermentative production and separation and purification of the compound of embodiment 1

[0016] 1 Fermentation production

[0017] Fermentation culture of production bacteria: according to the conventional method of cultivating microorganisms, take Trichoderma auranthoderma ( Trichoderma citrinoviride. ) IBPT-4 (preserved in China Center for Type Culture Collection on January 25, 2013, address: Wuhan University, Wuhan, deposit number is: CCTCC NO: M 2013055) appropriate amount, inoculated on PDA solid slant medium at 28 Cultivate in an incubator for 4 days.

[0018] Get the Trichoderma auranthoderma ( Trichoderma citrinoviride. ) appropriate amount of IBPT-4, inoculated into 400mL culture medium [Culture medium composition (g / L): mannitol 20.0, yeast extract 3.0, maltose 20.0, monosodium glutamate 10.0, glucose 10.0, KH 2 PO 4 0.5 , MgSO 4 0.3, NaCL 6.0 constant volume] in a 1000mL Erlenmeyer flask, after 30 days of static culture at 28°C, the mycelia and fer...

Embodiment 2

[0027] Example 2 Test of anti-tumor activity in vitro

[0028] 1 Experimental samples and experimental methods

[0029] Preparation of the tested sample solution The test sample is the pure product of the compound separated and refined in the above-mentioned implementation 1. Accurately weigh an appropriate amount of sample, and prepare a solution with the required concentration with methanol for activity measurement.

[0030] Cell lines and cell subculture The nasopharyngeal carcinoma cell line was used, and the cells were subcultured in RPMI-1640 medium containing 10% FBS at 37°C in an incubator filled with 5% carbon dioxide.

[0031] Cell Proliferation Inhibitory Activity Test Method

[0032] Tetrazolium salt (MTT) method Take cells in the logarithmic growth phase and adjust the cell density to 2×10 per ml 5 Cells were inoculated into 96-well cell culture plates at 200 microliters per well, and filled with 5% CO at 37°C 2 for 4 hours in an incubator. Add 2 microliters ...

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Abstract

The invention relates to an application of penemenol E1 derived from trichoderma citrinoviride in an aspect of resisting nasopharynx cancer. The compound has the following structural characteristics: the compound contains the molecular skeleton of a pentacyclic amide, the compound contains a carbonyl group which is linked to a decane saturated fat long chain, a methyl group is linked to N, and two hydroxyl groups exist in molecules. Experiments prove that the alkaloid compound has a relatively good inhibitory activity on nasopharynx cancer; and the alkaloid compound can be used for preparing medicines for inhibiting proliferation of nasopharynx cancer cells or medicines for resisting the nasopharynx cancer, and the alkaloid compound is applicable to anti-tumor researches.

Description

technical field [0001] The invention relates to the application of penemenol E1 derived from Trichoderma aurantii in nasopharyngeal carcinoma. Background technique [0002] Alkaloids are a type of nitrogen-containing organic compounds produced by biological secondary metabolism. There are many types of alkaloids in nature, most of which come from plants, so they are also called plant alkaloids. Alkaloids have important physiological effects on humans and animals, including anti-asthma and antitussive, hypoglycemic, hypolipidemic, antibacterial, antitumor, analgesic, etc., among which antibacterial and antitumor activities are the most prominent. Natural structural alkaloids are an important source for discovering lead compounds in the research of innovative drugs, and there are nearly a hundred kinds of alkaloid drugs currently used in clinical practice. Studies have found that some marine fungi can produce alkaloids with novel structures and good activities in the process ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/4015A61P35/00C07D207/38C12P17/10C12R1/885
CPCA61K31/4015C07D207/38C12P17/10
Inventor 陈立李亚平刘沁颖李欣欣毕延雪赵杨杨张其清
Owner FUZHOU UNIV
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