Anti-aging or antioxidant composition containing plant stem cell line derived from cambium of panax ginseng

Inactive Publication Date: 2011-04-28
UNHWA CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0007]It is an object of the present invention to a natural material-derived composition which has minimized side

Problems solved by technology

However, UV light, excessive physical and chemical stimuli from the external environment, stresses, nutritional deficiency and the like deteriorate the normal function of the skin and accelerate skin aging phenomena such

Method used

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  • Anti-aging or antioxidant composition containing plant stem cell line derived from cambium of panax ginseng
  • Anti-aging or antioxidant composition containing plant stem cell line derived from cambium of panax ginseng
  • Anti-aging or antioxidant composition containing plant stem cell line derived from cambium of panax ginseng

Examples

Experimental program
Comparison scheme
Effect test

example 1

Preparation of Cell Line Derived From Cambium of Wild Ginseng

[0052]1-1: Preparation of Plant Material

[0053]“A” of FIG. 1(a) shows the typical feature of wild ginseng used in the present invention. In order to use the main root of purchased wild ginseng, the main root was purchased, and then washed with running water to remove earth or other contaminants from the surface thereof, and the surface of the main root was washed with a liquid detergent. Then, the main root was allowed to stand under running water. The washed root tissue was placed in a sterilized flask in a clean bench and disinfected with 70% ethanol for a time ranging from about 30 seconds to about 1 minute. Then, it was rinsed with sterile water and treated with a disinfectant solution containing 1-1.5% sodium hypochlorite (Junsei, Japan) for about 5-15 minutes. At this time, in order to allow the disinfectant solution to effectively penetrate into the tissue, several drops of TWEEN 20 (polyoxyethylenesorbitan monolaura...

example 2

Preparation of Extract of Wild Ginseng Cambium-Derived Cell Line

[0076]Active substances were extracted in the following manner from three cell lines prepared in Example 1:

[0077](1) Preparation of DMSO (dimethyl sulfoxide) Extract

[0078](i) 500 g of each of the cell line, from which the medium has been removed, and the freeze-dried cell line, was dissolved in 500 ml of DMSO with stirring at 50° C. for 6 hours.

[0079](ii) After completion of the dissolution, the cell solution was centrifuged at 3,000 g for 10 minutes, and the supernatant was collected, thus obtaining a DMSO-soluble substance.

[0080](iii) The obtained DMSO-soluble substance was concentrated using a rotary vacuum concentrator.

[0081](iv) The concentrated sample was dried using a freeze dryer, thereby obtaining a DMSO extract.

[0082](2) Preparation of Distilled Water Extract, Methanol Extract and Acetone Extract

[0083](i) 500 g of each of the cell line, from which the culture medium has been removed, and the freeze-dried cell ...

example 3

Examination of Cytotoxicity

[0096]In order to determine the concentration of sample to be used in tests, a cytotoxicity test was performed in the following manner.

[0097]NHF (normal human fibroblast) cells used in the test were isolated from the fetal foreskin and cultured. A medium used in the cell culture was prepared by adding 10% fetal bovine serum (FBS, Hyclone, Logan, Utah, USA), inactivated by heating at 56° C. for 30 minutes, 100 unit / ml penicillin, 100 μg / ml streptomycin and 300 μg / ml-glutamine, to DMEM medium (Invitrogen Gibco life tech. Vienna, Austria). The cells were cultured in the above medium in a 5% CO2 incubator at 37° C. at a relative humidity of 95%, and subcultured at 3 to 4-day intervals immediately before the cells would be fused with each other. The NHF cells were used in the experiments of this Example and Examples 4 and 5 below.

[0098]The NHF(p5) cells (normal human fibroblast cells) were dispensed in 96-well plates at a density of 5,000 cells / well, and after ...

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Abstract

The present invention relates to an anti-aging or antioxidant composition which contains, as an active ingredient, a plant stem cell line derived from the cambium of Panax ginseng, including wild ginseng and ginseng, or an extract thereof, a lysate thereof and a culture thereof. The composition has minimized side effects compared to existing anti-aging agents and antioxidants, and thus is safe for the skin. Also, the composition of the present invention shows an antioxidant effect of inhibiting reactive oxygen species caused by exposure to UV radiation that is the major cause of skin aging, and it can effectively reduce or inhibit aging-related factors. Thus, the composition is useful for the prevention and inhibition of aging.

Description

TECHNICAL FIELD[0001]The present invention relates to an anti-aging or antioxidant composition containing, as an active ingredient, any one or more of a cell line derived from the cambium of Panax ginseng, including wild ginseng and ginseng, an extract thereof, a lysate thereof and a culture thereof.BACKGROUND ART[0002]There are about 300,000 species of plants in the world today, and it is known that plants contain useful components exhibiting various physiological activities. The useful components are secondary metabolites that are produced secondarily during normal metabolic processes and accumulated at intracellular specific sites. These useful components are receiving increasing attention, because they serve to defend plants from microorganisms or external animals from biochemical and ecological viewpoint and are advantageous in terms of physiological activity. Secondary metabolites that are produced by plants include alkaloids, flavonoids, carotenoids, glycosides, terpenoids, e...

Claims

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Application Information

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IPC IPC(8): A61K36/25C12N5/04A61P17/00
CPCA61K8/97A61Q19/08A61Q19/004A61K2800/522A61K8/9789A61P17/00A61P17/18A61P19/08A61P39/06A61P43/00A61K36/258
Inventor JANG, MI OKLIM, MIN JUNGOH, IL SEOKLEE, DAE HEELEE, EUN KYONGJIN, YOUNG WOO
Owner UNHWA CORP
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