96 results about "Chimonanthus praecox" patented technology

The invention belongs to the technical field of planting and particularly relates to a chimonanthus praecox cutting propagation method. The chimonanthus praecox cutting propagation method includes the following steps that firstly, cutting is conducted at the beginning of June, and a cutting medium is prepared 2 days before cutting and disinfected; secondly, a chimonanthus praecox tender branch is obtained, the upper portion of the branch is cut into a shoot for cutting, a lower notch of the shoot for cutting is in a horseshoe shape, and the shoot for cutting is steeped and disinfected after cutting is conducted; thirdly, 1-2 cm of the lower portion of the shoot for cutting obtained in the second step dips in an indolebutyric acid solution of 1500-2500 mg / Kg for 8-12 seconds and then is cut into the medium in the step A to be fixed, wherein the indolebutyric acid solution is prepared by ethyl alcohol with the volume percentage of 50 percent; fourthly, disinfecting is conducted each week, the relative humidity on the periphery of the cut shoot for cutting is controlled to be 85-95 percent in a spraying mode, the environment temperature does not exceed 40 DEG C until a lateral root grows out, and a cutting seedling is obtained and transplanted. According to the chimonanthus praecox cutting propagation method, the survival rate is high and can reach 85 percent to the maximum, the ornamental value and rapid propagation of chimonanthus praecox varieties can be easily kept, segregation of characters cannot happen, and the method has the quite good industry application prospect.

The invention relates to the field of traditional Chinese medicine preparation and relates to a preparation method of a chimonanthus inhibited acetylcholinesterase active part and application thereof. The preparation method comprises the following steps of: ethanol lixiviation, low-polarity solvent extraction, acid-solution and alkali-isolation chloroform extraction or alkali-solution and acid-isolation, centrifugation and ethyl acetate extraction. The extracted active part includes three categories of substances, namely alkaloids, flavonoids and coumarins respectively. The active part has relatively strong inhabitation effect on the activity of acetylcholinesterase and has a wide application range in preparing medicaments for preventing and treating related symptoms or diseases caused by acetylcholine deletion, particularly the medicaments for preventing and treating senile dementia.

The invention discloses an efficient in-vitro regeneration method for chimonanthus praecox plants. The method includes the steps: clipping mature chimonanthus praecox seed shells, disinfecting surfaces, inoculating a seed germination culture medium with seeds for rapid germination, and performing light culture to obtain chimonanthus praecox seed seedlings; inoculating the culture medium with stem segments of the chimonanthus praecox seed seedlings serving as explants under an aseptic condition, and sequentially performing germination induction, multiplication, extension and rooting culture on stem segments with axillary buds; domesticating and transplanting obtained aseptic chimonanthus praecox seedlings to finally obtain complete chimonanthus praecox regeneration plants. Different growth regulating agents are added into the culture medium. The efficient in-vitro regeneration method for the chimonanthus praecox plants has the advantages of short breeding cycle, high breeding coefficient and good repeatability.

The invention belongs to the field of medicines and health products, and concretely relates to an application of Chimonanthus salicifolius total alkaloid extract and monomer compounds thereof in the preparation of medicines or health products for treating gastrointestinal tumor diseases. The Chimonanthus salicifolius total alkaloid extract and chimonanthine monomer compounds (1-4) are extracted and prepared from a Calycanthaceae Chimonanthus plant Chimonanthus salicifolius, and experiments prove that the Chimonanthus salicifolius total alkaloid extract can substantially inhibit proliferation of stomach cancer SGC-7901, colorectal carcinoma SW1116 and colorectal carcinoma HCT116 cells and induce cancer cell apoptosis, and the alkaloid monomer compounds have different inhibition effects on the stomach cancer SGC-7901, colorectal carcinoma SW1116 and colorectal carcinoma HCT116 cells. The Chimonanthus salicifolius extract and the monomer compounds thereof can be applied individually or together or be combined with a suitable excipient, can be processed through a routine method to prepare an oral or non-oral dosage form used for treatment or assisted treatment of gastrointestinal tumor diseases, and is especially suitable for being used to prepare medicines or health products for treating stomach cancer and colorectal carcinoma.

