Cytoplasmic transfer to de-differentiate recipient cells

a technology of recipient cells and cytoplasm, which is applied in the direction of non-embryonic pluripotent stem cells, drug compositions, and fused cells, can solve the problems that the cells that form the placenta do not support the nuclear development and the cellular changes cannot be reversed, so as to achieve the effect of preventing senescence or reducing the number of cells

Inactive Publication Date: 2013-04-25
CHAPMAN KAREN B
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Benefits of technology

[0050]“Senescence”—In the present invention this refers to the characteristic slowing of growth of non-immortal somatic cells in tissue culture after cells have been maintained in culture for a prolonged period. Non-immortal cells characteristically have a defined life-span before they become senescent and die. The present invention alleviates or prevents senescence by the introduction of cytoplasm from a donor cell, typically an oocyte or blastomere, into a recipient cell, e.g., a cultured human somatic cell.

Problems solved by technology

For example, it was found that trophectodermal cells (the cells that form the placenta) did not support development of the nuclear fusion to the blastocyst stage.
Moreover, it was believed that these cellular changes could not be reversed.

Method used

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Embodiment Construction

[0051]As explained above, the present invention provides novel methods for de-differentiating and / or altering the life-span of desired cells, preferably mammalian cells and, most preferably, human or other primate cells by the introduction of cytoplasm from a more primitive cell type, typically an undifferentiated or substantially undifferentiated cell, e.g., an oocyte or blastomere.

[0052]As noted previously, it was recently reported in the popular press that a group working in the area of artificial insemination and infertility successfully transferred the cytoplasm from the oocyte of a younger woman into that of an older woman and thereby rejuvenated the ability of the older oocyte to be competent for fertilization and embryo development. Based on this anecdotal evidence, coupled with recent papers in the scientific literature which suggest that differentiated adult cells may be effectively “reprogrammed” by nuclear transfer, it was theorized that differentiated cells could be eff...

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Abstract

Methods for de-differentiating or altering the life-span of desired “recipient” cells, e.g., human somatic cells, by the introduction of cytoplasm from a more primitive, less differentiated cell type, e.g., oocyte or blastomere are provided. These methods can be used to produce embryonic stem cells and to increase the efficiency of gene therapy by allowing for desired cells to be subjected to multiple genetic modifications without becoming senescent. Such cytoplasm may be fractionated and / or subjected to subtractive hybridization and the active materials (sufficient for de-differentiation) identified and produced by recombinant methods.

Description

CROSS REFERENCE TO RELATED APPLICATIONS[0001]This application is a continuation-in-part of International Patent Application No. PCT / US00 / 18063, filed Jun. 30, 2000, which claims priority from U.S. Provisional Application Ser. No. 60 / 141,250, filed Jun. 30, 1999.FIELD OF THE INVENTION[0002]The present invention relates to methods for “de-differentiating” and / or altering the life-span of desired recipient cells, preferably human somatic cells. These methods have application especially in the context of cell therapies and the production of genetically modified cells.BACKGROUND OF THE INVENTION[0003]Nuclear transfer first gained acceptance in the 1960's with amphibian nuclear transplantation. (Diberardino, M. A. 1980, “Genetic stability and modulation of metazoan nuclei transplanted into eggs and ooctyes”, Differentiation, 17-17-30; Diberardino, M. A., N. J. Hoffner and L. D. Etkin, 1984; “Activation of dormant genes in specialized cells”, Science, 224:946-952; Prather, R. S. and Robl, ...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C12N15/873A61K35/12A01K67/027A61K35/54A61K48/00A61P43/00C12N5/074C12N5/10C12N5/16C12N9/12C12N15/07C12N15/09C12N15/12C12N15/16C12N15/18C12N15/19C12N15/79
CPCA61K35/12A61K48/00C12N5/0696C12N5/16C12N9/1241C12N2517/10C12N15/873C12N2503/00C12N2506/00C12N2510/04C12N15/79A61P43/00C12N5/0602C12N5/0606C12N2501/00C12N5/0607C12N2501/727C12N2510/00
Inventor CHAPMAN, KAREN B.
Owner CHAPMAN KAREN B
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