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Alkaloid compound from Trichoderma citrinoviride and application thereof

A compound and application technology, applied in the field of alkaloid compounds, can solve problems such as drugs that have not yet been seen, and achieve significant anti-tumor activity

Inactive Publication Date: 2014-12-17
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The study found that the alkaloid compound shown has anti-tumor activity, but there is no report on the chemical structure of the compound and the inhibitory activity of tumor cell proliferation, so there is no drug related to it in the market

Method used

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  • Alkaloid compound from Trichoderma citrinoviride and application thereof
  • Alkaloid compound from Trichoderma citrinoviride and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Fermentative production and separation and purification of the compound of embodiment 1

[0015] 1 Fermentation production

[0016] Fermentation culture of production bacteria: according to the conventional method of cultivating microorganisms, take Trichoderma auranthoderma ( Trichoderma citrinoviride. ) IBPT-4 (preserved in China Type Culture Collection Center on January 25, 2013, address: Wuhan University, Wuhan, China, deposit number is: CCTCC NO: M 2013055) appropriate amount, inoculated on PDA solid slant medium Cultivate for 4 days in a 28°C incubator.

[0017] Get the Trichoderma aeruginosa ( Trichoderma citrinoviride. ) appropriate amount of IBPT-4, inoculated into 400mL culture medium [Culture medium composition (g / L): mannitol 20.0, yeast extract 3.0, maltose 20.0, monosodium glutamate 10.0, glucose 10.0, KH 2 PO 4 0.5 , MgSO 4 0.3, NaCL 6.0 constant volume] in a 1000mL Erlenmeyer flask, after 30 days of static culture at 28°C, the mycelia and fermenta...

Embodiment 2

[0027] Example 2 Test of anti-tumor activity in vitro

[0028] 1 Experimental samples and experimental methods

[0029] Preparation of the tested sample solution The test sample is the pure product of the compound separated and refined in the above-mentioned implementation 1. Accurately weigh an appropriate amount of sample, and prepare a solution with the required concentration with methanol for activity measurement.

[0030] Cell lines and cell subculture The human melanoma A375 cell line was used, and the A375 cells were subcultured in RPMI-1640 medium containing 10% FBS at 37°C in an incubator filled with 5% carbon dioxide.

[0031] Cell Proliferation Inhibitory Activity Test Method

[0032] Tetrazolium salt (MTT) method Take human melanoma A375 cells in the logarithmic growth phase, adjust the cell density to 2×10 per ml 5 Cells were inoculated into 96-well cell culture plates at 200 microliters per well, and filled with 5% CO at 37°C 2 for 4 hours in an incubator. A...

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Abstract

The invention relates to an alkaloid compound from Trichoderma citrinoviride and an application thereof. The structure of the compound is characterized in that the compound contains a molecular skeleton of pentacyclic amide and contains an unsaturated ketone connecting a 8-carbon saturated aliphatic long-chain; a methyl group is connected at N; and there are two hydroxyl groups in the molecule. It is confirmed through experiments that the alkaloid compound has good antineoplastic activity and can be used to prepare a cell proliferation inhibitor or antineoplastic drug for antineoplastic researches.

Description

technical field [0001] The invention relates to an alkaloid compound derived from Trichoderma auranthoderma and application thereof. Background technique [0002] Alkaloids are a type of nitrogen-containing organic compounds produced by biological secondary metabolism. There are many types of alkaloids in nature, most of which come from plants, so they are also called plant alkaloids. Alkaloids have important physiological effects on humans and animals, including anti-asthma and antitussive, hypoglycemic, hypolipidemic, antibacterial, antitumor, analgesic, etc., among which antibacterial and antitumor activities are the most prominent. Natural structural alkaloids are an important source for discovering lead compounds in the research of innovative drugs, and there are nearly a hundred kinds of alkaloid drugs currently used in clinical practice. Studies have found that some marine fungi can produce alkaloids with novel structures and good activities in the process of seconda...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61P35/00C12R1/885C12P17/10C07D207/44A61K31/4015
CPCC07D207/456C12P17/10
Inventor 陈立张维维宫美维张其清
Owner FUZHOU UNIV
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