6 results about "Chimonanthus" patented technology

The application discloses the technical field of molecular identification of plant species, and discloses molecular markers for identifying five species of Chimonanthus and application of the molecular markers. The application develops specific molecular markers (cox2i691 and nad4i1399) based on variable regions between mitochondrial genomes of Chimonanthus praecox and Chimonanthus nitens, and effectively distinguishes the five species of Chimonanthus, i.e. Chimonanthus praecox, Chimonanthus nitens, Chimonanthus salicifolius, Chimonanthus zhejiangensis and Chimonanthus oblongus. The application is beneficial to conveniently and accurately identifying the species of Chimonanthus, and provides important information for analyzing evolution of the Chimonanthus and analyzing a forming mechanism of biochemical properties in the future, and is also beneficial to classification of the species of Chimonanthus, evolution research of mitochondrial genomes, and product discrimination of the Chimonanthus species.

The invention relates to a chimonanthus praecox calmodulin protein CpCML25 and an application thereof. The CpCML25 gene is cloned from chimonanthus praecox, the CDS region of the CpCML25 gene is 570 bp, and 189 amino acids are encoded. Under drought, salt, high temperature and low temperature stress and induction of hormones ABA and MeJA, CpCML25 is up-regulated to different degrees. And performing agrobacterium tumefaciens-mediated transformation on the overexpression vector to obtain the 35S:: CpCML25 / arabidopsis thaliana strain. Compared with a wild type, the overexpressed strain shows the phenomena that the flowering time is advanced, the plant height is lower, the rosette leaf length is smaller, the number of stem leaves and primary lateral branches is increased and the like. The overexpression CpCML25 improves the drought resistance of the arabidopsis thaliana, and reduces the salt tolerance and cold tolerance of the arabidopsis thaliana.

The invention discloses molecular markers for identifying five chimonanthus plants and application of the molecular markers in the technical field of molecular identification of plant species, specific molecular markers (cox2i691 and nad4i1399) are developed on the basis of variable regions between chimonanthus praecox and chimonanthus nitens mitochondrial genomes, and the five chimonanthus species are effectively distinguished. The components comprise chimonanthus praecox, chimonanthus nitens, chimonanthus salicifolius, Zhejiang chimonanthus praecox and chimonanthus nitens. According to the method, the chimonanthus species can be conveniently and accurately identified, important information is provided for chimonanthus evolutionary analysis and biochemical character formation mechanism analysis in the future, and chimonanthus species classification, mitochondrial genome evolutionary research and chimonanthus species derived product discrimination are also facilitated.

The present invention discloses the use of a Chimonanthus dahurica leaf extract in preparing a drug for treating allergic rhinitis, and relates to the field of traditional Chinese medicine. Based on an allergic rhinitis animal model, the present invention investigates the effects of Chimonanthus dahurica leaves on the behavior, nasal mucosal tissue morphology, and serum inflammatory factor levels of AR rats, and clarifies the pharmacodynamics of Chimonanthus dahurica leaves in treating AR. That is, Chimonanthus dahurica leaves can significantly reduce the levels of IgE, histamine, ICAM-1, and inflammatory factors in the serum of rats with allergic rhinitis, and can also significantly improve the nasal mucosal symptoms of rats with allergic rhinitis, among which the Chimonanthus dahurica leaf water extract has a better effect. At the same time, by preparing different active component groups of Chimonanthus dahurica leaves, the effective parts of Chimonanthus dahurica for treating allergic rhinitis are screened, among which the 70% ethanol elution group and the 50% ethanol elution group have the best effects. The present invention has prospects for developing and preparing drugs for treating allergic rhinitis.

The application discloses a kind of to improve agarwood tetrol content's odd Nan multi-generation grafting cultivation method, belong to plant breeding and cultivation technical field.The application aims at solving the existing odd Nan agarwood agarwood tetrol content is far lower than pharmacopoeia standard, cannot satisfy medicinal demand, and the existing grafting technology breeding goal is single, does not form the problem of directional breeding system.The application carries out grafting with agarwood tree species as rootstock and odd Nan branch as scion, after cultivation management, induces to be fragrant, with agarwood tetrol content as core index to screen excellent single plant, then with excellent plant branch as scion repeats multi-generation cycle cultivation, finally obtains the odd Nan strain with high agarwood tetrol content.The application can realize the stable promotion of agarwood tetrol content generation by generation, retain the core advantage of odd Nan rapid fragrant formation simultaneously, is suitable for the large-scale directional cultivation of odd Nan medicinal variety.

The invention relates to a chimonanthus praecox calmodulin protein CpCML45 and an application thereof. The CpCML45 gene is cloned from chimonanthus praecox, the maximum ORF frame of the CpCML45 gene is 627 bp, and 208 amino acids are encoded. According to the invention, a pCAMBIA-2301G-CpCML45 overexpression vector is constructed and is transferred into arabidopsis thaliana Col-0. Through overexpression of CpCML45, the leaf area of transgenic arabidopsis thaliana is significantly increased, the inflorescence is significantly increased, and the leaf area and the inflorescence are in direct proportion to the expression quantity. Through overexpression of CpCML45, drought tolerance and salt tolerance of transgenic arabidopsis thaliana are enhanced, but low temperature tolerance of the transgenic arabidopsis thaliana is reduced.