The invention relates to a landscape formation method of an all-season-fragrant plant community hierarchical structure, belonging to the technical field of garden engineering. The plant community combination established by the method is formed by compositely planting various fragrance-releasing arbors, shrubs and herbaceous plants in different layers. Michelia alba, Cinnamomum camphora, Ginkgo biloba, Cedrus deodara, Pittosporum tobira, Gardenia jasminoides, Osmanthus fragrans, Chimonanthus praecox and many other plants capable of giving out the respective fragrance in different seasons are adopted, and are reasonably matched and planted in the landscape green central parting region according to the fragrance release seasons and months, positions, terrains, fragrance release thickness, air humidity, hue of floral leaves and the like, thereby providing a large-area all-season-fragrant environment for local tourists, residents, patients and the like, and benefiting the recovery and life prolonging. The formation method takes the seasonal aspect changes of plants into consideration, enriches the landscape configuration of all seasons, creates an all-season-fragrant garden environment, ensures the continuity of fragrant landscape, and has the functions of cleaning air, repelling mosquitoes, preventing worms, relieving the pressure, inhibiting the pathogenic bacteria and the like.

The invention discloses a supercritical CO2 extraction method for chimonanthus praecox essential oil. The supercritical CO2 extraction method comprises the following specific steps: placing pretreated chimonanthus praecox containing 70% of water into an extraction kettle for extracting; controlling pressure, temperature, time and CO2 flow; then, separating an extracting solution in a two-stage analytic kettle to obtain crude chimonanthus praecox essential oil; finally, centrifuging to obtain pure essential oil. The problems that peculiar cooking smell is generated in extracting the chimonanthus praecox essential oil by using the traditional water vapor distillation method, an organic solvent is easy to remain in a product if an organic solvent extraction method is used and potential safety hazards exist in the production process are solved. According to the invention, the natural fragrance of chimonanthus praecox is remained in the essential oil, and the essential oil can be used as a high-grade essence with high added value; in addition, no organic matters are remained in the essential oil, and no potential safety hazards exist in the production process.

The invention provides a method for low-temperature induction control over the chimonanthus praecox flowering phase. The method for low-temperature induction control over the chimonanthus praecox flowering phase comprises the steps that chimonanthus praecox is placed under the temperature of 12 DEG C or below for low-temperature treatment, and when the cold amount reaches 570 CU, chimonanthus praecox buds can normal expand and bloom. According to the 'Utah model' and '7.2 DEG C model', the chimonanthus praecox flowering stage morphologic data can be observed and counted, meanwhile, the 'suxin'and 'qingkou' chimonanthus praecox variety blooming cooling capacities are preliminarily researched, and results show that the 'suxin' and 'qingkou' chimonanthus praecox variety blooming cooling capacities are 558 CU and 570 CU respectively. On the basis, 12-year-old robust potted 'suxin' chimonanthus praecox seedlings are subjected to different-low-temperature artificial induction treatment, andbuds treated under the temperatures of 4,8,12 DEG C can normally expand and bloom, wherein expand and blooming can be achieved when the cooling capacity under the temperature of 12 DEG C can reach 570 CU. the method can effectively control the chimonanthus praecox flowering stages, and the chimonanthus praecox can bloom ahead of the schedule or be postponed.

The invention relates to a medical pillow with an insomnia prevention and treatment function. The medical pillow comprises a pillow interior. Medicinal powder filled in the pillow interior includes 50 grams of cough chrysanthemum, 85 grams of henon bamboo juvenile leaf, 30 grams of slender falsenettle herb, 50 grams of chimonanthus praecox link, 35 grams of all-grass of bulley herminium, 50 grams of albizia julibrissin, 15 grams of lotus, 30 grams of jujube leaves, 35 grams of rhodiola rosea, 15 grams of cinnamomum subavenium, 20 grams of kuanyin tea, 35 grams of chrysanthemum, 20 grams of perilla nankinensis decue, 30 grams of leonurus heterophyllus, and 10 grams of mioga ginger.

