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Application of trichoderma citrinoviride-derived penicillium enol E1 to liver cancer aspect

A technology of Trichoderma aurantii and anti-liver cancer, which is applied in the direction of medical preparations containing active ingredients, pharmaceutical formulas, organic active ingredients, etc., can solve problems such as drugs that have not yet been seen, and achieve significant anti-hepatoma activity

Inactive Publication Date: 2017-02-15
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The study found that the alkaloid compound shown has anti-tumor activity against liver cancer, but there is no report on the chemical structure of the compound and its anti-proliferation activity against liver cancer cells, so there is no related drug on the market

Method used

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  • Application of trichoderma citrinoviride-derived penicillium enol E1 to liver cancer aspect
  • Application of trichoderma citrinoviride-derived penicillium enol E1 to liver cancer aspect
  • Application of trichoderma citrinoviride-derived penicillium enol E1 to liver cancer aspect

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1 Fermentative production and separation and purification of compounds

[0016] 1. Fermentation production

[0017] Fermentation culture of production bacteria: according to the conventional method of cultivating microorganisms, take Trichoderma auranthoderma ( Trichoderma citrinoviride. ) IBPT-4 (preserved in the China Center for Type Culture Collection on January 25, 2013, address: Wuhan University, Wuhan, deposit number: CCTCC NO: M 2013055) appropriate amount, inoculated on PDA solid slant medium, in Cultivate for 4 days in a 28°C incubator.

[0018] Get the Trichoderma auranthoderma ( Trichoderma citrinoviride. ) appropriate amount of IBPT-4, inoculated into 400mL culture medium [composition of culture medium (g / L): mannitol 20.0, yeast extract 3.0, maltose 20.0, monosodium glutamate 10.0, glucose 10.0, KH 2 PO 4 0.5, MgSO 4 0.3, NaCl 6.0 constant volume] in a 1000mL Erlenmeyer flask, after 30 days of static culture at 28°C, mycelia and fermentati...

Embodiment 2

[0026] Example 2 Test of anti-tumor activity in vitro

[0027] 1. Experimental samples and experimental methods

[0028] Preparation of the test sample solution: the test sample is the pure product of the compound isolated and refined in Example 1. Accurately weigh an appropriate amount of sample, and prepare a solution with the required concentration with methanol for activity measurement.

[0029] The subculture of cell lines and cells adopts liver cancer cell lines, and the cells use RPMI-1640 medium containing 10% FBS at 37°C and 5% CO 2 Subculture in an incubator.

[0030] Cell Proliferation Inhibitory Activity Test Method

[0031] Tetrazolium salt (MTT) method: Take cells in the logarithmic growth phase and adjust the cell density to 2×10 per ml 5 Cells were inoculated in 96-well cell culture plates at 200 microliters per well, and kept at 37°C in 5% CO 2 for 4 hours in an incubator. Add 2 microliters of sample solution or blank solution to each well, and after 24 ...

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Abstract

The invention discloses application of trichoderma citrinoviride-derived penicillium enol E1 to a liver cancer aspect. The compound has the following structure characteristics: a molecular skeleton contains one pentacyclic amide, one carbonyl is connected with one 10-carbon saturated fat long chain, N is connected with one methyl and two hydroxyl groups exist in the molecule. Experiments prove that the alkaloid compound has high inhibition activity on liver cancer cells, can be used for preparing medicines for inhibiting proliferation of the liver cancer cells or medicines for resisting liver cancer, and is applied to anti-tumor research.

Description

technical field [0001] The invention relates to the application of an alkaloid compound penemenol E1 derived from Trichoderma aurantii in liver cancer. Background technique [0002] Alkaloids are a type of nitrogen-containing organic compounds produced by biological secondary metabolism. There are many types of alkaloids in nature, most of which come from plants, so they are also called plant alkaloids. Alkaloids have important physiological effects on humans and animals, including anti-asthma and antitussive, hypoglycemic, hypolipidemic, antibacterial, antitumor, analgesic, etc., among which antibacterial and antitumor activities are the most prominent. Natural structural alkaloids are an important source for discovering lead compounds in the research of innovative drugs, and there are nearly a hundred kinds of alkaloid drugs currently used in clinical practice. Studies have found that some marine fungi can produce alkaloids with novel structures and good activities in the...

Claims

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Application Information

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IPC IPC(8): A61K31/4015A61P35/00C12P17/10
CPCA61K31/4015C12P17/10
Inventor 陈立李欣欣刘沁颖李亚平毕延雪赵杨杨张其清
Owner FUZHOU UNIV
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