Plant stem cell line derived from quiescent center and method for isolating the same

A cell line, plant technology, applied in plant cells, biochemical equipment and methods, microorganisms, etc., can solve problems such as high production cost, complex chemical structure, and little research

Inactive Publication Date: 2011-01-05
UNHWA CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

First, the content of physiologically active substances in plants is very limited
Second, plants grow very slowly
Fourth, lead to environmental problems related to natural destruction
Fifth, physiologically active substances derived from plants have very complex chemical structures, thus requiring multi-step polymerization processes, thereby causing economic problems of high production costs
However, in the case of animals, methods for isolating and culturing stem cells have long been established, whereas in the case of plants, little or no research has been done on the isolation of stem cells

Method used

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  • Plant stem cell line derived from quiescent center and method for isolating the same
  • Plant stem cell line derived from quiescent center and method for isolating the same
  • Plant stem cell line derived from quiescent center and method for isolating the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Example 1: Isolation of Cell Lines from the Quiescent Center of Rice Plants

[0063] 1-1: Preparation of plant material

[0064] The rice seeds were dehulled, surface sterilized with 70% alcohol for 1 minute, soaked in 2% sodium hypochlorite solution for 1 hour, and then washed once or twice with sterile water. The washed seeds were thoroughly washed with sterile water for 30 minutes, and then dried to completely remove moisture.

[0065] The dried seeds were inoculated into N6 medium (CHU MEDIUM, Chu C.C., Proc. Symp. Plant Tissue Cult., Peking, 43, 1978) and cultured at 25°C for 5 days to germinate them. The components of the N6 medium are shown in Table 1 below.

[0066] Table 1

[0067]

[0068] Then, root tissues containing quiescent centers were collected from germinated seeds cultured for 5-6 days. The root cap was removed from the root tip, and a 1 mm thick portion of the cut surface was collected as an explant.

[0069] 1-2: Induction and isolation of q...

Embodiment 2

[0077] Example 2: Isolation of cell lines from the quiescent center of maize plants

[0078] Corn seeds were germinated in the same manner as in Example 1-1, and explants containing quiescent centers were collected from the roots of germinated plants. Then, the collected explants were cultured in the same manner as in Example 1-2 in a medium containing 2,4-D, CPA, IAA, IBA, NAA, and picloram, respectively, and cells from the quiescent center were observed. Whether the system is induced.

[0079] results, such as Figure 4 As shown, when explants of maize plants containing quiescent centers were cultured, the cell lines were induced in the medium containing 2,4-D at the same rate as root explants of rice plants containing quiescent centers were cultured. way to observe. However, in the case of auxins other than 2,4-D, the cells were not induced after inoculation of the explants, and adventitious roots formed in the explants. exist Figure 4 Among them, "A" is the tissue cu...

Embodiment 3

[0081] Example 3: Characterization of Cell Lines from the Quiescent Center of Rice Plants

[0082] 3-1: Observation of morphological changes during long-term culture

[0083] The cell line from the quiescent center isolated in Example 1 was inoculated into a medium having the same composition as the cell line induction medium of Example 1-2, i.e. N6 medium containing 2 mg / L 2,4-D and allow it to proliferate. Proliferating quiescent center cells were observed after 4 and 16 weeks of cell growth. Meanwhile, as a control group, cell lines derived from other than the quiescent center were allowed to proliferate in the same manner as described above, and then the morphological changes of the cells were observed.

[0084] results, such as Figure 5 As shown, after 4 weeks of culture ( Figure 5 A) and 16 weeks ( Figure 5 Photographs other than A) Morphological changes were then observed in cell lines derived from root tissue. After 16 weeks of culture, some local differentiat...

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Abstract

The present invention relates to a cell line derived from the quiescent center of a plant and a method for isolating the same, more specifically, relates to a quiescent center-derived homogeneous cell line of single-cell origin, which is obtained from the quiescent center of a plant without needing a separate de-differentiation process, and a method for isolating the same.

Description

technical field [0001] The present invention relates to plant quiescent center-derived cell lines and methods for isolating the same, in particular to homogeneous cell lines derived from single cells obtained from the quiescent centers of plants without separate removal. Differentiation step. Background technique [0002] Plants have been used as food sources in the past, but their meaning as a food source has now expanded to include sources for a wide range of chemicals, including pharmaceuticals, perfumes, pigments, agricultural chemicals and dyes. In particular, since most useful substances derived from plants have physiological activities, including antiviral, antibacterial, anticancer, and antioxidant activities, plants are considered to be ideal sources from which new drugs can be developed, and active research is being conducted to elucidate many plant sources The relationship between the chemical structure of a substance and its activity. [0003] However, it is di...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/04
CPCC12N5/04C12N5/00
Inventor 俞英美李恩景洪顺美陈荣雨
Owner UNHWA CORP
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