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A method of preparing a dactylis glomerata root tissue paraffin section

A paraffin section and root technology, which is applied in the field of paraffin section of Duck grass root tissue, can solve the problems of many chemical reagents, long production cycle and high production cost, and achieve the effects of clear cell boundaries, bright color contrast and good test effect.

Inactive Publication Date: 2017-02-15
SOUTHWEST UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the traditional paraffin section method has a long production cycle and is easily restricted by conditions. There are many chemical reagents used in the production, which is poisonous to humans, and it is easy to make the material brittle, hard or deformed. It is easy to disappear, often makes the tissue smaller, or even twists and deforms the tissue, the substance contained in the cell shrinks and the refraction is enhanced, which affects the accuracy of observation, and the production cost is high. How to quickly and accurately embedding is also the key to film production

Method used

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  • A method of preparing a dactylis glomerata root tissue paraffin section
  • A method of preparing a dactylis glomerata root tissue paraffin section
  • A method of preparing a dactylis glomerata root tissue paraffin section

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Embodiment 1

[0048] A preparation method for the paraffin section of the root tissue of Dactylis chinensis, according to the following steps:

[0049] (1) kill and fix

[0050] Cut the lateral root of fresh 'North Dian' Dactylis chinensis, and use a disposable double-sided blade to cut out the root tissue of Dactylis chinensis, about 2-3 mm, and immediately place it in the FAA mixed fixative for fixation, and the FAA mixed fixative Formalin, glacial acetic acid, and 70% ethanol with a volume ratio of 1:1:18; the volume of the fixative should be 10 to 15 times the total volume of the root tissue block of D. chinensis. After the material is fixed, if it is not used in time, it needs to be preserved for later use; the root tissue of the duckweed can be stored in the above-mentioned FAA mixed fixative solution and placed in the refrigerator at 4°C for half a year to one year, and the structure of the material will not change or deteriorate . The killing and fixation effectively prevent the a...

Embodiment 2-4

[0074] The first roots of ‘PI 594994’ Dactylis chinensis, the first roots of ‘PG 49’ Dactylis chinensis, and the first roots of ‘Qiancao No. 4’ Dactylis chinensis were used to make slices respectively. Others are the same as the method of embodiment 1, and the results are as follows Figure 2-4 shown. Such as image 3 Arrows: Older primary roots or visible epidermal cells keratinized, appearing as bright green "fish scales". Figure 4The lignification and suberinization of the cell walls of the outer cortical cells can be seen at the arrow, and the inner cortical cells are "horseshoe-shaped". The tissue is intact, the sections are clean, the cell boundaries are well defined, and the colors are bright and contrasting.

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Abstract

A method of preparing a dactylis glomerata root tissue paraffin section is disclosed. The method includes a step of killing and immobilizing a fresh dactylis glomerata root tissue block, a step of washing, a step of dehydrating and hyalinizing, a step of wax soaking and embedding, a step of block trimming and sectioning, a step of section expanding and roasting, a step of dewaxing, rehydrating and staining with safranine, a step of staining with fast green, dehydrating and hyalinizing and a step of section sealing. After the step of washing and before the step of dehydrating and hyalinizing, agarose pre-embedding is performed and includes embedding the washed dactylis glomerata root tissue block into agarose gel having a content of 5% by mass and melt by stewing. The dactylis glomerata root tissue paraffin section prepared by the method is complete in tissue, clear in cell boundary, bright in color, sharp in color contrast and clean in sectioning.

Description

technical field [0001] The invention relates to the technical field of paraffin sectioning, in particular to a method for paraffin sectioning the root tissue of Dactylis chinensis. Background technique [0002] The paraffin section method uses paraffin as an embedding agent and undergoes a series of treatments to make permanent slices. It can not only cut the material into extremely thin slices (below 4 μm), but also make continuous slices and store them permanently with good clarity, and is also suitable for cytological research. Paraffin section is the most important and most commonly used method in plant microscopy, and it is widely used in the production and scientific research of plant anatomy and related fields. [0003] Especially in recent years, with the development of science and technology and the rise of plant ecological anatomy, paraffin section has become an important means for the study of ecological adaptability of plants in different habitats or succession ...

Claims

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Application Information

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IPC IPC(8): G01N1/28
CPCG01N1/28
Inventor 曾兵吴佳海王少青陈思怀牟琼张文李娟
Owner SOUTHWEST UNIVERSITY
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