Composition for Preventing or Treating AIDS Containing Plant Stem Cell Line Derived from Cambium of Panax Ginseng Including Wild Ginseng or Ginseng as Active Ingredient

a technology of acquired immunodeficiency syndrome and plant stem cells, applied in the field of compound for preventing or treating acquired immunodeficiency syndrome (aids), can solve the problems of inability to exhibit complete therapeutic effects without severe adverse side effects, failure to meet the requirements of clinical trials, etc., to achieve the effect of reducing adverse side effects

Inactive Publication Date: 2011-09-08
UNHWA CORP
View PDF5 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0008]It is an object of the present invention to provide a natural product-derived compound for the prevention and treatment of acquired immunodeficiency syndrome (AIDS) having a minimized adverse side effects compared to prior therapeutic agents.

Problems solved by technology

The cell entry of HIV depends on gp120 which binds to intracellular CD4 receptor molecules, and HIV infection causes depletion of CD4+ T cells, thereby leading to immunological incompetence, opportunistic infection, dysneuria, neoplasia, and ultimately death.
Until now, several anti-HIV agents, including 2,3-azidothymidine (AZT), dideoxyinosine (ddI), dideoxycytidine (ddC), and others have been approved by FDA in the United States, but they failed to exhibit complete therapeutic effects without severe adverse side effects.
Thus, the development of novel medicaments has become the critical issue.
However, the agents have side effects such as diabetes, hemolysis, hemorrhage, hyperlipidemia, impaired lipid metabolism, and others, and causes serious problem of the emergence of tolerant cell lines due to the long-term administration.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Composition for Preventing or Treating AIDS Containing Plant Stem Cell Line Derived from Cambium of Panax Ginseng Including Wild Ginseng or Ginseng as Active Ingredient
  • Composition for Preventing or Treating AIDS Containing Plant Stem Cell Line Derived from Cambium of Panax Ginseng Including Wild Ginseng or Ginseng as Active Ingredient
  • Composition for Preventing or Treating AIDS Containing Plant Stem Cell Line Derived from Cambium of Panax Ginseng Including Wild Ginseng or Ginseng as Active Ingredient

Examples

Experimental program
Comparison scheme
Effect test

example 1

Preparation of Homogeneous Cell Line Derived from Cambium of Panax Ginseng

[0049]1-1: Preparation of Plant Materials

[0050](1) FIG. 1(a)-A shows typical appearance of wild ginseng used in the present invention. Wild ginseng was purchased, washed with running water to remove soil, dirt, or other contaminants on its surface, its main root was again washed with a liquid detergent, and kept in running water. The washed root was placed into a sterilized flask in a clean bench, and then sterilized with 70% ethanol for about 30 sec to 1 min. Then, the tissue was rinsed out with sterile distilled water, and treated with an antiseptic solution containing 1% to 1.5% of sodium hypochlorite (Junsei, Japan) for 5 to 15 min. At this time, several drops of TWEEN® 20 (Polyoxyethylenesorbitan monolaurate, Junsei, Japan) were added to allow the antiseptic solution to effectively penetrate into the tissue. After the process, the main root was rinsed out with sterilized water 3 to 5 times. Then, in orde...

example 2

Drying of Homogeneous Cell Line Derived from Wild Ginseng Cambium and Preparation of Extract Thereof

[0075]The homogeneous cell line derived from the cambium of wild ginseng adventitious root of Example 1 was dried and extracted as follows:

[0076](1) Preparing Dried Cell Line;

[0077](i) The cell line isolated from culture medium was freeze dried or hot-air dried;

[0078](ii) The cell line was pulverized using a pulverizer.

[0079](2) Preparation of Extract Using Distilled Water

[0080](i) 500 g of the cell lines, which were isolated from culture medium, were hot-air dried, or were freeze dried, were extracted with 5000 ml of distilled water while shaking for 6 hour at 50° C.;

[0081](ii) After said dissolution, the extract was centrifuged at 3,000 g for 10 min and the supernatant was collected to obtain a distilled water-soluble product; and

[0082](iii) The distilled water-soluble product was concentrated under a reduced pressure using a rotary vacuum concentrator.

[0083](3) Preparation of Extra...

experimental example 1

Verification on Preventive and Therapeutic Effects of Homogeneous Cell Line Derived from Cambium of Panax Ginseng on Acquired Immunodeficiency Syndrome (i)—Measurement of Cell Count Ratio of CD4+ T / CD8+ T

[0087]In order to verify the preventive and therapeutic effects of homogenous cell line derived from the cambium of Panax ginseng according to the present invention, the dried cell line powder prepared from Example 2(1) was administered to an AIDS patient (born in 1976, male, diagnosed with AIDS in August, 2006) with a positive HIV response. One gram of the powder was orally administered as a dissolved form in water for 9 days (date of initial administration: 19, August, 2008) three times a day. The patient was required not to take any other therapeutic agent for AIDS, and there was no adverse side effect shown.

[0088]After 9 days of administration, blood sample was taken from the patient, and then incubated with Simultest reagent (Becton dichknson, San Jose, Calif.) using phycoeryth...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The present invention relates to a composition for preventing or treating acquired immunodeficiency syndrome (AIDS), comprising one or more of the following: a homogenous cell line, and a lysate, an extract and a culture thereof as an active ingredient. The homogenous cell line, the lysate, the extract and the culture thereof, which are derived from a natural product, minimize adverse side effects of prior therapeutic agents and safe for the human body. Further, they effectively increase the count of T cells such as CD4+ T cells and decrease the number of HIVs, thereby preventing opportunistic infection, dysneuria, and neoplasia caused by immune incompetence, and ultimately the risk of death. Therefore, they are useful in preventing and treating AIDS, and relieving symptoms of AIDS.

Description

FIELD OF THE INVENTION[0001]The present invention relates to a composition for preventing or treating acquired immunodeficiency syndrome (AIDS), comprising one or more of the following: a cell line derived from the cambium of Panax ginseng including wild ginseng and ginseng, and a lysate, an extract and a culture thereof, as an active ingredient.BACKGROUND OF THE INVENTION[0002]Since an AIDS (acquired immunodeficiency syndrome) patient was first found in 1982, in Los Angeles, USA, the number of patients has increased worldwide. In Korea, AIDS patients have steadily increased from the discovery of an American AIDS patient in 1985, requiring the development of new therapeutic agents.[0003]Human immunodeficiency virus (HIV) is an enveloped virus that attacks mainly human lymphocytes, and belongs to a member of the retrovirus family as it converts its viral RNA genome to DNA by reverse transcription and integrates the resulting DNA into the cellular DNA of a host cell. HIV has RNA as it...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(United States)
IPC IPC(8): A61K36/00A61K36/258A61P31/12
CPCA01H4/005A23L1/30A23L1/3002A23V2002/00A61K36/258C12N5/04A23V2200/30A23V2250/2124A23L33/10A23L33/105A61P31/12A61P31/18A61P37/00A61P37/04
Inventor HONG, SUN MILEE, EUN KYONGJIN, YOUNG WOO
Owner UNHWA CORP
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap