38 results about "Dactylis glomerata" patented technology

A method of preparing a dactylis glomerata root tissue paraffin section is disclosed. The method includes a step of killing and immobilizing a fresh dactylis glomerata root tissue block, a step of washing, a step of dehydrating and hyalinizing, a step of wax soaking and embedding, a step of block trimming and sectioning, a step of section expanding and roasting, a step of dewaxing, rehydrating and staining with safranine, a step of staining with fast green, dehydrating and hyalinizing and a step of section sealing. After the step of washing and before the step of dehydrating and hyalinizing, agarose pre-embedding is performed and includes embedding the washed dactylis glomerata root tissue block into agarose gel having a content of 5% by mass and melt by stewing. The dactylis glomerata root tissue paraffin section prepared by the method is complete in tissue, clear in cell boundary, bright in color, sharp in color contrast and clean in sectioning.

The invention discloses a mixed-sowing method of forage grass in a high altitude area of the Three Gorges reservoir area, comprising soil preparation before sowing, mixed sowing and fertilizer application, wherein the mixed sowing means mixed sowing of orchard grass seeds, trifolium repens seeds and alfalfa seeds, and specifically comprises the following steps of: performing row-spaced drilling on the orchard grass seeds and the alfalfa seeds, with row spacing of 0.3m; and uniformly sowing the trifolium repens seeds among the rows of the orchard grass seeds and the alfalfa seeds, wherein the dosage ratio of the orchard grass seeds to the trifolium repens seeds to the alfalfa seeds is 1: (1 to 1.6): (1 to 1.6), in parts by weight. The leguminous forage grass and gramineous forage grass selected for the mixed-sowing of forage grass disclosed by the invention are featured with heat resistance, drought resistance and leanness resistance, and are mixed-sowed with each other, thus adequately utilizing light energy and increasing photosynthates; additionally, the leguminous forage grass has a high protein content and the rhizobia of the leguminous forage grass has a function of nitrogen fixation, thus remarkably increasing the yield of the forage grass, and improving the quality of the forage grass and soil fertility, so that the advantages of the leguminous forage grass are very prominent. The method disclosed by the invention is simple and convenient, and simple in operation; and the artificial pasture established by the method has the characteristics of heat resistance, drought resistance and leanness resistance.

The invention discloses a method for establishing a dactylis glomerata tissue culture regeneration system. The season limitation of explant supply can be overcome, and the experimental period can be greatly shortened. Dactylis glomerata mature seeds serve as explants, after the dactylis glomerata mature seeds are cleaned and sterilized, an induction medium is inoculated with the dactylis glomerata mature seeds for culture for 26-30 d, calluses are obtained, the calluses are transferred into a callus subculture medium for culture for 5-9 d, the calluses are transferred into a differential medium for culture for 18-22 d, and when seedlings grow to 2-3 cm, the seedlings are transplanted. The efficient dactylis glomerata tissue culture regeneration system is established through the method, a foundation is laid for establishing a dactylis glomerata tissue efficient genetic transformation system, technical reference is provided for breeding good dactylis glomerata tissue varieties, the season limitation of explant supply is overcome, and the experimental period is greatly shortened. The method is suitable for being applied and popularized in the field of the biotechnology.

The invention discloses a method for controlling invasion of flaveria bidentis through ecological substitution. The method comprises the following steps: taking sorghum sudanese or sorghum sudanese and cocksfoot as the plant for controlling the invasion of the flaveria bidentis; and planting the sorghum sudanese or planting the sorghum sudanese and the cocksfoot in a hybrid way in an ecosystem which may be invaded easily by the flaveria bidentis so as to control the invasion of the flaveria bidentis. The sorghum sudanese is a species which can contend against the flaveria bidentis in a dry season and enjoy apparent advantage in the struggle for survival with the seedling of the flaveria bidentis. The sorghum sudanese can completely play a role in the ecosystem which can be easily invaded or has been invaded by the flaveria bidentis through introduction, planting, rational configuration and scientific management, so that the seedling of the flaveria bidentis can be effectively prevented from growing quickly in the dry season and occupying the space by using the effect of time difference; and consequently, the method provides basic protection to prevent the invasion of alien invasive species flaveria bidentis as an alternative and to perform sustainable management.

