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Stem cell tissue culture seedling raising technique

A technology of stem cells, technology, applied in the fields of plant cells, plant regeneration, horticulture, etc.

Inactive Publication Date: 2015-09-23
广东融和生态农业集团有限公司
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  • Abstract
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  • Application Information

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Problems solved by technology

However, due to the complex and unique chemical structures of many natural products, there are also many biosynthetic pathways involved, such as paclitaxel (20-step reaction), camptothecin (11-step reaction), vincristine (18-step reaction) and other compounds. Biosynthesis involves a series of complex enzymatic reactions, and chemical synthesis requires dozens of steps

Method used

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Experimental program
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Embodiment Construction

[0013] According to the different target stem cells, the current relatively successful plant stem cell culture methods mainly include the following:

[0014] 1, woody plant vascular cambium meristem (taking Taxus as an example): take the new shoots of wild Taxus, cut after surface sterilization; gently remove the tissue containing cambium, phloem, cortex and epidermis Xylem stripping; after the obtained tissue is cultured on the separation medium for 30 days, the new cambium cells and other dedifferentiated cells (callus) are naturally separated due to the difference in the tissue status - the cambium cells are uniformly growing plate-like tissue , and callus is an irregular aggregate growth; the acquired cambium cells are transferred to growth medium for culture.

[0015] After completing the initial establishment of the culture system, the researchers first investigated the morphology, genetic characteristics and genomics of the obtained cells to verify that the cultured cel...

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Abstract

The invention discloses a stem cell tissue culture seedling raising technique. The technique comprises the steps of 1, taking a fresh plant, conducting sterilization on the surface and cutting the plant; 2, conducting tissue differentiation and reserving a cambium; 3, culturing the cambium on an isolation medium. According to the technique, existing methods are improved based on a traditional tissue culture technique, plant stem cells are taken as a target, induction, separation and explant culture are conducted, and a corresponding stem cell culture system is established. By the adoption of the plant stem cell culture technique, the plant culture technique is enriched, and a novel research direction and opportunity is provided for commercialized production of natural products and development of the plant biotechnology.

Description

technical field [0001] The invention relates to a stem cell group culture seedling technology. Background technique [0002] As the main source of lead compounds and drugs, natural products of plant origin have played a very important role in the development of the pharmaceutical industry. In recent years, its status has gradually declined, mainly due to the slow growth of natural plants and low content of active ingredients, which makes it difficult to guarantee the source of such compounds and meet the needs of industrialized mass production in the modern pharmaceutical industry. [0003] In view of the important medicinal potential and economic value of natural compounds, with the development of modern science, scholars in various fields have given their own answers to their sources. The more effective research mainly focuses on the following aspects: (1) Express the synthetic pathway of natural products in microorganisms, and use genetically engineered bacteria to ferme...

Claims

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Application Information

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IPC IPC(8): A01H4/00C12N5/04
Inventor 欧小鲁古巧云钟珊
Owner 广东融和生态农业集团有限公司
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