Method for improving chromium resistance and cadmium resistance of Festuca arundinacea with compost fermentation liquid
A fermented liquid, tall fescue technology, applied in botany equipment and methods, microorganism-based methods, biochemical equipment and methods, etc., can solve problems such as shortage
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Embodiment 1
[0099] (1) Isolation of Bacillus thuringiensis:
[0100] 1) Weigh 10g of a fresh compost sample, put it into a 90ml sterile water triangular flask with 20 glass beads, shake it, so that the microorganisms in the sample are fully and evenly distributed in the liquid, and use a pipette gun in the triangular flask Take 1ml of mother liquor, add it into a large test tube filled with 9ml sterile water and mix well, this is l0 -1 Concentration of the diluted solution, according to this method were diluted to l0 -2 , l0 -3 , l0 -4 , l0 -5 , l0 -6 Several concentrations of compost dilutions; use a pipette to draw 0.1 ml of concentration l0 -2 , l0 -3 , l0 -4 , l0 -5 , l0 -6 The dilution solution was injected into the solid medium of beef extract peptone plate, and each dilution was repeated three times, and sterile water was used as the blank control; the bacterial suspension was evenly spread on the solid medium of whole beef extract peptone with a sterile glass spatula, s...
Embodiment 2
[0114] 1) Preparation of Bacillus thuringiensis fermentation broth:
[0115] A. Purify and cultivate the purchased Bacillus thuringiensis for 2 generations; then insert it into a 250 mL Erlenmeyer flask containing 50 mL of beef extract peptone liquid medium, cultivate at 180 r / min at 37°C, and use 600 nm wavelength for turbidimetric measurement to determine The OD value of the bacterial suspension is the ordinate, and the culture time is the abscissa, and the growth curve of the microorganism is drawn, and the time from the growth of Bacillus thuringiensis to the stationary phase is obtained according to the growth curve; the second-generation culture of the purified culture refers to: Pick up the colonies to be purified directly, and inoculate them into the solid medium of Gaoshi No. 1 plate prepared in advance, gently streak the plate from left to right, and place the culture plate upside down in a constant temperature incubator at 37°C for 1 Days, this process is the firs...
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