3 results about "Chimonanthus" patented technology

The invention relates to a chimonanthus praecox calmodulin protein CpCML25 and an application thereof. The CpCML25 gene is cloned from chimonanthus praecox, the CDS region of the CpCML25 gene is 570 bp, and 189 amino acids are encoded. Under drought, salt, high temperature and low temperature stress and induction of hormones ABA and MeJA, CpCML25 is up-regulated to different degrees. And performing agrobacterium tumefaciens-mediated transformation on the overexpression vector to obtain the 35S:: CpCML25 / arabidopsis thaliana strain. Compared with a wild type, the overexpressed strain shows the phenomena that the flowering time is advanced, the plant height is lower, the rosette leaf length is smaller, the number of stem leaves and primary lateral branches is increased and the like. The overexpression CpCML25 improves the drought resistance of the arabidopsis thaliana, and reduces the salt tolerance and cold tolerance of the arabidopsis thaliana.

The application discloses a kind of to improve agarwood tetrol content's odd Nan multi-generation grafting cultivation method, belong to plant breeding and cultivation technical field.The application aims at solving the existing odd Nan agarwood agarwood tetrol content is far lower than pharmacopoeia standard, cannot satisfy medicinal demand, and the existing grafting technology breeding goal is single, does not form the problem of directional breeding system.The application carries out grafting with agarwood tree species as rootstock and odd Nan branch as scion, after cultivation management, induces to be fragrant, with agarwood tetrol content as core index to screen excellent single plant, then with excellent plant branch as scion repeats multi-generation cycle cultivation, finally obtains the odd Nan strain with high agarwood tetrol content.The application can realize the stable promotion of agarwood tetrol content generation by generation, retain the core advantage of odd Nan rapid fragrant formation simultaneously, is suitable for the large-scale directional cultivation of odd Nan medicinal variety.

The invention relates to a chimonanthus praecox calmodulin protein CpCML45 and an application thereof. The CpCML45 gene is cloned from chimonanthus praecox, the maximum ORF frame of the CpCML45 gene is 627 bp, and 208 amino acids are encoded. According to the invention, a pCAMBIA-2301G-CpCML45 overexpression vector is constructed and is transferred into arabidopsis thaliana Col-0. Through overexpression of CpCML45, the leaf area of transgenic arabidopsis thaliana is significantly increased, the inflorescence is significantly increased, and the leaf area and the inflorescence are in direct proportion to the expression quantity. Through overexpression of CpCML45, drought tolerance and salt tolerance of transgenic arabidopsis thaliana are enhanced, but low temperature tolerance of the transgenic arabidopsis thaliana is reduced.