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Antioxidant, anti-inflammatory, or anti-aging composition containing a plant stem cell line derived from taxus cambium or procambium as an active ingredient

一种化妆品组合物、形成层的技术，应用在抗炎剂、药物组合、植物细胞等方向，能够解决刺激损伤粘膜等问题

Active Publication Date: 2011-06-15
SHENZHEN LUQUAN IND CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For normal people, the inflammatory response removes the external source of infection, regenerates damaged tissue, and restores body function, but when the antigen is not removed or the inflammatory response occurs excessively or continuously due to endogenous substances, the immune response Irritation damages mucous membranes and, as a result, in some cases causes diseases such as cancer

Method used

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  • Antioxidant, anti-inflammatory, or anti-aging composition containing a plant stem cell line derived from taxus cambium or procambium as an active ingredient
  • Antioxidant, anti-inflammatory, or anti-aging composition containing a plant stem cell line derived from taxus cambium or procambium as an active ingredient
  • Antioxidant, anti-inflammatory, or anti-aging composition containing a plant stem cell line derived from taxus cambium or procambium as an active ingredient

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Example 1: Preparation of cell lines from taxus cambium or procambium.

[0062] (1) Preparation of plant material Shoots and stems of yew were collected separately, and then immediately immersed in 100 mg / L of antioxidant L-ascorbic acid (DUCHEFA, TheNetherlands). Then transfer it and store it away.

[0063] Then, with 1% benomyl (Dongbu Hannong Chemical, Korea), 1% chlorothalonil (Dongbu Hannong Chemical, Korea), 1% streptomycin sulfate (DUCHEFA, The Netherlands) and 0.1% cefotaxime The plants were pretreated with a mixed solution of sodium (DUCHEFA, The Netherlands) for 24 hours, followed by a 30-minute tap water rinse to remove phenolic compounds and residual chemicals. Next, the plants were sterilized in 70% ethanol (DCChemical, Korea) for 1 minute, in 30% hydrogen peroxide (LG Chemical, Korea) for 15 minutes, in 1% CLOROX solution for 15 minutes, and Disinfect surfaces in 3% CLOROX solution for 5 minutes, then wash 3-4 times with water.

[0064] (2) Separation...

Embodiment 2

[0073] Example 2: Preparation of cell line extracts from procambium or cambium.

[0074] (1) Preparation of DMSO (dimethyl sulfoxide) extract (i) 500 g of the cell line from which the medium was removed was dissolved in 500 ml of DMSO, and stirred at 50° C. for 6 hours.

[0075] (ii) After the lysis was completed, the cell solution was centrifuged at 3000 g for 10 minutes, and then the supernatant was collected to obtain a DMSO-soluble substance.

[0076] (iii) Concentrate the obtained DMSO-soluble material using a rotary vacuum concentrator.

[0077] (iv) The concentrated sample was dried using a freeze dryer, thereby obtaining a DMSO extract.

[0078] (2) The preparation of distilled water extract, methanol extract and acetone extract uses the cell line prepared in Example 1 to extract the active substance step by step as follows ( figure 2 ): (i) 500 g of the cell line from which the medium was removed was dissolved in 500 ml of distilled water, and stirred at 50° C. for...

Embodiment 3

[0086] Example 3: Culture of human diploid fibroblasts (HDF).

[0087] HDF cells were isolated from fetal penile foreskin and cultured. 10% fetal bovine serum (FBS, Hyclone, Logan, Utah, USA), 100 units / ml penicillin, 100 μg / ml streptomycin and 300 μg / ml glutamine to prepare the medium. The cells were incubated in the culture medium described above under 5% CO 2 They were cultured in an incubator at a temperature of 37° C. and a humidity of 95%, and subcultured at intervals of 3 to 4 days just before the cells were to fuse with each other. Subcultured cells are divided into young cells cultured for less than 20 generations, middle-aged cells cultured for 21-49 generations and aged cells cultured for more than 50 generations according to the number of subcultures (generations). The cultured cells were used in the experiments of Examples 4 to 6.

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Abstract

The present invention relates to an antioxidant, anti-inflammatory, or anti-aging composition containing one or more cell lines derived from Taxus cambium or procambium, an extract thereof, a lysate thereof and a medium thereof. The composition according to the present invention is useful for preventing and delaying aging as it minimizes the side effects of known antioxidants and anti-inflammatory agents, reduces active oxygen in a cell by participating in celleular metabolic action, and reduces and guides signals related to aging. Moreover, the composition according to the present invention is useful as a composition for cosmetic preparations for skin whitening as the same exhibits a melanin synthesis inhibition effect.

Description

technical field [0001] The present invention relates to an anti-oxidative, anti-inflammatory or anti-aging composition containing any one or more cell lines derived from taxus cambium or procambium, its extract, its lysate and its culture medium. Background technique [0002] Aging is a functional, structural and biochemical process that occurs continuously throughout human life. Aging occurs in all human cells and tissues, manifested as a decrease in metabolic rate, an increase in disease, a decrease in adaptability, etc., and eventually leads to the death of cells and the entire body tissue. Theories explaining the aging process and causes are largely divided into genetic theory (Chung.H.Y. et al., Kor.J.Gerontol., 2:1, 1992) and cell loss theory (Chung.H.Y. et al., Kor.J.Gerontol., 2 : 1, 1992). The theory of cellular attrition states that the cells of the body lose their function over time or due to the accumulation of harmful substances. Among these explanations, the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/13
CPCA61Q19/08A61K8/97A61K36/13A61Q19/02A61K8/9728A61K8/9755A61P3/00A61P17/00A61P17/18A61P29/00A61P39/06C12N5/04
Inventor 李大熙李恩景苏恩美陈荣雨
Owner SHENZHEN LUQUAN IND CO LTD
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