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Efficient in-vitro regeneration method for chimonanthus praecox plants

An in vitro regeneration, wax plum technology, applied in plant regeneration, botanical equipment and methods, horticultural methods, etc., can solve the problems of limiting the application of plant tissue culture technology, poor repeatability, low reproduction coefficient, etc., and achieve fast regeneration. , the effect of high regeneration efficiency and high reproduction coefficient

Active Publication Date: 2017-08-04
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few studies on the propagation of Wintersweet by using tissue culture technology. Among them, the research on the in vitro regeneration of Wintersweet with field stems and cotyledons as explants has been reported, but it is still in the primary research stage. Problems such as long cycle, low reproduction coefficient, poor repeatability and serious browning severely limit the application of plant tissue culture technology in wintersweet breeding

Method used

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  • Efficient in-vitro regeneration method for chimonanthus praecox plants
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  • Efficient in-vitro regeneration method for chimonanthus praecox plants

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Embodiment 1

[0026] A kind of method for the high-efficiency in vitro regeneration of Wintersweet plant of the present embodiment, comprises the following steps:

[0027] (1) surface disinfection of wax plum seeds:

[0028] Take the mature wintersweet seeds and cut off the outer shell, rinse the undamaged seeds with sterile water three times in a sterile operating table, disinfect with 70% absolute ethanol for 15 seconds, and then use 18% hydrogen peroxide The solution was disinfected for 2 minutes, ultrasonically treated for 4 minutes, and finally rinsed with sterile water 4 times, and kept shaking during the disinfection period;

[0029] (2) Germination and cultivation of wax plum seeds:

[0030] Inoculate the sterilized seeds in step (1) into 1 / 2DKW medium with 0.02mg / L GA3, 25g / L sucrose and 6.5g / L agar added, at a temperature of 25±2°C and a light condition of 2000lx Germination culture was carried out in a constant temperature culture room in the same country. After culturing for 7...

Embodiment 2

[0042] A kind of method for the high-efficiency in vitro regeneration of Wintersweet plant of the present embodiment, comprises the following steps:

[0043] (1) surface disinfection of wax plum seeds:

[0044]Take the mature wintersweet seeds and cut off the shell, rinse the undamaged seeds with sterile water 4 times in the aseptic operating table, disinfect with 75% absolute ethanol for 30 seconds, and then use 20% hydrogen peroxide The solution was disinfected for 5 minutes, ultrasonically treated for 6 minutes, and finally rinsed with sterile water for 5 times, and kept shaking during the disinfection period;

[0045] (2) Germination and cultivation of wax plum seeds:

[0046] Inoculate the sterilized seeds in step (1) into 1 / 2DKW medium with 0.05mg / L GA3, 30g / L sucrose and 7.0g / L agar added, at a temperature of 25±2°C and a light condition of 2200lx The constant temperature culture chamber of the present invention carries out germination culture, and after cultivating 8...

Embodiment 3

[0058] A kind of method for the high-efficiency in vitro regeneration of Wintersweet plant of the present embodiment, comprises the following steps:

[0059] (1) surface disinfection of wax plum seeds:

[0060] Take the mature wintersweet seeds and cut off the shell, rinse the undamaged seeds with sterile water 5 times in a sterile operating table, disinfect with 75% absolute ethanol for 45 seconds, and then use 22% hydrogen peroxide The solution was sterilized for 10 minutes, ultrasonically treated for 10 minutes, and finally rinsed 6 times with sterile water, and kept shaking during the disinfection period;

[0061] (2) Germination and cultivation of wax plum seeds:

[0062] Inoculate the sterilized seeds in step (1) into 1 / 2DKW medium with 0.1mg / L GA3, 35g / L sucrose and 7.5g / L agar, at a temperature of 25±2°C and a light condition of 2500lx Germination culture was carried out in a constant temperature culture room in the same country, and after culturing for 10 days, seed...

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Abstract

The invention discloses an efficient in-vitro regeneration method for chimonanthus praecox plants. The method includes the steps: clipping mature chimonanthus praecox seed shells, disinfecting surfaces, inoculating a seed germination culture medium with seeds for rapid germination, and performing light culture to obtain chimonanthus praecox seed seedlings; inoculating the culture medium with stem segments of the chimonanthus praecox seed seedlings serving as explants under an aseptic condition, and sequentially performing germination induction, multiplication, extension and rooting culture on stem segments with axillary buds; domesticating and transplanting obtained aseptic chimonanthus praecox seedlings to finally obtain complete chimonanthus praecox regeneration plants. Different growth regulating agents are added into the culture medium. The efficient in-vitro regeneration method for the chimonanthus praecox plants has the advantages of short breeding cycle, high breeding coefficient and good repeatability.

Description

technical field [0001] The invention relates to the field of rapid propagation of wintersweet plants, in particular to a method for efficient in vitro regeneration of wintersweet plants. Background technique [0002] Wintersweet (Chimonanthus praecox (Linn.) Link), also known as Chimonanthus praecox (Linn.) Link, is a deciduous bush of Chimonanthus praecox (Linn.) Link. It is a multifunctional tree species with ornamental, environmental protection and medicinal values. Wintersweet flowers bloom in the cold winter, the flowers are yellow as wax, fragrant, and have a long flowering period. They have ornamental value in color, fragrance, shape and posture. Plum is also a precious winter and early spring cut flower species with a natural vase life of more than three weeks. Wintersweet has a pleasant fragrance, and the ingredients of the extracted essence are more abundant than rose oil and jasmine essence. In the international market, the price of wax plum fragrance oil is much...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 侯金艳吴丽芳
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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