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Culture method of plant stem cells

A plant stem cell and culture method technology, applied in the field of plant stem cell culture, can solve the problems of cumbersome steps, regular culture cycle, unfavorable production, research, etc., and achieve the effect of high extraction efficiency and short culture cycle

Inactive Publication Date: 2018-03-27
HUAIBEI ZHIHUAI TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional corn stem cell culture method has a long culture cycle and cumbersome steps, which is not conducive to its production and research.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] A plant stem cell culture method, the specific steps of the plant stem cell culture method are as follows:

[0018] S1. Raw material selection, select corn with full and complete grains, bright and shiny color, and no mildew and corruption, first rinse it with clean water 1-3 times to remove impurities and dust, then use disinfectant to disinfect it, and use it after disinfection The dryer dries it until the moisture is completely removed;

[0019] S2, seed cultivation, the treated seeds are planted in the medium, and the temperature is 22 ° C, and the humidity is 95% and cultivated for 5 days to germinate;

[0020] S3, seed treatment, after the seed germinates for 4-5 days, the root tissue containing the quiescent center is collected, the root cap at the root end is removed, and the length of 1 mm is cut from the incision as an explant;

[0021] S4. Induction separation, the explants are placed in the induction medium, and after being cultured for 25 days under steril...

Embodiment 2

[0027] A plant stem cell culture method, the specific steps of the plant stem cell culture method are as follows:

[0028] S1. Raw material selection, select corn with full and complete grains, bright and shiny color, and no mildew and corruption, first rinse it with clean water 1-3 times to remove impurities and dust, then use disinfectant to disinfect it, and use it after disinfection The dryer dries it until the moisture is completely removed;

[0029] S2, seed cultivation, the treated seeds are planted in the medium, and the temperature is 23 ° C, and the humidity is 96% and cultivated for 4 days to germinate;

[0030] S3, seed treatment, after 4-5 days after seed germinates, the root tissue that will contain quiescent center is collected, removes the root cap of root end, begins to intercept 1.1mm long as explant from incision;

[0031] S4. Induction separation, the explants are placed in the induction medium, and after being cultured for 25 days under sterile, non-toxic...

Embodiment 3

[0037] A plant stem cell culture method, the specific steps of the plant stem cell culture method are as follows:

[0038] S1. Raw material selection, select corn with full and complete grains, bright and shiny color, and no mildew and corruption, first rinse it with clean water 1-3 times to remove impurities and dust, then use disinfectant to disinfect it, and use it after disinfection The dryer dries it until the moisture is completely removed;

[0039] S2, seed cultivation, the treated seeds are planted in the medium, and the temperature is 25 ° C, and the humidity is 98% and cultivated for 3 days to germinate;

[0040] S3, seed treatment, after 4-5 days after seed germination, the root tissue that will contain quiescent center is collected, removes the root cap of root end, begins to intercept 1.2mm long as explant from incision;

[0041] S4. Induction and separation, the explants are placed in the induction medium, and after being cultured for 25 days under sterile, non-...

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PUM

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Abstract

The invention discloses a culture method of plant stem cells. The culture method of plant stem cells specifically comprises the following steps: S1, raw material selection; S2, seed cultivation; S3, seed treatment; S4, inductive separation; and S5, cultural proliferation. According to the culture method of plant stem cells disclosed by the invention, the method comprises the following steps: by taking fresh corns as a raw material, culturing the raw material to obtain germinated seeds; then selecting root tissues with static centers; and inducing to obtain static central stem cells. Compared with a conventional plant stem cell culture method, the culture method plant stem cells disclosed by the invention is simple and feasible, high in extraction efficiency and short in cultural period.

Description

technical field [0001] The invention belongs to the field of cell culture, and more specifically relates to a plant stem cell culture method. Background technique [0002] Plant stem cells, also known as meristems, are relative to mature tissues that have differentiated and matured in plants. Plant meristems can be divided into apical meristems and lateral meristems according to their location. The apical meristem includes shoot apical meristem (SAM) and root apical meristem (RAM). Lateral meristem includes vascular cambium and cork cambium. The cultivation of plant cells is roughly divided into four types: 1. Tissue culture: callus is induced, and if the conditions are suitable, regenerated plants can be cultivated. It is used to study the growth and development, differentiation and genetic variation of plants; to carry out asexual reproduction; to obtain metabolites. 2. Suspension cell culture: a culture technology developed on the basis of callus culture technology. ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/005
Inventor 闫毛钦杨慧侠
Owner HUAIBEI ZHIHUAI TECH CO LTD
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