A kind of alkaloid compound derived from Trichoderma auranthoderma and application thereof

A technology of alkaloids and Trichoderma citrinum, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as drugs that have not yet been seen, and achieve the effect of significant anti-tumor activity

Inactive Publication Date: 2016-05-04
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The study found that the alkaloid compound shown has anti-tumor activity, but there is no report on the chemical structure of the compound and the inhibitory activity of tumor cell proliferation, so there is no drug related to it in the market

Method used

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  • A kind of alkaloid compound derived from Trichoderma auranthoderma and application thereof
  • A kind of alkaloid compound derived from Trichoderma auranthoderma and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Fermentative production and separation and purification of the compound of embodiment 1

[0015] 1 Fermentation production

[0016] Fermentation culture of production bacteria: according to the conventional method of cultivating microorganisms, take Trichodermacitrinoviride. IBPT-4 (preserved in China Type Culture Collection Center on January 25, 2013, address: Wuhan University, Wuhan, China, The deposit number is: CCTCCNO: M2013055) appropriate amount, inoculated on the PDA solid slant medium and cultivated in an incubator at 28° C. for 4 days.

[0017] Take an appropriate amount of Trichodermacitrinoviride. IBPT-4 that has been cultured on a slant for 4 days, and inoculate it into 400 mL of culture fluid [Culture fluid composition (g / L): mannitol 20.0, yeast extract 3.0, maltose 20.0, monosodium glutamate 10.0, Glucose 10.0, KH 2 PO 4 0.5, MgSO 4 0.3, NaCl6.0 constant volume] in a 1000mL Erlenmeyer flask, after static culture at 28°C for 30 days, mycelium and ferm...

Embodiment 2

[0027] The test of embodiment 2 in vitro antitumor activity

[0028] 1 Experimental samples and experimental methods

[0029] Preparation of the tested sample solution The test sample is the pure product of the compound separated and purified in the above-mentioned implementation 1. Accurately weigh an appropriate amount of sample, and prepare a solution with the required concentration with methanol for activity measurement.

[0030] Cell lines and cell subculture The human melanoma A375 cell line was used, and the A375 cells were subcultured in RPMI-1640 medium containing 10% FBS at 37°C in an incubator filled with 5% carbon dioxide.

[0031] Cell Proliferation Inhibitory Activity Test Method

[0032] Human melanoma A375 cells in the logarithmic growth phase were obtained by tetrazolium salt (MTT) method, and the cell density was adjusted to 2×10 per ml. 5 Cells were inoculated into 96-well cell culture plates at 200 μl per well, at 37°C with 5% CO 2 for 4 hours in an inc...

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Abstract

The invention relates to an alkaloid compound from Trichoderma citrinoviride and an application thereof. The structure of the compound is characterized in that the compound contains a molecular skeleton of pentacyclic amide and contains an unsaturated ketone connecting a 8-carbon saturated aliphatic long-chain; a methyl group is connected at N; and there are two hydroxyl groups in the molecule. It is confirmed through experiments that the alkaloid compound has good antineoplastic activity and can be used to prepare a cell proliferation inhibitor or antineoplastic drug for antineoplastic researches.

Description

technical field [0001] The invention relates to an alkaloid compound derived from Trichoderma auranthoderma and application thereof. Background technique [0002] Alkaloids are a type of nitrogen-containing organic compounds produced by biological secondary metabolism. There are many types of alkaloids in nature, most of which come from plants, so they are also called plant alkaloids. Alkaloids have important physiological effects on humans and animals, including anti-asthma and antitussive, hypoglycemic, hypolipidemic, antibacterial, antitumor, analgesic, etc., among which antibacterial and antitumor activities are the most prominent. Natural structural alkaloids are an important source for discovering lead compounds in the research of innovative drugs, and there are nearly a hundred kinds of alkaloid drugs currently used in clinical practice. Studies have found that some marine fungi can produce alkaloids with novel structures and good activities in the process of seconda...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D207/44A61K31/4015A61P35/00C12P17/10C12R1/885
CPCC07D207/456C12P17/10
Inventor 陈立张维维宫美维张其清
Owner FUZHOU UNIV
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