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Method for efficiently acquiring gerbera jamesonii detoxification seedling

A seedling and high-efficiency technology, which is applied in the field of efficient detoxification seedlings of gerbera, can solve the problems of cumbersome operation and incomplete detoxification, and achieve the effect of simple process, easy operation and overcoming more detoxification materials

Inactive Publication Date: 2015-12-23
WUXI NANLIGONG TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to address the deficiencies in the existing gerbera detoxification technology, and provide a sustainable, easy-to-operate and large-scale production method for efficiently obtaining gerbera chrysanthemum detoxification seedlings. The technical detoxification is not complete and the operation is cumbersome, which can meet the needs of factory detoxification production

Method used

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  • Method for efficiently acquiring gerbera jamesonii detoxification seedling
  • Method for efficiently acquiring gerbera jamesonii detoxification seedling
  • Method for efficiently acquiring gerbera jamesonii detoxification seedling

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Embodiment 1

[0045] A method for efficiently obtaining virus-free seedlings of gerbera, the detailed steps of the method are as follows:

[0046] 1) Collect young leaves of gerbera variety Sunshine Coast, dedifferentiate to produce callus: collect healthy and disease-free young leaves of gerbera that have grown to the budding stage, wash with tap water, rinse with running water for 30 minutes, soak in 70% alcohol for 15 seconds , add a few drops of Tween-20 solution to 0.1% mercuric chloride, soak for 15 minutes, stir with a glass rod, rinse with sterile water for 5 times, blot dry with filter paper, punch holes with a hole diameter of 4mm, and inoculate the leaves Put on the callus induction medium (the formula of callus induction medium is: MS+2.0mg / L6-BA+0.5mg / LNAA+0.3g / LCH+3% sucrose+6.0g / L agar, pH is 5.8) on 27 Cultivate in dark at ℃, after 18 days we observed that light green rice-like callus was produced, such as figure 1 shown;

[0047] 2) Heat-treat the callus, and then contin...

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Abstract

The invention discloses a method for efficiently acquiring a gerbera jamesonii detoxification seedling. The method is characterized by comprising the following steps: (1) collecting gerbera jamesonii tender leaves, and de-differentiating the gerbera jamesonii to generate callus; (2) thermally treating the callus, continuously inducing the callus to form adventitious bud, and rooting the adventitious bud to form a strong tissue culture seedling; (3) further stripping a tissue culture seedling stem tip, and de-differentiating to generate callus; (4) thermally treating the callus, and inducing to form a seedling; (5) randomly extracting the tissue culture seedling in the step (4), carrying out the virus detection, if the seedling is qualified in detection, the seedling is the gerbera jamesonmii detoxification seedling, if the seedling is not qualified in the detection, repeating the steps (1) to (4) until the qualified gerbera jamesonii detoxification seedling is acquired. The callus is generated by virtue of the twice de-differentiation, and then the callus is thermally treated and de-differentiated to finally obtain the gerbera jamesonii detoxification seedling, so that the difficulty of the existing detoxification technology such as non-thoroughness in detoxification, complexity in operation and can meet the factory-like detoxification production requirement.

Description

technical field [0001] The invention relates to a method for efficiently obtaining virus-free gerbera seedlings, in particular to a method for producing callus tissue through twice dedifferentiation, heat-treating the callus tissue, and then differentiating the callus tissue to finally obtain virus-free seedlings of gerbera chrysanthemum efficiently. Background technique [0002] Gerbera ( Gerbera jamesonii Bolus), also known as gerbera and sunflower, is a perennial herb of the Compositae family. It is native to the Devasland region of South Africa, and then introduced to the UK. Now it is widely cultivated all over the world. Gerbera likes warm winters and cool summers. The flowers are large, delicate and upright, elegant and elegant, colorful but not coquettish, as beautiful as clouds, charming and elegant, refreshing and pleasing to the eye, giving people a sense of chic, soft, tranquil and serene. Gerbera is often used to cut flowers and place in vases. If it is paired ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 不公告发明人
Owner WUXI NANLIGONG TECH DEV
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