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Specific gene controlling growth of Arabidopsis vascular bundle and application thereof

A technology of genes and coding genes, applied in the direction of using vectors to introduce foreign genetic material, recombinant DNA technology, etc., can solve problems such as limited effects, and achieve the effect of broad application prospects

Inactive Publication Date: 2010-09-08
PEKING UNIV
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Problems solved by technology

But relying solely on conventional breed selection, the effect is limited

Method used

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  • Specific gene controlling growth of Arabidopsis vascular bundle and application thereof
  • Specific gene controlling growth of Arabidopsis vascular bundle and application thereof
  • Specific gene controlling growth of Arabidopsis vascular bundle and application thereof

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Embodiment Construction

[0026] The present invention will be described in further detail below by way of examples.

[0027] 1. Screening of hca2 mutants with high cambium activity

[0028] There is no endogenous tagging factor in Arabidopsis, and T-DNA integration becomes the main method of insertional mutagenesis. Thousands of mutants have been obtained in Arabidopsis through T-DNA tagging. After TAIL-PCR (Thermal Asymmetric InterlacedPCR, Yaoguang Liu et al, 1998, Plant Molecular Biology Reporter 16: 175-181, Thermal Asymmetric Interlaced Polymerase Chain Reaction) technology amplified and determined the sequence at both ends of the tag insertion site, using Blast2.0 software compares the determined sequence with the whole genome sequence of Arabidopsis thaliana, finds the mutation site, plants the mutants, and screens out the control Arabidopsis among a large number of mutants through phenotype analysis and screening of various environmental conditions. Genes responsible for cambium development ...

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Abstract

The present invention discloses a specific gene controlling growth of Arabidopsis vascular bundle and application thereof. The gene codes the protein of amino acid sequence shown in SEQ ID No.2 in sequence list and the genome gene is AT5g62940; the corresponding cNDA sequence is shown in the SEQ ID No.1 of the sequence list. As shown from research, the gene codes a transcription factor which is positioned in a core and has trans-activation activity, but the transcription factor which can regulate the formation of the Arabidopsis interfascicular cambium and the growth of vascular tissue is the transcription factor which is found as the first transcription factor for regulating the interfascicular cambium in Arabidopsis. In a transgenic gene plant, overexpression of the gene can thicken the stem of the plant, but inhibiting the expression of the gene in the plant can inhibit the occurrence of the interfascicular cambium. Therefore, the gene can be used for regulating the thickness of the stem, researching the woody plants with growth strength of secondary growth, and improving the quality. The gene has wide application prospect in regulating thickness growth and growth of the wood, flowers and plants.

Description

technical field [0001] The invention belongs to the field of plant genetic engineering, and specifically relates to a gene for controlling the development of vascular bundles and stem thickness of Arabidopsis thaliana and its application in controlling the thickness of plant stems. Background technique [0002] The thickness of the tree is due to the continuous development of the vascular cambium in the vascular bundle tissue in the plant's conduction and support organ stems, that is, whether the annual rings formed by the development of the vascular bundles are continuously increasing. Therefore, regulating the developmental characteristics of the vascular cambium in plant stems can control the thickness of trees and the lodging resistance of crops, regulate the growth characteristics of tree branches and stems of crops, and promote the growth of tree growth and shape and the thickness and thickness of crop stems. , Coordinating the transportation and distribution of photos...

Claims

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Application Information

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IPC IPC(8): C12N15/82
Inventor 瞿礼嘉郭勇秦跟基康定明顾红雅
Owner PEKING UNIV
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