Embryogenesis and plant strain regeneration method for hybrid somatic cell of slash pine and cuban pine

A Caribbean pine, embryogenesis technology, applied in plant regeneration, botanical equipment and methods, plant cells, etc., can solve the problems of asexual cutting propagation difficulties, limited seed sources for seed production, etc.

Inactive Publication Date: 2008-07-16
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
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  • Claims
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Problems solved by technology

[0024] The purpose of the present invention is to find an effective method for hybrid somatic embryogenesis and plant regeneration of slash pine and Caribbean pine hybrids, and provide industrialized A complete set of technology for rapid breeding of slash pine and Caribbean pine hybrid seedlings to solve the shortage of slash pine and Caribbean pine hybrid seedlings in the current industrial afforestation

Method used

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  • Embryogenesis and plant strain regeneration method for hybrid somatic cell of slash pine and cuban pine
  • Embryogenesis and plant strain regeneration method for hybrid somatic cell of slash pine and cuban pine
  • Embryogenesis and plant strain regeneration method for hybrid somatic cell of slash pine and cuban pine

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Experimental program
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Embodiment Construction

[0049] The immature embryos of hybrid pine were collected from a seed garden in Guangdong and repeated for two years. The cones have been collected since the beginning of June and collected once a week until the embryos of the cones have matured in early August. Wrap the collected cones with wet gauze to prevent the cones from dehydration, wrap them in plastic bags, and store them in a refrigerator at 4°C (at least one week, low temperature is conducive to the induction of embryogenic callus).

[0050] Remove the seed coat and inner and outer testa of immature seeds, first disinfect with 75% alcohol for 30 seconds, rinse with sterile water for 3-5 times; then disinfect with 1:10 84 disinfectant for 8 minutes, and rinse with sterile water for 3-5 times. Seeds with endosperm are directly inoculated into the medium.

[0051] Using the above three different modified P6 medium, add 1-2mg / L 2,4-D and 0.5-2mg / L BA, glutamine (Glu) 1000mg / L, hydrolyzed casein (CH) 500mg / L, muscle Al...

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Abstract

A method for somatic cell embryo generation and plant regeneration of a hybrid of a slash pine and a Caribbean pine is characterized in that the invention relates to a method which adopts a immature zygotic embryos of the hybrid of the slash pine and the Caribbean pine to carry out somatic cell embryo generation and plant regeneration and uses liquid and slide improved P6 mediums to alternatively combine for adjustment and control culture during the process of the somatic cell embryo generation and plant regeneration for a plurality of times and is appended with proper auxin combination. The invention provides a packaged technology for fast breeding the hybrid seedlings of the slash pine and the Caribbean pine industrializedly and can solve the current problem of short supply of the seedlings during the hybrid afforestation of the slash pin and the Caribbean pine.

Description

1. Technical field [0001] The invention belongs to the technical field of plant regeneration through tissue culture in forestry, and specifically relates to a method for hybrid somatic embryogenesis and plant regeneration of slash pine and caribbean pine. 2. Background technology [0002] Slash pine (Pinus elliottii Englem) is native to the southeastern United States, and Caribbean pine (Pinuscaribaea Morelet) is native to South American countries such as Cuba and Honduras. Cuban Caribbean Pine. Slash pine has a wide range of adaptation in China and is relatively cold-resistant. It can be planted in Nanyang, Xinyang, Henan, and parts of the Shandong Peninsula; while Caribbean pine is weak in cold resistance, and it is often damaged by freezing in areas north of 23 degrees north latitude. There are no reports of interspecific hybrids between the two species in the natural state. [0003] The two introduced tree species grow rapidly in my country, and the flowering periods o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00C12N5/04
CPCY02A40/22Y02P60/40
Inventor 施季森于敏边黎明席梦利赵奋成
Owner NANJING FORESTRY UNIV
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