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Total alkaloids of Ailanthus sinensis root bark and its preparation method and application

A technology of total alkaloids and twig root bark, applied in the field of preparation of total alkaloids with anti-liver cancer activity in root bark

Active Publication Date: 2021-07-09
SHENYANG PHARMA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Before the present invention, there is no report on the anti-liver cancer activity of the total alkaloids of the root bark of Ailanthus sinensis

Method used

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  • Total alkaloids of Ailanthus sinensis root bark and its preparation method and application
  • Total alkaloids of Ailanthus sinensis root bark and its preparation method and application

Examples

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Embodiment 1

[0021] Take 1 kg of dried Ailanthus root bark, crush it into coarse powder, extract 3 to 5 times with 75-85% methanol or ethanol, and concentrate under reduced pressure to obtain Ailanthus root bark extract; ~5% lactic acid solution was dissolved and filtered to obtain a 0.8g / ml solution; the obtained acid solution was dynamically adsorbed with cation exchange resin Amberlite IR-116, and 4~6 pieces of 4 retention volume were eluted with 4~6% ammonia water , with PH=2~3, concentration is 60~80% ethanol eluting 4~6 retention volumes, recovery solvent obtains the crude extract of total alkaloids of Ailanthus root bark; Dissolve with a formic acid solution with a pH of 2 to 3, filter to remove the residue, and prepare a solution of 0.8 to 1.2 g / ml, then use NaOH solution to adjust the pH to 10 to 12; use chloroform to extract the obtained lye, and recover the chloroform to obtain 4.16g total alkaloids of Ailanthus sinensis root bark. Utilizing ultraviolet-visible light spectropho...

Embodiment 2

[0023] Take 1 kg of dried Ailanthus root bark, crush it into coarse powder, extract 3 to 5 times with 75-85% methanol or ethanol, and concentrate under reduced pressure to obtain Ailanthus root bark extract; ~5% lactic acid solution was dissolved and filtered to obtain a 0.8g / ml solution; the obtained acid solution was dynamically adsorbed using cation exchange resin Dowex50×1, and 4~6 pieces of 4 retention volume were eluted with 4~6% ammonia water, Use PH=2~3, concentration is 60~80% ethanol eluting 4~6 retention volumes, reclaim solvent and obtain the crude extract of total alkaloids of Ailanthus root bark; Dissolve the formic acid solution with pH=2~3, filter, remove the residue, make a solution of 0.8~1.2g / ml, use NaOH solution to adjust pH=10~12; use chloroform to extract the obtained lye, recover the chloroform to obtain 4.54 gAilanthus root bark total alkaloids. Utilizing ultraviolet-visible light spectrophotometry, using canthin-6-one as a reference substance, the ab...

Embodiment 3

[0025] Take 1 kg of dried Ailanthus root bark, crush it into coarse powder, extract 3 to 5 times with 75-85% methanol or ethanol, and concentrate under reduced pressure to obtain Ailanthus root bark extract; ~5% lactic acid solution was dissolved and filtered to obtain a 0.8g / ml solution; the obtained acid solution was dynamically adsorbed with cation exchange resin Diaion SK-103, and 4~6 pieces of 4 retention volume were eluted with 4~6% ammonia water , with PH=2~3, concentration is 60~80% ethanol eluting 4~6 retention volumes, recovery solvent obtains the crude extract of total alkaloids of Ailanthus root bark; Dissolve with a formic acid solution with a pH of 2 to 3, filter to remove the residue, and prepare a solution of 0.8 to 1.2 g / ml, then use NaOH solution to adjust the pH to 10 to 12; use chloroform to extract the obtained lye, and recover the chloroform to obtain 3.92g total alkaloids of Ailanthus sinensis root bark. Utilizing ultraviolet-visible light spectrophotom...

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Abstract

The invention belongs to the technical field of medicine, and relates to total alkaloids of the root bark of Ailanthus ailanthus extracted from the root bark of the plant Ailanthus sinensis and a preparation method thereof. The crude extract is obtained by ion exchange resin. After dissolving the crude extract with formic acid, adjust the pH to 10-12 with NaOH, and finally extract with chloroform, recover the solvent under reduced pressure, and dry to obtain the total alkaloids of Ailanthus sinensis root bark with high content. The total alkaloids provided by the invention have good anti-liver cancer activity and can be developed into a new anti-tumor drug. The method has the advantages of reasonable design, simple process technology and industrialized production.

Description

technical field [0001] The invention relates to the technical field of plant medicine, and relates to total alkaloids in the root bark of Ailanthus ailanthii and its preparation method and application, in particular to a method for preparing the total alkaloids in the root bark of the medicinal plant Ailanthus ailanthus with anti-liver cancer activity. Background technique [0002] Liver cancer has become a serious health problem in our country. Therefore, finding effective therapeutic drugs for liver cancer has become an urgent problem to be solved. Ailanthus (Altissima) is a plant of the genus Ailanthus in the family Simaroubaceae. It is named Ailanthus ailanthus because of the bitter taste of its bark and leaves and the special odor emitted by the glands at the base of the leaflets. It is distributed all over my country except Heilongjiang, Jilin, Xinjiang, Qinghai, Ningxia, Gansu and Hainan. Toon root bark, also known as Toon white bark or Ailanthus bark, is the dried...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/185A61P1/16A61P35/00A61K125/00
CPCA61K36/185A61K2236/333A61K2236/39A61K2236/55A61P1/16A61P35/00
Inventor 宋少江黄肖霄段治康王雨曦鄢志扬
Owner SHENYANG PHARMA UNIVERSITY
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