Nitrogen fixing and phosphate solubilizing growth-promoting bacteria for desert plants and microbial agent and application thereof
A technology for nitrogen-fixing bacteria and crops, applied in the field of microorganisms, can solve problems such as limited applications
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[0063] Example 1, microbial nitrogen-fixing bacteria, whose classification is named as Microbacterium trichothecenolyticum SPJ01, is preserved in the China Center for Type Culture Collection, the strain preservation number is CCTCC NO: M2016254, the preservation date is May 9, 2016, and the preservation address is Wuhan, China. The microbial nitrogen-fixing bacterium provides nitrogen and phosphorus application to crops in soil, and relieves growth inhibition caused by nitrogen and phosphorus deficiency.
[0064] Microbial nitrogen-fixing bacteria were prepared as follows to provide nitrogen and phosphorus to crops: Microbacterium SPJ01 stored at 4°C was activated for 2 hours and then placed in a YMA liquid medium, shaken at 120r / min at 28°C Centrifuge at 10,000 r / min for 10 minutes until the absorbance value at 665nm of the bacterial solution is ≥0.8, wash the bacterial cells with sterile water and shake them up to disperse them to make a bacterial suspension with an OD665nm v...
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