The invention discloses an agaricus bisporus culture medium and a preparation method thereof. The agaricus bisporus culture medium is prepared from the following component raw materials in parts by weight: 25-30 parts of waste cotton, 4-9 parts of tea residues, 20-25 parts of coconut bran, 10-15 parts of watermelon peel, 4-7 parts of egg shell powder, 10-15 parts of apple pomace, 4-8 parts of fishbone dust, 15-20 parts of dactylis glomerata, 10-15 parts of neyraudia reynaudiana, 28-35 parts of livestock manure, 3-6 parts of carbamide, 7-12 parts of leaf mould, 3-6 parts of bentonite, 1-4 parts of lime, 0.2-0.3 part of diammonium phosphate, 0.1-0.3 part of potassium sulfate and 5-10 parts of nutritional additives. The culture base material is wide in raw material source and rich in nutrients, enables the thalli to vigorously grow, increases the content of protein and vitamins, enhances the nutritional health value by absorbing the beneficial components of the nutritional additives and the watermelon peel, has the effects of reducing fever, causing diuresis and expelling toxins, is high in biological transformation rate, has good stress resistance and enhances the market value of agaricus bisporus culture.

The invention relates to a method for planting forage grass on a sunward side of hillside land with the slope greater than 15 degrees in an alpine mountainous area. The method comprises the steps thatthe forage grass is planted in a rotation place in the alpine mountainous area with the altitude of 2,000-2,500 m in the southwestern Yunnan province of China, the sunward-side hillside land with theslope greater than 15 degrees is selected as the rotation place, and after former crops are harvested, the forage grass is planted; forage grass planting is conducted by adopting the mode that 9-11%of annual ryegrass seeds, 18-22% of perennial ryegrass seeds, 9-11% of hybrid ryegrass seeds, 28-32% of dactylis glomerata seeds, 28-32% of trifolium repens seeds and base fertilizer are mixed and sowed, and grazing can be conducted when the forage grass grows till the height of a grass layer exceeds 5 cm; when the forage grass is surplus, the harvested forage grass is dried and prepared into green hay or silage, and the stubble height during grass harvesting is 5 cm. The method for planting the forage grass in the alpine mountainous area has the advantages that the method is suitable for being implemented in the southwestern Yunnan province of China, and the forage grass is quick in propagation, good in growth and significant in ecological restoration effect.

The invention discloses an optimized mixed sowing method for forage grass of grazing grassland and aims to increase land utilization rate and forage grass yield and improve economic efficiency throughmixed sowing of the forage grass. According to the technical scheme, the method comprises following steps: 1), rows are formed after basal fertilizer application and soil leveling; 2), vetch seeds, ryegrass, dactylis glomerata, broad beans and mustard seeds are screened; 3), the seeds are subjected to sowing in drill or broadcast sowing into rows, irrigation is performed after sowing, and seed beds keep sufficient soil moisture; 4), regular watering is performed after earth covering.

The invention discloses a Dactylis glomerata and trifolium repens mixed sowing method. The method comprises the following steps of (1) turning over a cultivated land and then flattening the land, (2) broadcasting the actylis glomerata and trifolium repens according to a weight proportion of 6 to 4 between the last ten-day of May to the last ten-day of June, (3) covering broadcast grass seeds via soil, (4) applying fertilizer, and (5) cutting after grass seeds sprout and between a tillering period and a jointing period, and then applying fertilizer again after the cutting. Perennial Gramineae plant actylis glomerata and perennial tleguminosae plant trifolium repens are combined to form different life form herbage mixed-sowed group; herbage output can be improved by the mixed-sowing method; meanwhile, herbage protein content can be improved; herbage palatability, soil structure and soil fertility can be improved; and strong instructive meaning can be provided for artificial grassland planting in southern areas.