The invention relates to a cutting cultivation method for chimonanthus praecox. The method comprises the steps of: firstly selecting cuttings; soaking the cuttings by a potassium permanganate solution and indoleacetic acid in sequence; then preparing a slotting machine; inserting the cuttings into the slotting machine according to certain plant spacing; and carrying out final-period management so as to ensure that young leaves do not fall off. When the cuttings germinate the second stubbles of leaves, the cutting cultivation method is completed. According to the cutting cultivation method provided by the invention, the slotting machine provided by the invention uses river sand, perlite, glass beads and wet sand, and organic fertilizers are added into the slotting machine. Then a leaf fertilizer is sprayed periodically. Through a series of mutually matched steps, the cuttings take root in about 4 months, and the survival rate is improved by 10% compared to that of a conventional method. Moreover, the survival rate and the growing trend after transplanting are superior to those in the prior art.

The invention relates to the field of tea production, in particular to a processing method for making gunpowder tea from chimonanthus praecox. The method comprises the following steps of chimonanthus praecox leaf picking, wherein a bud and a bud and two leaves are picked, the length is controlled to be within 6 cm, buds and leaves are uniform, no old leaf or stalk stem or impurity exists, air-permeable bamboo baskets and baskets are adopted as containing tools, and the leaves are not compressed; spreading, wherein chimonanthus praecox leaves are spread on a bamboo tray thinly, the thickness ranges from 5 cm to 8 cm, the leaves are 4-6 kg per square meter, and the spreading time is 2-3 h; fixation, wherein chimonanthus praecox leaves obtained after spreading are put into a fixation machine, 2-3 kg of leaves are put into each pot, the temperature of each pot is 280 DEG C, the method of covering first and then throwing is adopted, the temperature for fixation is 80-90 DEG C, and the fixation time ranges from 2.5 min to 3 min; rolling, wherein the chimonanthus praecox leaves obtained after fixation are rolled through a rolling machine, the leaves are loaded to the position 4-6 cm away from a barrel opening, the light-heavy-light principle is adopted in the rolling process, and the rolling time ranges from 30 min to 40 min, the rolled twig rate reaches 80%-90%, and the cell broken rate is 45%-60%. The processing method is simple, easy to implement, the product quality can be guaranteed, the high economic benefit is achieved, and popularization is easy.

The invention belongs to the technical field of molecular biology, and particularly relates to a chimonanthus praecox CpSRG1 gene, a promoter and application of the Chimonanthus praecox CpSRG1 gene. The invention provides a chimonanthus praecox CpSRG1 protein, a gene for coding the protein, cloning of the promoter and application of the chimonanthus praecox CpSRG1 protein. The chimonanthus praecox CpSRG1 gene is obtained through cloning for the first time, the expression characteristic of the CpSRG1 gene is detected through a real-time fluorescent quantitative PCR technology, and a function of the promoter of the gene is verified in a plant (arabidopsis thaliana) through a transgenic technology. Reference and basis are provided for research on senescence, stress response (hormone) and regulation mechanism of chimonanthus praecox and leaves, and possibility is provided for further improvement of ornamental plants, such as prolonging of the flowering phase of chimonanthus praecox.

The invention relates to the technical field of washing products, in particular to Chimonanthus praecox petal facial cleansing soap and a production method thereof; the Chimonanthus praecox petal facial cleansing soap is produced from, by weight, 10-20 parts of stearic acid, 5-10 parts of propylene glycol, 10-20 parts of sucrose, 5-10 parts of lauric acid, 8-15 parts of sorbitol, 10-15 parts of water, 3-6 parts of sodium lauryl sulfate, 4-8 parts of sodium laureth sulfate, 1-2 parts of sodium hydroxide, 3-8 parts of ethanol, 2-5 parts of plum extract, 0.1-0.5 part of Chimonanthus praecox petal, 0.3-1 part of algal extract, 1-3 parts of wheat germ oil, 1-2 parts of camellia seed oil, 0.2-1 part of Simmondsia chinensis seed oil, 0.1-2 parts of olive oil, 0.1-0.8 part of avocado oil, 0.3-1 part of trehalose, 0.05-0.1 part of allantoin, and 0.05-0.5 part of an essence. The Chimonanthus praecox petal facial cleansing soap has moisturizing effect, wrinkle removal effect, skin-moistening effect, skin smoothness maintaining effect, anti-aging effect, antibacterial effect, antioxidant effect, anti-allergy, nerve-soothing effect and other effects.

