7 results about "Scutellarein" patented technology

The invention relates to a KASP molecular marker associated with scutellarin of erigeron breviscapus and application of the KASP molecular marker, and belongs to the technical field of genetic engineering. The primers of the KASP molecular marker associated with the scutellarin of the erigeron breviscapus comprise a forward primer 1, a forward primer 2 and a common reverse primer; the nucleotide sequence of the forward primer 1 is as shown in SEQ ID NO. 1; the nucleotide sequence of the forward primer 2 is as shown in SEQ ID NO. 2; the nucleotide sequence of the common reverse primer is as shown in SEQ ID NO.3. By utilizing the KASP technology, the SNP site and the corresponding primer combination provided by the invention not only improve the plant variety selection efficiency, but also are beneficial to shortening the breeding cycle and accelerating the cultivation of high-quality plant varieties. The markers have good genetic stability and high resolution, are suitable for high-throughput detection, and have significant application value in the field of plant breeding.

The invention relates to the technical field of antigen preservation, in particular to a preservation solution for improving the stability of an influenza A and B virus recombinant antigen, which comprises a buffer solution, water-soluble sugar, a protein stabilizer, a silymarin compound, a nonionic surfactant and a preservative, the antigen preserving fluid can effectively solve the problems of denaturation, aggregation and the like possibly occurring in the storage process of the influenza A virus recombinant antigen and the influenza B virus recombinant antigen, meets the requirement for long-term stable preservation of the influenza A virus recombinant antigen and the influenza B virus recombinant antigen, and is wide in application prospect.

The invention relates to the technical field of gene engineering, in particular to a product and a method for improving the yield of breviscapine in a plant body and a construction method of a tobacco synthetic biological chassis of breviscapine. The invention provides an application of any one or more gene sequences as shown in SEQ ID NO.1-SEQ ID NO.6 in improving the yield of breviscapine in a plant. The application comprises the following steps: connecting any one or more gene sequences as shown in SEQ ID NO.1-SEQ ID NO.6 with a pCAMBIA1302 vector to obtain a recombinant expression vector; transforming the recombinant expression vector into agrobacterium to obtain recombinant bacteria; a bacterial suspension of the recombinant bacteria is utilized to infect a plant to obtain a high-yield breviscapine plant, the yield of scutellarin in an obtained transgenic tobacco plant reaches 42.09 mu g / g DW, and a foundation is laid for efficient heterologous synthesis of breviscapine in tobacco.

The invention relates to the technical field of biology. The invention discloses a milk replacer capable of replacing calf colostrum and a feeding mode, and aims to reduce diarrhea of newborn calves and improve the survival rate of the newborn calves. The milk replacer contains traditional Chinese medicine extracts such as milk powder, concentrated whey protein, high-protein whey powder, cream, bovine colostrum powder, vitamin A, vitamin D3, lysozyme, saccharomyces cerevisiae boulardii, IGF, EGF growth factors, astragalus polysaccharide, chlorogenic acid and baicalin, and a feeding mode is provided. Many calves after birth cannot eat colostrum or cannot eat high-quality colostrum, the immunity of the calves is low, and the diarrhea rate is high. The product contains nutritional ingredients equivalent to calf colostrum, and through a reasonable feeding mode, passive immunity is established for newly born calves, diarrhea of the calves is reduced, the survival rate of the calves is increased, and good growth performance is obtained.

The present application is suitable for Scutellarein detection technical field, especially relates to a method for detecting the content of Scutellarein in traditional Chinese medicinal materials, comprising the following steps: step S1: selecting a predetermined amount of the to-be-tested traditional Chinese medicinal material, crushing it, mixing it with a predetermined amount of quartz sand, and then using a first pre-set concentration of ethanol solution for first extraction to obtain a preliminary extraction solution; step S2: performing solid-liquid separation on the preliminary extraction solution to separate out the ethanol extraction solution and retain the dregs; step S3: weighing a certain amount of the dregs, using a second pre-set concentration of ethanol solution for second extraction, and taking a proper amount of the second extraction solution as a sample solution. The present application realizes efficient and accurate detection of Scutellarein by optimizing the extraction conditions and the liquid chromatography detection steps. First, 70% ethanol solution is used for first extraction, which effectively dissolves Scutellarein in the traditional Chinese medicinal material, reduces impurity interference, and improves the purity of the preliminary extraction solution.

The application discloses a scutellarein derivative and application thereof in medicine. The compound of formula I or pharmaceutically acceptable salt thereof is an effective IGF2BP2 small molecule inhibitor, has obvious inhibitory activity on IGF2BP2, inhibits RNA reader at the proteinase level, and modifies and regulates m 6 A level, and can be used for treating diseases related to IGF2BP2, such as breast cancer, colorectal cancer, pancreatic cancer, hepatocellular carcinoma, acute myeloid leukemia, T-cell acute lymphoblastic leukemia, nasopharyngeal carcinoma and various tumors.

The application discloses a plant growth regulating composition containing scutellarein and seminole, and belongs to the technical field of agricultural plant growth regulators. The mass ratio of scutellarein and seminole in the effective component of the plant growth regulating composition provided by the application is 1:30-13:1. It is proved that scutellarein and seminole have a synergistic effect when used for promoting crop growth and improving crop yield within the range of the ratio, and are particularly beneficial to improving the quality of crop fruits.