The invention discloses a plant composition and a method for repairing side slopes of roads in southern high-altitude regions, and relates to the technical field of agriculture. The plant compositionfor repairing the side slopes of the roads in the southern high-altitude regions comprises tree and shrub plants, grass flower plants and herbaceous plants. The tree and shrub plants are selected froman optional type or a plurality of optional types of rhus chinensis, cassia glauca, buddleja lindleyana, robinia pseudoacacia, pinus massoniana and hibiscus syriacus; the grass flower plants are selected from an optional type or a plurality of optional types of perennial coreopsis, zinnia elegans, cosmos sulphureus and sanvitalia procumbens; the herbaceous plants are selected from an optional type or a plurality of optional types of lolium perenne, trifolium repens, dactylis glomerata and trifolium pratense. The method for repairing the side slopes of the roads in the southern high-altitude regions includes planting seeds of the plant composition on the side slopes. The plant composition and the method have the advantages that appropriate plant varieties are selected, accordingly, growthand development nodes of the plants can be staggered from one another, and nitrogen, phosphorus, potassium and nutrient competition of the plants can be relieved; seeds of diversified species are sownin a mixed manner, accordingly, intra-species competition and inter-species competition can be reduced, the survival rate of the plants can be increased, and formed landscape effects can be improved.

A fertilizing method for orchard grass is characterized in that urea is taken as nitrogen fertilizer, calcium superphosphate is taken as phosphate fertilizer, and potassium sulfate is taken as potash fertilizer, wherein the use amount of nitrogen, phosphorus and potassium follows the proportion that N:P205:K20 by weight portion is 1.986:1:2.114. The fertilizing method comprises the steps as follows: firstly, all the phosphate fertilizer, 45% of the nitrogen fertilizer and 45% of the potash fertilizer are taken as base fertilizer to be applied in cultivating soil, then 25% of the nitrogen fertilizer and 25% of the potash fertilizer are applied as the first additional fertilizer at the tillering stage, and the remaining 30% of the nitrogen fertilizer and the remaining 30% of the potash fertilizer are distributed averagely and applied after reaping of each time. According to the invention, suitable nitrogen fertilizer, phosphate fertilizer and potash fertilizer are selected and the nitrogen fertilizer, the phosphate fertilizer and the potash fertilizer are used in proper proportions, so that the nutrition requirement of orchard grass can be well met, and meanwhile, the fertilizing manner is combined to fully improve nutrition absorption of orchard grass in the early and later growing periods; therefore, the quality of orchard grass can be improved effectively, the yield can reach59450.33 kg / hm<2>, the energy consumption is reduced, environment pollution is avoided, the soil fertility is improved, and the economic benefits in production of orchard grass can be increased greatly.

Provided is a preparation method of silage, wherein the silage is prepared by carrying out silage fermentation of mixed forage grass composed of 50%-60% of dactylis glomerata, 10%-20% of ramie and 20%-40% of Shuicheng festuca arundinacea and used as a raw material according to the weight percentage. The prepared mixed silage is yellow green, has obvious sour fragrant flavor, is not pungent, moist and compact, is easy to separate, has stems and leaves mostly maintaining the original state, and has clear and visible leaf veins; the silage has good palatability, the lactic acid content in the silage (fresh sample) is high, the pH value can reach 3.5, and the maximum pH is not more than 3.9; the loss of main nutrients of the silage is 6% or less, the main nutritional quality is close to that of silage raw materials, and the silage has good palatability, has ruminant animal feed intake, digestibility rate of organic matters and the effective energy value all similar to those of the silage raw materials, and has good nutrition quality.