The invention belongs to the technical field of genetic engineering, and particularly relates to the cloning of a Calycanthus praecox WRKY transcription factor gene CpWRKY71 and its promoter and an application thereof. The object of the present invention is to provide a new option for the study of adversity stress of Calycanthus praecox. The present invention provides the Calycanthus praecox WRKYtranscription factor gene CpWRKY71, and its encoded protein has an amino acid sequence shown as SEQ ID No.3. The Calycanthus praecox CpWRKY71 gene is cloned for the first time, and the expression characteristics of CpWRKY71 gene are detected by a real-time fluorescent quantitative PCR technology, and the function of the gene promoter is verified in Arabidopis thaliana by a transgenic technology.

The invention belongs to a preparation method of tobacco flavor, and specifically relates to acid flavor type tobacco Chimonanthus Praecox flavor and its preparation method. The flavor provided by the invention is prepared by steps of: adding Chimonanthus Praecox into water for reflux extraction and fermenting the extract product by the use of Clostridium butyricum CB-2. With Chimonanthus Praecox being used as a raw material, the tobacco Chimonanthus Praecox flavor with acid flavor and traditional Chinese medicine fragrance is produced by a microbial fermentation technology. With the addition of the flavor, tobacco has rich smoke fragrance, the smoke gas is soft, and the tobacco stimulation is reduced.

The invention relates to a health care medicated soap, which is prepared from the following raw materials by weight percentage: 40% of a soap base, 10% of glycerin, 5% of chimonanthus praecox root, 10% of Cerastium viscosum L., 10% of Cynanchum atratum root, 5% of Toona Sinensis Roem tender leaf, 5% of Cryptotaenia japonica, 10% of Nepeta Cataria root, and 5% of Youngia japonica root. The health care medicated soap provided by the invention has the efficacy of dispelling wind, diminishing inflammation, clearing heat, removing toxin, activating blood circulation and dispersing swelling, and has supportive therapeutic efficacy on skin itching.

The invention provides a plant foliar bactericide with the antifreezing function, relating to the technical field of pesticides. The plant foliar bactericide is prepared from a bactericidal composition and an antifreezing composition in a ratio of 1:2, wherein the bactericidal composition comprises the following components in parts by weight: 5-15 parts of garlic powder, 10-30 parts of lime powder, 0.1-1 part of alkaloids from tea, 0.1-2 parts of capsaicin, 5-15 parts of calcium hypochlorite and 20-55 parts of hydrogen peroxide; the antifreezing composition comprises the following components in parts by weight: 5 parts of pine needles, 5 parts of dracaena sanderiana, 5 parts of cinnamomum camphora, 15 parts of chimonanthus praecox, 15 parts of holly, 1 part of wheat seedling, 3 parts of oxyspora paniculata, 0.05 part of antifreezing fluid for automobiles, 0.01 part of castor oil and 0.1 part of preservative. The plant foliar bactericide can be used for perennial plants in most parts of China, such as all the vegetables, fruit trees, flowers and plants and bonsai, can enhance the water retention and cold resistance of the plants, has relief and recovery effects on the plants suffering moderate and mild freezing injuries, adds chlorophyll, increases the yields and quality of crops and lengthens the green period.

