A kind of Tianmingjing extract and preparation method and application in anti-hepatocellular carcinoma active drug through jak2/stat3 channel
An active drug, anti-cancer technology, applied in anti-tumor drugs, drug combinations, plant/algae/fungus/moss components, etc., can solve the problems of laborious, time-consuming, long-term, etc., and achieve the effect of significant anti-cancer activity
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[0029] 1. The method used in this embodiment:
[0030] 1. Preparation of Tianming Jing Extract
[0031] Take Tianmingjing whole herb (2Kg), dry and pulverize it, soak it in 95% ethanol with a volume percentage concentration of 10 times the mass of Tianmingjing whole herb for 24h, extract three times, mix the three extracts, and dry to obtain the total extract ( TEC) 458g. Dissolve TEC (400g) with 2% NaOH solution by mass until pH=9, then adjust pH to 2 with 1% concentrated hydrochloric acid to obtain precipitation, and finally obtain total extraction of total sesquiterpene lactones Substance (SLEC) (42g).
[0032] 2. Screening of antitumor activity by cck-8 method
[0033] Take the cells in the logarithmic growth phase, digest and count, with 3*10 3 Cells / well density was seeded in a 96-well plate, 100 μL per well. Three replicate wells were set in each group. After culturing adherent, the TEC site, SLEC site and residual samples with different concentrations (50, 100, 15...
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