High-quality silage is formed by carrying out silage fermentation of mixed forage grass composed of 50%-60% of dactylis glomerata, 20%-40% of setaria grass and 10%-20% of ramie according to the weight percentage. The prepared mixed silage is yellow green, has obvious sour and fragrant flavor, is not pungent, moist and compact, is easy to separate, has stems and leaves maintained with the original states, and has clear and visible leaf veins; the silage has good palatability, the lactic acid content in the silage (fresh sample) is high, the pH value can reach 3.8, and the maximum pH is not more than 4.2; the loss of main nutrients of the silage is 6% or less, the main nutritional quality is close to that of silage raw materials, and the silage has good palatability, has corresponding ruminant animal feed intake, digestibility rate of organic matters and the effective energy value all similar to those of the silage raw materials, and has good nutritional quality.

The invention provides a grass / white clover land degeneration degree grading method and application thereof and relates to the technical field of grassland agriculture. According to the grading method, grassland is divided into non-degraded grassland, low-grade degraded grassland, medium-grade degraded grassland and high-grade degraded grassland according to standards; particularly, grass / white clover land degeneration degree is graded by measuring annual dry matter yield of forage grass, dry matter components of sowed perennial ryegrass and orchardgrass, dry matter component of white clover,tillering density of sowed perennial ryegrass, tillering density of orchardgrass, stolon density of white clover, and pH of soil of grassland at the depth of 0-10cm. The grading method can complete grading of degeneration degree of grass / white clover land simply and practically.

The invention discloses a hybrid breeding method of cocksfoot, comprising the steps of: cultivating Himalaya cocksfoot subspecies; cultivating original cocksfoot subspecies; pollinating during the blossoming period of the cocksfoot of the Himalaya cocksfoot subspecies and the blossoming period of the cocksfoot of the original cocksfoot subspecies; storing the seeds in the inflorescence of the cocksfoot of the Himalaya cocksfoot subspecies; carrying out individual cultivation on the seeds; and determining the successfully hybridized individual as novel cocksfoot according to the morphological feature of the spikelet during the individual cultivation period. The invention also discloses a novel cocksfoot variety cultivated by the method.

The invention discloses a method for fertilizing orchardgrass. The method utilizes urea as nitrogenous fertilizer, calcium superphosphate as phosphate fertilizer and potassium sulfate as potassic fertilizer, wherein a weight part ratio of N to P2O5 to K2O is 1.986: 1: 2.114. The method comprises the following steps of applying all the phosphate fertilizer, 45% of the potassic fertilizer and 45% of the nitrogenous fertilizer as base fertilizer into cultivation soil, applying 25% of the nitrogenous fertilizer and 25% of the potassic fertilizer as primary topdressing fertilizer into the cultivation soil in a tillering stage, and averagely applying the rest of the nitrogenous fertilizer and the potassic fertilizer into the cultivation soil after each harvest process. Through selection of appropriate nitrogenous fertilizer, phosphate fertilizer and potassic fertilizer and reasonable collocation of the fertilizer use amounts, the method satisfies orchardgrass nutritional requirements, fully improves nutrient absorption in growth earlier and later stages of orchardgrass by the fertilization processes, effectively improves orchardgrass quality and output, reduces energy consumption, avoids environmental pollution, improves soil fertility, and greatly improves economic benefits of orchardgrass production.

The invention discloses a pasture planting method of a mountainous region with a slope of 10-25 DEG of a cold highland area. The method is that pasture planting is carried out in the mountainous region with the slope of 10-25 DEG of the cold highland area with an altitude of 2000-2500 m in the southwest of Yunnan Province, China; the pasture planting is that 9-11% of annual ryegrass Abundant seeds, 28-32% of perennial ryegrass Mathilde seeds, 28-32% of dactylis glomerata Donata seeds and 28-32% of white clover Riesling seeds are mixed sowed in a sunny face of the mountainous region; and 9-11%of annual ryegrass Abundant seeds, 28-32% of perennial ryegrass Mathilde seeds, 28-32% of dactylis glomerata Amba seeds and 28-32% of white clover supper Haifa seeds are mixed sowed in a shady face ofthe mountainous region. The invention provides the cold highland area pasture planting method suitable for implementation in the southwest of Yunnan Province, with rapid pasture propagation, good pasture growth, and significant ecological restoration effect.