The invention provides a method of growing seedlings of Chimonanthus praecox by layering. The method comprises selecting a triennial Chimonanthus praecox tree as a mother tree, and carrying out layering when root shoots grow to 0.4-0.6 cm in thickness and greater than 50 cm in length; before layering, carrying bud notching 0.2-0.4 cm away from the bottom of each bud at intervals of 14-16 cm; laying the shoots at the above convenience, and compressing and burying the shoots tightly with soil; after rooting occurs in September, dividing plants, and transplanting. The method provided herein is simple to perform, has few time limits and provides high survival rate.

The invention provides a chimonanthus praecox CpVIN3 gene and application of the chimonanthus praecox CpVIN3 gene in flowering phase regulation. A gene sequence of the chimonanthus praecox CpVIN3 provided by the invention is shown as SEQ ID No.1. The chimonanthus praecox CpVIN3 gene is obtained through cloning for the first time, and the function of the gene is verified in plant arabidopsis thaliana through a transgenic technology. The method comprises the following steps: transferring a CpVIN3 gene into arabidopsis thaliana; the phenotype of the gene is observed to preliminarily verify the function of the gene; compared with the phenotype of transgenic arabidopsis thaliana and the phenotype of wild arabidopsis thaliana, the flowering time of the transgenic arabidopsis thaliana is earlierthan that of the wild arabidopsis thaliana under the condition of 4 DEG C vernalization treatment, and the number of rosette leaves is reduced compared with that of the wild arabidopsis thaliana, which indicates that the gene regulates plant nutrition growth to reproductive growth in the vernalization process.

The invention relates to a medicinal and edible plant chimonanthus grammatus essential oil, and a preparation method and application thereof. The chimonanthus grammatus essential oil is extracted by a vapor distillation method; 53 components are detected by gas-phase chromatogram-mass spectrum combined technology; the identified components occupy 99.99 percent of total peak area of a chromatogram of volatile oil; and the main components of the essential oil are (E)-3-(4,8-dimethyl-3,7-nonene)-furan, acetyl borneol ester, 6,6-dimethyl-3-methylene-bicyclo[3.1.1] heptane, tetrahydro solasodine, ledene alcohol, 1,5-dimethyl-1-ethenyl-4-hexenyl butyrate and the like which account for 49.87 percent of the total components of the volatile oil. The essential oil has higher antibacterial activity, wider antibacterial spectrum and high anti-oxidation activity; and can be used as a natural antibacterial and antioxidant active ingredient for preparing cosmetic additives, food additives, medicinal products, health-care products and the like.

The invention provides a CpSOC1 gene of chimonanthus praecox, protein encoded by the CpSOC1 gene and application of the gene in regulation of a flowering stage. The chimonanthus praecox CpSOC1 protein provided by the invention is 1) a protein consisting of amino acids shown as SEQ ID NO. 2; or 2) a protein derived from the protein 1) by substitution, deletion or addition of one or more amino acids in an amino acid sequence shown as SEQ ID NO. 1 and having the same activity. The invention also provides a gene for encoding the protein. According to the invention, cloning is carried out to obtain CpSOC1 gene of chimonanthus praecox for the first time, and the function of the gene is verified in a plant (arabidopsis thaliana) by a transgenic technology. Results show that the flowering stage of transgenic arabidopsis thaliana is earlier than that of a control group, and the survival time of calyxes of transgenic Arabidopsis thaliana having a high expression level and a moderate expression level is significantly longer than that of the control group, thereby providing the possibility for the regulation of the flowering stage and the increase of the ornamental value of the plant.

The invention discloses toothpaste containing oral probiotics and a preparation method thereof. The toothpaste comprises the following raw materials in parts by weight: 40-60 parts of Borojo powder, 30-60 parts of lithocarpus litseifolius, 10-20 parts of probiotics, 1-5 parts of chimonanthus salicifolius, 1-5 parts of mannose oligomer, 1-5 parts of lindera aggregata leaves and 1-5 parts of colostrum basic protein. According to the invention, the Borojo powder and the lithocarpus litseifolius which have anti-oxidation effects are adopted, so that the effect of treating oral ulcer is achieved; the dosage of the probiotics can be reduced; the mannose oligomer can promote the growth of the probiotics, so that the probiotics play a synergistic role; and meanwhile, the contained chimonanthus salicifolius, rice bran fatty alkanol, lindera aggregata leaves and colostrum basic protein all have the effect of treating the oral ulcer, so that the oral ulcer is effectively prevented and improved.

