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Composition for enhancing immunity containing plant stem cell line derived from cambium of panax ginseng including wild ginseng or ginseng as active ingredient

A technology to enhance immunity and cell lines, applied to medical preparations containing active ingredients, plant cells, plant/algae/fungus/moss components, etc.

Active Publication Date: 2012-01-18
SHENZHEN LUQUAN IND CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

An extract for enhancing immunity derived from Korean seaweed was developed for enhancing immunity (Korean Laid-Open Patent No. 10-2000-00063617), but there has never been a demand for an excellent immune enhancer derived from natural products interrupted

Method used

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  • Composition for enhancing immunity containing plant stem cell line derived from cambium of panax ginseng including wild ginseng or ginseng as active ingredient
  • Composition for enhancing immunity containing plant stem cell line derived from cambium of panax ginseng including wild ginseng or ginseng as active ingredient
  • Composition for enhancing immunity containing plant stem cell line derived from cambium of panax ginseng including wild ginseng or ginseng as active ingredient

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Example 1. Preparation of homogenous cell lines derived from ginseng cambium

[0057] 1-1: Prepare plant material

[0058] figure 1 A of (a) shows the typical appearance of wild ginseng used in the present invention. After preparing wild ginseng (produced in Gangwon Province), in order to use the main root of wild ginseng, remove soil or other pollutants attached to the outer surface with running water, wash the surface of the main root with liquid detergent, and then put it in running water. Put the cleaned tissue into a sterilized flask prepared on a clean bench, and sterilize it with 70% ethanol for about 30 seconds to 1 minute. Rinse with sterile water afterwards, and carry out disinfection treatment with 1% to 1.5% sodium hypochlorite (sodium hypochlorite, Junsei, Japan) disinfectant solution for about 5-15 minutes. At this time, in order to allow the disinfectant to penetrate into the tissue effectively, a few drops of TWEEN 20 (polyoxyethylene sorbitan monolau...

Embodiment 2

[0106] Example 2. Drying of homogenous cell lines derived from wild ginseng cambium and preparation of its extracts

[0107] The cell lines prepared by the two treatment methods in Example 1 were dried and extracted according to the following methods.

[0108] (1) Preparation of crushed cell line dried by hot air

[0109] (i) The cell line from which the culture medium was removed was dried with hot air at 90°C.

[0110] (ii) The above-mentioned dried cell line was obtained by pulverizing with a pulverizer.

[0111] (2) Preparation of freeze-dried cell line pulverization

[0112] (i) Freeze the cell line from which the culture medium has been removed at ultra-low temperature (-70°C), then slowly depressurize and maintain it above normal temperature (25°C) for drying.

[0113] (ii) The above-mentioned dried cell line was obtained by pulverizing with a pulverizer.

[0114](3) Preparation of distilled water extract

[0115] (i) Take 500 g of the cell line removed from the cu...

Embodiment 3

[0125] Example 3. Cytotoxicity test

[0126] In order to determine the use concentration range of the samples used in the experiment, the following cytotoxicity test was carried out.

[0127] 3-1 Determination of the effect of sample administration on body weight change

[0128] First, the body weights of the control group and the experimental group were measured before sample administration, and the body weights before and after sample administration were compared. The control group was given only solid feed, while in experimental groups 1 and 2, the hot air-dried cell line pulverized material obtained in Example 2 above was mixed into the pulverized feed at 5% by weight, and the mice were allowed to eat freely. That is, the administration in the experimental group 1 was the cell line obtained in the above-mentioned Examples 1-6 after 14 days of suspension culture, and then the dry cell line pulverized product dried by hot air; the administration in the experimental group 2 ...

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Abstract

The present invention relates to a composition for enhancing immunity containing a cell line derived from the cambium of Panax ginseng including wild ginseng or ginseng, or the grounded substance, extract, or culture medium of the cell line as an active ingredient. The cell line and ground substance, extract, and culture medium of the cell line according to the present invention compose a natural product-derived composition. The cell line, ground substance, extract, and culture medium are safe to a human body by minimizing side effects of a conventional immunity booster, and also effectively increase activity of NK cells charging innate immunity. As well, the cell line, ground substance, extract, and culture medium appear to enhance acquired immunity by increasing proliferation rate of lymph node cells when re-exposing the cells to an antigen in a specific immune reaction, thus are useful as an immunity booster. Especially, the cell line, ground substance, extract, and culture medium also effectively increase the number of bone marrow cells, thus are not only used as an immune reaction adjuvant, but also used in preventing and treating anemia caused by hematopoiesis.

Description

technical field [0001] The present invention relates to a composition for enhancing immunity, which contains a ginseng-derived cambium cell line, its lysate, its extract or its culture solution as an active ingredient. Background technique [0002] Immunity can be roughly divided into two categories: innate immunity that is present at birth and acquired immunity that is acquired through adaptation to acquired life. Here, innate immunity is also called "natural immunity", which reacts non-specifically to antigens and does not have specific immune memory. The innate immune system includes skin, mucus tissue, and low-pH gastric acid that block antigen invasion, and phagocytes such as macrophages and polymorphonuclear leukocytes that undertake phagocytosis to remove invaders who invade the body. This innate immunity actually prevents most infections. In addition, since acquired immunity has a memory function for the first invasion antigen, it has the characteristics of carryin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/258A61P37/00
CPCA61K36/258A23V2002/00C12N5/04A01H4/005A23L1/3002A23L33/105A61P37/00A61P37/04A61P7/06A23V2200/324A23V2250/2124
Inventor 李银庆陈英雨林敏贞
Owner SHENZHEN LUQUAN IND CO LTD
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