The invention relates to the technical field of animal feeds and particularly relates to a silage feed and a preparation method thereof. By feeding animals on forage grass, animals can absorb the nutrients, but the nutrients in the forage grass cannot be completely decomposed and absorbed, which cause waste, since animal bodies are lack in some enzymes and bacteria. The invention provides the silage feed which includes, by weight, 100-300 parts of potato flour, 300-600 parts of corn flour, 1800-2000 parts of forage mulberry, 300-450 parts of dactylis glomerata, 200-600 parts of alfalfa grass,30-50 parts of urea, and 5-10 parts of dry powder of composite bacteria. The nutrients in the forage mulberry, dactylis glomerata and alfalfa grass can be decomposed by lactic acid bacteria, cellulosedegrading enzymes and bacillus subtilis in the dry powder of composite bacteria, so that the nutrients in the animal feed can be absorbed and utilized by animals easily.

The invention discloses a method for inducing generation of dactylis glomerata L.hairy roots by agrobacterium rhizogenes. The method comprises the following steps of (1) activating the agrobacterium rhizogenes; (2) performing infection; (3) inducing the dactylis glomerata L.hairy roots; (4) performing degerming and culturing; and (5) performing subculturing, so as to realize high efficiency induction and scaleable culture of the dactylis glomerata L.hairy roots. The production cycle is short, and the production cost is low.

The invention relates to a method for promoting seed germination. According to the invention, a penicillin solution is utilized to carry out seed soaking treatment on cocksfoot seeds, wherein the concentration of the penicillin solution is 100-500 mg / L, and the treatment time is 6-24 hours. The germination rate of the cocksfoot seeds, subsequent growth property and the like are obviously enhanced as compared with the conventional clear water seed soaking, the germinative force of the cocksfoot seeds can reach 75-78%, and the germination rate can reach 78-83%; and the germinated cocksfoot seeds, which are subjected to seed soaking in the penicillin solution, have favorable growth property.

A method for promoting the growth of orchardgrass includes the steps of directly conducting hill-seedling on one orchardgrass seed in each feeding block with a substrate, covering the upper layer of the seed with the corresponding substrate with the thickness of 1.0-1.2 cm, conducting gentle pressing, putting the feeding blocks in a seedling bed, adding water to the seedling bed until the water reaches the position, 1.5-1.8 cm above the bottom, of each feeding block and completely wets the corresponding substrate, supplementing water at any time before the seedlings emerge so that the water content of the substrates is 35-40%, transplanting seedlings in the three-leaf period into flowerpots to continue being cultured for one month, and regularly spraying a naphthylacetic acid solution. Theorchardgrass is high in growth vigor, plants are high, the plants can reach up to 17.26 cm or above after being processed for 21 days, the tillering number is high and can reach 4 or above, the biomass increase is high in the growth process, and the method is simple, reliable and worthy of application and market promotion.

The invention provides a method for promoting orchardgrass seed germination... The method comprises the step of adopting penicillin solution to carry out soaking treatment on orchardgrass seeds, wherein the concentration of the penicillin solution is 100mg / L-500mg / L and the treatment time is 6-24 hours. In comparison with the ordinary seed soaking using clean water, the germination percentage, the follow-up growth performance and the like of the orchardgrass seed are improved significantly; the germinative force of the orchardgrass seed can be 75-78%; the germination percentage can be 78-83%;and the orchardgrass seed subjected to seed soaking using the penicillin solution is good in growth performance after germination.