The invention belongs to the field of daily necessities, and particularly relates to the application of a chimonanthus praecox seed oil-containing composition to a shampoo and a shower gel. The invention provides the chimonanthus praecox seed oil-containing composition, and the composition comprises chimonanthus praecox seed oil, a plum blossom extract, plum blossom water, a surfactant, a wettingagent, a moisturizer, a conditioner, a thickener and a preservative. The composition can be applied to preparation of the shampoo or the shower gel, and the chimonanthus praecox seed oil is rich in unsaturated fatty acids and rich in nutrients and has the efficacies of moisturizing and soothing skin. When the composition is applied to the shampoo, the chimonanthus praecox seed oil can smoothen hair and moisturize scalp; when the composition is applied to the shower gel, the antioxidant function of the chimonanthus praecox seed oil prevents chimonanthus praecox petals in the shower gel from fading and can moisturize the skin. The shampoo and the shower gel prepared from the composition have a good using feel, are rich in the chimonanthus praecox seed oil and the plum blossom extract while the hair or the skin is cleansed, and can directly enable the nutrients to penetrate into the skin so as to quickly moisturize the skin.

The invention relates to the field of plant molecular biology, in particular to a C. praecox CpUFO gene as well as coded protein and an application thereof. A cDNA sequence of the C. praecox CpUFO is obtained by gene cloning, the whole length of the cDNA sequence of the gene is 1753 bp, and the gene contains 1212 bp complete ORF (open reading frame) and codes 403 amino acids. After the gene is transformed into arabidopsis thaliana, phenotype observation finds that the bud emergence time, tillering time, first flower blooming time and first pod emergence time of a transgenic line are earlier than a WT plant; the rosette leaf number and total leaf number are fewer than the WT plant, and the blooming time is early. Flower organ observation finds that arabidopsis thaliana pistils genetically modified with the CpUFO gene at the same blooming stage are longer than wild type arabidopsis thaliana, which indicates that the C. praecox CpUFO gene can promote plants to bloom and has influence on flower organs of plants.

The invention provides a Chimonanthus praecox CpFT gene and application thereof in florescence regulation. A gene sequence of the Chimonanthus praecox CpFT is represented as SEQ ID No.1. By the adoption of the Chimonanthus praecox CpFT gene and the application thereof in the florescence regulation, the Chimonanthus praecox CpFT gene is obtained by cloning for a first time, and the function of thegene is verified in plant Arabidopsis thaliana by a transgenic technology. The results show that the higher the expression quantity of the Chimonanthus praecox CpFT gene, the smaller the number of rosette leaves in transgenic Arabidopsis thaliana, and the shorter the time of sprouting, the flowering time of a first flower and the forming time of a first pod. As the time of plant transformation from vegetative growth to reproductive growth is advanced, the vegetative growth time is shortened, the number of the rosette leaves is reduced, and the flowering time is advanced. The results show thatthe Chimonanthus praecox CpFT gene can promote the flowering of plants and inhibit the vegetative growth of the plants at the same time, and the gene plays an important role in a process of floral bulging and flowering caused by breaking dormancy of flower buds of the Chimonanthus praecox at low temperature.

The invention belongs to the technical field of plant gene functions, and particularly relates to a chimonanthus praecox GDSL lipase gene CpGLIP1 and application thereof. The invention provides a chimonanthus praecox CpGLIP1 gene. An amino acid sequence of a coding protein of the chimonanthus praecox CpGLIP1 gene is shown as SEQ ID No. 1. The chimonanthus praecox CpGLIP1 gene is obtained through cloning for the first time, expression characteristic analysis and prokaryotic expression are carried out, and the function of the gene is verified in a plant (arabidopsis thaliana) through a transgenic technology. The overexpression of the chimonanthus praecox CpGLIP1 gene in the arabidopsis thaliana can improve the drought tolerance and cold resistance of the arabidopsis thaliana, and the result provides a basis for the research on the abiotic stress response of the chimonanthus praecox and provides a reference for improving the abiotic stress tolerance of ornamental plants.

