Anticancer composition comprising plant stem cell line derived from taxus cambium or procambium

a technology of procambium and stem cells, which is applied in the direction of biocide, plant/algae/fungi/lichens, drug compositions, etc., can solve the problems that the anticancer effect of the i>taxus /i>cambium- or procambium-derived cell line itself has not yet been reported, and achieves the effect of preventing and treating cancer and reducing side effects

Inactive Publication Date: 2010-10-28
UNHWA CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0008]It is an object of the present invention to provide a natural material-derived composition, which has mi

Problems solved by technology

Meanwhile, enormous research expenses have been paid for studies on the mechanism of development and treatment of cancers, but cancers still remain as incurable diseases, and various cancer

Method used

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  • Anticancer composition comprising plant stem cell line derived from taxus cambium or procambium
  • Anticancer composition comprising plant stem cell line derived from taxus cambium or procambium
  • Anticancer composition comprising plant stem cell line derived from taxus cambium or procambium

Examples

Experimental program
Comparison scheme
Effect test

example 1

Preparation of Taxus Cambium- or Procambium-Derived Cell Line

1-1: Preparation of Plant Material

[0071]Each of the twig and stem of Taxus spp. was collected, and then immediately soaked in 100 mg / L of the antioxidant, L-ascorbic acid (DUCHEFA, The Netherlands). Then, they were transported and stored.

[0072]Then, the plant was pretreated with a mixed solution of 1% benomyl (Dongbu Hannong Chemical, Korea), 1% daconil (Dongbu Hannong Chemical, Korea), 1% sterptomycin sulphate (DUCHEFA, The Netherlands) and 0.1% cefotaxime sodium (DUCHEFA, The Netherlands) for 24 hours, and then washed with tap water for 30 minutes to remove phenolic compounds and the remaining chemicals. Then, the plant was surface-sterilized in 70% ethanol (DC Chemical, Korea) for 1 min, 30% hydrogen peroxide (LG Chemical, Korea) for 15 min, 1% CLOROX solution for 15 min and 3% CLOROX solution for 5 min, and then washed 3-4 times with water.

1-2: Isolation of Procambium and Cambium Tissues from Twig and Stem

[0073]The out...

example 2

Preparation of Extract of Taxus Procambium- or Cambium-Derived Cell Line

[0085]From the cell line prepared in Example 1, active ingredients were extracted stepwise as follows.[0086](i) 500 g of the cell line from which the medium has been removed was dissolved in 500 ml of distilled water at 50° C. for 6 hours while being stirred.[0087](ii) After completion of the dissolution, the cell solution was centrifuged at 3,000 g for 10 minutes, and the supernatant was collected, thus obtaining a distilled water-soluble substance.[0088](iii) After obtaining the distilled water-soluble substance, the remaining distilled water-insoluble substance was dissolved in 500 ml of methanol at room temperature for 6 hours while being stirred.[0089](iv) After completion of the dissolution, the solution was centrifuged at 3,000 g for 10 minutes, and the supernatant was collected, thus obtaining a methanol-soluble substance.[0090](v) After obtaining the methanol-soluble substance, the remaining methanol-in...

example 3

Measurement of Antioxidant Activity of Taxus Cambium- or Procambium-Derived Cell Line Extract

[0094]Because the correlation between an antioxidant effect and the prevention of cancer is known, the following test was carried out in order to examine whether the cell line according to the present invention has the effect of preventing cancer.

3-1: Culture of Human Diploid Fibroblasts (HDF)

[0095]HDF cells were isolated from the fetal penis prepuce and cultured. The culture medium was prepared by adding 10% fetal bovine serum (FBS, Hyclone, Logan, Utah, USA) inactivated by heating at 56° C. for 30 minutes, 100 unit / ml of penicillin, 100 μg / ml of streptomycin and 300 μg / ml of glutamine to DMEM medium (Invitroge Gibco life tech. Vienna, Austria). The cells were cultured in the medium, described above, in a 5% CO2 incubator at a temperature of 37° C. and a humidity of 95% and subcultured at 3-4-day intervals, immediately before the cells were fused with each other. The subcultured cells were ...

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Abstract

The present invention relates to a composition for preventing or treating cancer, which contains, as an active ingredient, a Taxus cambium- or procambium-derived cell line; a lysate thereof; an extract thereof; or a culture medium thereof. The cell line, the lysate, the extract, and the culture medium has minimized side effects compared to the conventional therapeutic drugs, is safe to the human body, is involved directly in the growth of cancer to induce cancer cell death, and shows anticancer activity of inhibiting angiogenesis occurring in carcinogenesis. Accordingly, the cell line, the lysate, the extract and the culture medium is useful for the prevention, treatment and alleviation of cancer.

Description

TECHNICAL FIELD[0001]The present invention relates to a composition for preventing or treating cancer, which contains, as an active ingredient, a Taxus cambium- or procambium-derived cell line; a lysate thereof; an extract thereof; or a culture medium thereof.BACKGROUND ART[0002]Apoptosis is an active process of cell death that occurs through the regulation of various gene expressions and protein activities in cells according to programmed signals. Apoptosomes formed through this process are removed by the action of phagocytes such as the surrounding cells or as macrophages, such that they do not cause inflammation. Such apoptosis is frequently observed in various normal physiological processes of living organisms and known to be deeply involved in the process of development of various diseases. Namely, the development of abnormal apoptosis can lead to neurodegenerative disorders, immune disorders and cardiovascular diseases, and the abnormal inhibition of apoptosis can cause cancer...

Claims

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Application Information

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IPC IPC(8): A61K35/00A61K36/13A61P35/00
CPCA61K36/13A61P17/18A61P35/00C12N5/04
Inventor PARK, JOONG HYUNLIM, MIN JUNGOH, IL SEOKLEE, DAE HEELEE, JUNG CHANGLEE, EUN KYONGJIN, YOUNG WOO
Owner UNHWA CORP
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