The invention relates to a simple and effective method for promoting seed germination. According to the invention, a penicillin solution is utilized to carry out seed soaking treatment on cocksfoot seeds, wherein the concentration of the penicillin solution is 100-500 mg / L, and the treatment time is 6-24 hours. The germination rate of the cocksfoot seeds, subsequent growth property and the like are obviously enhanced as compared with the conventional clear water seed soaking, the germinative force of the cocksfoot seeds can reach 75-78%, and the germination rate can reach 78-83%; and the germinated cocksfoot seeds, which are subjected to seed soaking in the penicillin solution, have favorable growth property.

The invention provides a cultivation method for improving the summer-over rate of dactylis glomerata. The method comprises the steps of matrix preparation, seeding, treatment after germination, transplanting and the like. By means of the method, the dactylis glomerata can quickly grows in the planting process, the plant height is quickly increased, 14 days after treatment cultivation, the plant height can reach 48.2 centimeters, the leaf length is quickly increased, and 14 days after cultivation, the leaf length can reach 43 centimeters; the leaf width is quickly increased, and 14 days after cultivation, the leaf width can reach 0.7 centimeter. The tiller number is large and can reach 5; the chlorophyll content is high and can be up to 46.72 mg / g; the summer-over rate is high. The method is simple, reliable and worthy of market application and popularization.

The invention discloses a traditional Chinese medicine preparation for treating whooping cough. The Chinese medicinal preparation for treating whooping cough is mainly prepared from the following raw medical materials in parts by weight: 10-16 parts of radix curcumae, 7-12 parts of caudate sweetleaf flower, 8-13 parts of Chinese ephedra, 10-14 parts of aster fastigiatus, 12-16 parts of mulberry leaves, 10-13 parts of herbof paniculate spotflwoer, 9-15 parts of flower of Chimonanthus praecox Link, 7-13 parts of Polygonum chinense, 10-16 parts ofliquorice, 7-10 parts of air potato, 10-15 parts of small centipeda herb, 6-11 parts of herb of diversifolious pimpinella, 10-16 parts of goldthread, 9-13 parts of herb of ceylon leucas, 11-14 parts of winter cherry, 10-17 parts of dactylis glomerata, 8-12 parts of arcangelisia loureiri and 6-13 parts of tabasheer. The traditional Chinese medicine preparation for treating whooping cough is same in material selection, low in drug cost and free of any toxic and side effects. As multiple medical materials are matched rationally to play medicinal effects cooperatively, the traditional Chinese medicine preparation for treating whooping cough has the effects of clearing heat and dissipating phlegm, ventilating the lung and arresting cough, and the like, is good in curative effect, high in curing rate and short in course of treatment, and can treat both manifestation and root cause of whooping cough.

Provided is a breeding method of goats. The breeding method of the goats includes the following steps that lolium perenne, dactylis glomerata, wheat and secale cerale are planted in blocks of a breeding field; a goat house keeps well ventilated, global warming equipment is arranged, and sterilization is conducted periodically; in an earlier stage of fattening, the goats are fed with, by weight part, the wheat, soybeans, sweet potato powder, fish meal, white granulated sugar, edible salt, potassium iodate and citric acid; in a medium stage of fattening, the goats are fed with, by weight part, corn, rice bran, taro powder, bean pulp, rape seed cakes, bone meal, vitamin complex, sodium citrate and edible essence; in a later stage of fattening, the goats are fed with, by weight part, rice, bran, potato powder, bean pulp, urea, calcium hydrogen phosphate, edible salt and sodium carboxymethylcellulose. By adopting the breeding method of the goats, from characteristics of the growth and fattening stages of the goats, different nutrient elements are reasonably and evenly provided for the goats, it is ensured that the goats flesh up evenly in the fattening stages, the fat content of muttonof the goats is reduced, the nutrient elements of the mutton of the goats are ensured, and it is guaranteed that the mutton of the goats is fresh, tender, good in mouthfeel, light in smell of mutton and high in nutrition value.