The invention belongs to the technical field of planting and particularly relates to a chimonanthus praecox cutting propagation method. The chimonanthus praecox cutting propagation method includes the following steps that firstly, cutting is conducted at the beginning of June, and a cutting medium is prepared 2 days before cutting and disinfected; secondly, a chimonanthus praecox tender branch is obtained, the upper portion of the branch is cut into a shoot for cutting, a lower notch of the shoot for cutting is in a horseshoe shape, and the shoot for cutting is steeped and disinfected after cutting is conducted; thirdly, 1-2 cm of the lower portion of the shoot for cutting obtained in the second step dips in an indolebutyric acid solution of 1500-2500 mg / Kg for 8-12 seconds and then is cut into the medium in the step A to be fixed, wherein the indolebutyric acid solution is prepared by ethyl alcohol with the volume percentage of 50 percent; fourthly, disinfecting is conducted each week, the relative humidity on the periphery of the cut shoot for cutting is controlled to be 85-95 percent in a spraying mode, the environment temperature does not exceed 40 DEG C until a lateral root grows out, and a cutting seedling is obtained and transplanted. According to the chimonanthus praecox cutting propagation method, the survival rate is high and can reach 85 percent to the maximum, the ornamental value and rapid propagation of chimonanthus praecox varieties can be easily kept, segregation of characters cannot happen, and the method has the quite good industry application prospect.

The invention discloses a porobiotic composition for treating oral ulcer as well as a preparation method and application thereof. The porobiotic composition comprises the following raw materials in parts by weight: 40-60 parts of borojoa sorbilis cuter powder, 30-60 parts of lithocarpus litseifolius, 10-20 parts of probiotics, 1-5 parts of chimonanthus salicifolius, 1-5 parts of mannose oligomer, 1-5 parts of combined spicebush leaves, and 1-5 parts of colostrum basic protein. In virtue of the borojoa sorbilis cuter powder and the lithocarpus litseifolius adopted, the porobiotic composition is endowed with the effect of treating oral ulcer. Moreover, the dosage of the probiotics can be reduced; and the growth of the probiotics can be promoted by the mannose oligomer, so that the probiotics can play a synergistic role. In addition, the chimonanthus salicifolius, rice bran fatty alkanol, the combined spicebush leaves and the colostrum basic protein all have the effect of treating oral ulcer, so that oral ulcer can be effectively prevented and improved.

The invention discloses chimonanthus nitens-containing tea and a preparation method thereof. According to the preparation method, adopted tea leaves are composed of chimonanthus nitens leaves and teatree leaves at a weight ratio of 1:4-5. The preparation method comprises the steps of picking, pretreatment, primary enzyme deactivation, secondary enzyme deactivation, rolling and drying. The chimonanthus nitens leaves and the tea tree leaves are reasonably proportioned by weight; according to the present invention, the tea leaves are prepared by using the scientific and reasonable preparation process, the sensory evaluation effect of the obtained tea is good, more antioxidant substances can be retained, especially the classified enzyme deactivating manner is used, and an enzymatic hydrolysate is sprayed during the secondary enzyme deactivation, such that the sensory score of the tea and the content of total polyphenols and flavonoids can be substantially improved, and the quality of thetea is substantially improved.

The invention discloses a novel alkaloid compound chimonin-2, 3-diketone separated from dry leaves of chimonanthus nitens, and the novel alkaloid compound chimonin-2, 3-diketone has the following structure: in vitro test research proves that the compound can inhibit replication of influenza A virus H1N1 and has good anti-influenza A virus H1N1 activity. The compound can be further researched and developed into anti-influenza virus drugs.