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Isolated culture and extraction of plant stem cells of tomato and preparation method of oral liquids, beverages and white spirit

A plant stem cell, separation and culture technology, applied in the field of tomato plant stem cell separation and culture extraction and preparation of oral liquid, beverage and liquor, can solve the problems of cell line cell variation and so on

Inactive Publication Date: 2013-09-25
林树芳
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Even during such a dedifferentiation process, there will be bad signs of cell line cell variation due to chromosomal variation

Method used

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  • Isolated culture and extraction of plant stem cells of tomato and preparation method of oral liquids, beverages and white spirit
  • Isolated culture and extraction of plant stem cells of tomato and preparation method of oral liquids, beverages and white spirit
  • Isolated culture and extraction of plant stem cells of tomato and preparation method of oral liquids, beverages and white spirit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0095] Embodiment 1: the preparation of isolated material

[0096] (1) Tomatoes cultivated with pure farmyard manure without chemical fertilizers and pesticides are preferred. The skin is red, the inside is not yet ripe, and the skin is not scratched. Wash the surface attachments with water, and put 120mg / L L-ascorbic acid solution soak for 1 minute, then sterilize in 1% benomyl solution for 10 minutes, rinse with sterile water for 10 minutes, put in 75% ethanol for 1 minute, rinse with sterile water and put Sterilize in 30% hydrogen peroxide solution for 10 minutes, then rinse with sterile water for 10 minutes.

Embodiment 2

[0097] Embodiment 2: the preparation method of preferred solid medium:

[0098] (1) Potassium nitrate 2500mg / L, magnesium sulfate 122mg / L, manganese sulfate 11mg / L, zinc sulfate 2.5mg / L, copper sulfate 0.025mg / L, ammonium sulfate 135mg / L. Calcium chloride 112mg / L, potassium iodide 0.7mg / L, cobalt chloride 0.025mg / L, sodium dihydrogen phosphate 132mg / L, boric acid 2.6mg / L, sodium molybdate 0.23mg / L, niacin 2.0mg / L, Pyridoxine 2.1mg / L, L-Ascorbic Acid 53mg / L, Citric Acid 78mg / L, L-Aspartic Acid 140mg / L, L-Arginine 180mg / L, Glycine 75mg / L, Proline 120mg / L L. a-Naphthylacetic acid 2.2 mg. Sucrose 10.200mg / L. Activated carbon 500mg / L. Agar 3800mg / L. Magnetized water 0.7 liters.

[0099] (2) Dilute each nutrient of inorganic salt with 20ml of magnetized water, combine vitamins into 50ml of sterile water for dilution, put all amino acids into 50ml of sterile water for dilution, and put auxin into 10ml of sterile water for dilution. Then inject the inorganic salts into 0.7 lite...

Embodiment 3

[0100] Example 3: Induction of isolated materials and separation and culture of cambium

[0101] (1) Cut the sterilized tomato fruit into eight sections with an inoculation knife, remove the outermost thin skin, remove the inner core, take out the endothelial layer of the outer skin and put it into a solid medium, and cultivate it in a controlled darkroom for 26 days. The culture temperature is 25±℃.

[0102] (2) Gently transfer the cambium cell line into another flask of the same culture medium with an inoculation spatula, and then expand and cultivate it for 20 days.

[0103] (3) Then transfer the enlarged cultured cell line population into a liquid medium, and cultivate in a dark room with a shaker, at 160 rpm and in an environment of 25±° C., and the passage time is set to 20 days.

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Abstract

The invention provides isolated culture and extraction of plant stem cells of tomato and a preparation method of oral liquids, beverages and white spirit. As stem cells from a plant cambium of tomato is in an undifferentiated state, during amplified culture cells are high in hereditary stability, strong in activity, great in harvest rate and the like. The oral liquids, the beverages and the plant stem cell wine prepared by ethanol ultrasonic extraction have better use to resist cellular oxidation, prevent differentiated cells from turning to tumor cells, and reduce blood fat, blood pressure, cholesterol and e blood sugar.

Description

technical field [0001] The invention relates to a preparation method of tomato plant stem cell separation culture extraction and oral liquid, beverage and white wine. Background technique [0002] The present invention, as a plant stem cell isolation and culture method in bioengineering technology, is evaluated by the world's biological community as the most ideal technology that does not cause environmental problems and can be stably supplied as a plant source as an antioxidant substance added to various foods. According to Korean published patents: 1995-0000870 (1995.01.03); 2008801112097 (2008.10.10); 2010800289233 (2010.05.26). [0003] Extracting valuable substances from various plant cell lines has many advantages compared with direct extraction from plants, especially unlike previous extraction methods, it is not affected by the outside world, and can be sustainably cultivated and grown in large quantities. production, is considered the best solution to the destructi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/04C12G3/04A23L1/29A23L2/00A23L33/00
Inventor 林树芳
Owner 林树芳
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