Nitrogen fixing and phosphate solubilizing growth-promoting bacteria for desert plants and microbial agent and application thereof

A technology for nitrogen-fixing bacteria and crops, applied in the field of microorganisms, can solve problems such as limited applications

Active Publication Date: 2018-04-06
GUIZHOU EDUCATION UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the efficiency of symbiotic nitrogen fixation by rhizobia is higher than that of combined nitrogen fixation, it has a high degree of host specificity, which limits its application in non-host leguminous forages and non-legume forages

Method used

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  • Nitrogen fixing and phosphate solubilizing growth-promoting bacteria for desert plants and microbial agent and application thereof
  • Nitrogen fixing and phosphate solubilizing growth-promoting bacteria for desert plants and microbial agent and application thereof
  • Nitrogen fixing and phosphate solubilizing growth-promoting bacteria for desert plants and microbial agent and application thereof

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Embodiment 1

[0063] Example 1, microbial nitrogen-fixing bacteria, whose classification is named as Microbacterium trichothecenolyticum SPJ01, is preserved in the China Center for Type Culture Collection, the strain preservation number is CCTCC NO: M2016254, the preservation date is May 9, 2016, and the preservation address is Wuhan, China. The microbial nitrogen-fixing bacterium provides nitrogen and phosphorus application to crops in soil, and relieves growth inhibition caused by nitrogen and phosphorus deficiency.

[0064] Microbial nitrogen-fixing bacteria were prepared as follows to provide nitrogen and phosphorus to crops: Microbacterium SPJ01 stored at 4°C was activated for 2 hours and then placed in a YMA liquid medium, shaken at 120r / min at 28°C Centrifuge at 10,000 r / min for 10 minutes until the absorbance value at 665nm of the bacterial solution is ≥0.8, wash the bacterial cells with sterile water and shake them up to disperse them to make a bacterial suspension with an OD665nm v...

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Abstract

The invention discloses microorganism nitrogen-fixing bacteria. The microorganism nitrogen-fixing bacteria is classified and named as microbacterium trichothecenolyticum SPJ01, and is conversed in China Center for Type Culture Collection, the culture conversation number is CCTCC NO:M2016254, and the conservation date is May 6 th, 2016; the application of the microorganism nitrogen-fixing bacteriain soil provides nitrogen elements and phosphorous elements for crops, and relieves the inhibition of growth due to nitrogen deficiency and phosphorous deficiency; the microorganism nitrogen-fixing bacteria serves as a nitrogen-fixing growth-promoting microbial agent in a desert area. The growth inhibition of the crops under the condition of nitrogen deficiency and phosphorous deficiency can be relieved; meanwhile, the crops have an obvious promoting effect on the growth of crop seedlings, and various growth indexes of two kinds of forage like the plant height, the root length, the single leafarea, the single plant leaf number, the root size, the plant size, the overground fresh dry weight, the underground fresh dry weight, the plant fresh dry weight, the root shoot ratio (R / T) and the dry fresh weight (D / F) are all obviously improved.

Description

technical field [0001] The invention relates to a nitrogen-fixing, phosphorus-decomposing, growth-promoting bacterium that can be used for desert plants, a bacterial agent and its application, and belongs to the technical field of microorganisms. Background technique [0002] As essential mineral elements for plants, nitrogen and phosphorus are important components of various structural and functional substances such as proteins, nucleic acids, phospholipids, enzymes, and plant hormones in plant living cells. It plays an important role in the process of gene expression and regulation and cell signal transduction. The lack of nitrogen and phosphorus will inhibit the growth and development of plants, cause some deficiencies, and even fail to complete their life cycle. Long-term large-scale application of chemical fertilizers not only increases the cost of agricultural production, but also causes the selective accumulation of certain elements in the soil and the deterioration ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F11/08C12R1/01
CPCC05F11/08C12N1/20C12N1/205C12R2001/01
Inventor 李剑峰张淑卿
Owner GUIZHOU EDUCATION UNIV
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