A kind of Tianmingjing extract and preparation method and application in anti-hepatocellular carcinoma active drug through jak2/stat3 channel

An active drug, anti-cancer technology, applied in anti-tumor drugs, drug combinations, plant/algae/fungus/moss components, etc., can solve the problems of laborious, time-consuming, long-term, etc., and achieve the effect of significant anti-cancer activity

Active Publication Date: 2022-07-05
湖南省中医药研究院
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  • Abstract
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  • Application Information

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Problems solved by technology

Although the traditional extraction, separation and analysis methods have formed a very complete system, there are still shortcomings such as time-consuming, laborious, and long cycle, which cannot meet and adapt to modern Chinese medicine research.

Method used

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  • A kind of Tianmingjing extract and preparation method and application in anti-hepatocellular carcinoma active drug through jak2/stat3 channel
  • A kind of Tianmingjing extract and preparation method and application in anti-hepatocellular carcinoma active drug through jak2/stat3 channel
  • A kind of Tianmingjing extract and preparation method and application in anti-hepatocellular carcinoma active drug through jak2/stat3 channel

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Embodiment 1

[0029] 1. The method used in this embodiment:

[0030] 1. Preparation of Tianming Jing Extract

[0031] Take Tianmingjing whole herb (2Kg), dry and pulverize it, soak it in 95% ethanol with a volume percentage concentration of 10 times the mass of Tianmingjing whole herb for 24h, extract three times, mix the three extracts, and dry to obtain the total extract ( TEC) 458g. Dissolve TEC (400g) with 2% NaOH solution by mass until pH=9, then adjust pH to 2 with 1% concentrated hydrochloric acid to obtain precipitation, and finally obtain total extraction of total sesquiterpene lactones Substance (SLEC) (42g).

[0032] 2. Screening of antitumor activity by cck-8 method

[0033] Take the cells in the logarithmic growth phase, digest and count, with 3*10 3 Cells / well density was seeded in a 96-well plate, 100 μL per well. Three replicate wells were set in each group. After culturing adherent, the TEC site, SLEC site and residual samples with different concentrations (50, 100, 15...

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Abstract

The invention belongs to the technical field of traditional Chinese medicine, and discloses a Tianming Jing extract, a preparation method and an application in an active drug against liver cancer through JAK2 / STAT3 channel. Including the following operation steps: take the whole plant of Tianmingjing, dry and pulverize, soak and extract with ethanol with a concentration of 95% by volume for 24 hours, extract three times, and obtain the total extract after the extract is mixed and dried; NaOH solution is added to the total extract to dissolve until pH= 9. Stop adding the NaOH solution, then adjust the pH to 2 with concentrated hydrochloric acid to obtain precipitation to obtain the total extract of Tianmingjing; separate the total extract of Tianmingjing by high-speed countercurrent chromatography to obtain three compounds, which are respectively the structural formula C 15 H 22 O 4 The compound 1 of formula C 15 H 20 O 3 The compound 2 of formula C 15 H 20 O 3 compound 3.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a Tianmingjing extract, a preparation method and an application in an active drug against liver cancer through JAK2 / STAT3 channel. Background technique [0002] Tianmingjing is a genus of Compositae, with about 21 species, mainly distributed in southern Europe and Asia. Among them, there are 17 species in the mountainous areas of southwest China. As a famous ethnic medicine, Tianmingjing has a long history of medicinal use in ethnic minorities such as Tujia, Bai, and Miao. Studies have found that it mainly contains chemical components such as sesquiterpene lactones, and most of them contain α-methylene-γ-butyrolactone, which has anti-cancer, anti-inflammatory, antibacterial and other biological activities. In the early stage, it was found that the total sesquiterpene lactone extract (SLEC) had a good effect on HepG-2 cells by screening the activ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D307/93A61K36/28A61P35/00
CPCA61K36/28A61P35/00A61K2236/333A61K2236/39A61K2236/53A61K2236/51A61K2236/55A61K2236/15
Inventor 沈冰冰张水寒杨玉佩成飞蔡媛刘雪辉
Owner 湖南省中医药研究院
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