Apple stem cell culture method and apple stem cells cultured by method

An apple stem cell and cell technology, applied in plant cells, botany equipment and methods, and plant preservation, can solve the problems of prolonging the preparation cycle of stem cells, complicated preparation methods, and reduced concentration of active substances

Inactive Publication Date: 2015-06-17
GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the addition of liposomes will undoubtedly lead to the extraction of the reduced concentration of active substances, and the preparation method of liposomes is relatively complicated, which prolongs the preparation cycle of stem cells again.
Moreover, liposomes are mostly very sensitive to the environment in the gastrointestinal tract, and their oral administration can easily lead to the degradation of active substances or the reduction of bioavailability

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0138] The sterilization of embodiment 1 apple branch

[0139] Choose green-planted apple trees free of chemical fertilizer and pesticide pollution, and select 10 10cm-long new shoots according to certain criteria; wash the surface attachments on the new shoots with 2L of sterile water; soak the new shoots in 120mg / L L-ascorbic acid 1 minute; sterilize new shoots in 75% ethanol solution for 1 minute, rinse with 2L sterile water; put them in 30% hydrogen peroxide solution for sterilization for 20 minutes, and rinse with sterile water for 10 minutes.

Embodiment 2

[0140] Example 2 Induction of cambium tissue

[0141] Peel off tissues such as cambium, phloem, cortex and epidermis with an inoculation knife from the sterilized apple shoots of Example 1, discard xylem and pith; inoculate the obtained tissue into fresh solid medium, and inoculate 10 cm per 1 g of tissue 2 The density of the solid medium is inoculated and cultured in a controlled dark room. After culture, the cambium tissue is a flat plate-shaped tissue with uniform growth, while other tissues are irregular aggregate growths. The obtained cambium tissue is weighed .

[0142] Table 1 culture conditions

[0143]

Embodiment 3

[0144] Embodiment 3 Subculture Cambial Tissue

[0145] Separate the regenerated cambium tissue from other tissues; inoculate 10 cm per 1 g of tissue 2 The density of the solid medium, the regenerated cambium tissue was inoculated into a fresh solid medium, cultured in a dark room, and the callus was separated and weighed.

[0146] Table 2 culture conditions

[0147]

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PUM

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Abstract

The invention relates to the technical field of plant stem cell culture, particularly an apple stem cell culture method and apple stem cells cultured by the method. The method comprises the following steps: by using an apple new branch as a raw material, inducing to form callus, and carrying out enrichment culture and amplification culture to obtain the apple stem cells. The method provided by the invention has the advantages of shorter period and high yield. The method can obtain abundant apple stem cells within about 48 days. The induction culture medium used in the apple stem cell culture process and the variety of the phytohormones contained in the enrichment culture medium are proper and suitable in proportion, and can ensure fast dedifferentiation and abundant reproduction of apple branch cells. The apple stem cells can be freeze-dried or extracted and used for preparing food, drugs, health products or cosmetics. The apple stem cell extraction method provided by the invention is simple and easy to implement and has higher extraction efficiency. The prepared extract contains abundant flavones and polyphenols.

Description

technical field [0001] The invention relates to the technical field of plant stem cell culture, in particular to a method for culturing apple stem cells and cultivated apple stem cells. Background technique [0002] Plant stem cells (plant stem cells) are undifferentiated cells with strong self-renewal and continuous division capabilities. Plant stem cell culture technology is based on traditional tissue culture technology, aiming at plant stem cells, inducing, isolating and cultivating explants, and establishing a corresponding stem cell culture system. Plant stem cell culture technology not only enriches plant culture technology, but also provides The commercial production of natural products and even the development of plant biotechnology provide new research directions and opportunities. [0003] According to the different target stem cells, the current relatively successful plant stem cell culture methods are mainly divided into three types: vascular cambium meristem o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/04A01N3/00A23L1/29A23L33/00
Inventor 陈海佳王一飞葛啸虎戴国胜程建强
Owner GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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