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A kind of method of seashore hibiscus tissue culture regeneration

A technique of hibiscus and tissue culture, applied in the biological field, achieves the effect of not being restricted by seasons, rich in materials, and convenient to obtain materials

Active Publication Date: 2019-05-03
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the research on the tissue culture of seaside hibiscus is still blank

Method used

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  • A kind of method of seashore hibiscus tissue culture regeneration
  • A kind of method of seashore hibiscus tissue culture regeneration
  • A kind of method of seashore hibiscus tissue culture regeneration

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The method for tissue culture and regeneration of seashore hibiscus in this embodiment is carried out under aseptic conditions and includes the following steps:

[0029] (1) Select perennial seaside hibiscus plant lines with excellent agronomic traits (one or more of high-yield, high-quality, and good stress resistance) from the field, and cut the unlignified branches of the current year as the source of explants ;

[0030] (2) After removing the leaves from the branches, cut them into 5cm-sized stems and perform surface disinfection; after surface disinfection, cut the necrotic parts at both ends of the stems, and then cut the stems into at least one axillary bud with a length of 0.7 Cut into sections with cm in size and set aside; among them, the method of surface disinfection is: rinse the original branches of seaside hibiscus with running water for 20 minutes, wipe with 75% absolute ethanol, and disinfect with 75% absolute ethanol in a sterile operating table for 30 seco...

Embodiment 2

[0036] The method for tissue culture and regeneration of seashore hibiscus in this embodiment is carried out under aseptic conditions and includes the following steps:

[0037] (1) Select perennial seaside hibiscus plant lines with excellent agronomic traits (one or more of high-yield, high-quality, and good stress resistance) from the field, and cut the unlignified branches of the current year as the source of explants ;

[0038] (2) After removing the leaves from the branches, cut them into 5cm-sized stems for surface disinfection; after surface disinfection, cut the stems into 0.5cm-long sections with at least one axillary bud for use; among them, the surface The method of disinfection is as follows: Rinse the fresh branches of seaside hibiscus with running water for 10 minutes, wipe with 74% absolute ethanol, disinfect with 74% absolute ethanol in a sterile operating table for 15 seconds; then disinfect with 10% hydrogen peroxide solution After 2 minutes, disinfect with 0.05% ...

Embodiment 3

[0044] The method for tissue culture and regeneration of seashore hibiscus in this embodiment is carried out under aseptic conditions and includes the following steps:

[0045] (1) Select perennial seaside hibiscus plant lines with excellent agronomic traits (one or more of high-yield, high-quality, and good stress resistance) from the field, and cut the unlignified branches of the current year as the source of explants ;

[0046] (2) After removing the leaves from the branches, cut them into 5cm-sized stems for surface disinfection; after surface disinfection, cut the stems into 1.0cm-long sections with at least one axillary bud for use; among them, the surface The method of disinfection is as follows: Rinse the fresh branches of seaside hibiscus with running water for 30 minutes, wipe with 76% absolute ethanol, disinfect with 76% absolute ethanol in a sterile operating table for 50s; then disinfect with 20% hydrogen peroxide solution After 8 minutes, disinfect with 0.15% mercury...

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Abstract

The invention discloses a tissue culture regeneration method of Hibiscus hamabo, comprising the steps of (1) using an underyearling non-lignified shoot of Hibiscus hamabo as an explant source, sterilizing the surface, and cutting the short into segments of suitable size; (2) inoculating the segments to culture media which plant growth regulators of different types and concentrations are added to, and performing sprouting of axillary buds, proliferating, elongating of adventitious buds, and rooting induced culture under suitable lighting and temperature; (3) acquiring regenerated plants of Hibiscus hamabo with strong roots. The method of the invention has the advantages that regeneration efficiency is high, proliferation coefficient is large, good character of female plants can be maintained, basis is laid for rapid large-scale reproduction of good strains of Hibiscus hamabo, and important technical support is also provided for genetically modifying Hibiscus hamabo in later stage by molecular biological means.

Description

Technical field [0001] The present invention relates to the field of biotechnology, in particular to a method for tissue culture and regeneration of seashore hibiscus. Background technique [0002] Seaside hibiscus (Hibiscus hamabo Sieb.et Zucc.) is a small deciduous tree of the genus Malvaceae Hibiscus. It is native to the coastal areas such as Zhoushan, Ningbo, Zhejiang. The rear leaves turn red, the seasonal changes are obvious, and the branches are tough and resistant to pruning. It is a very precious flower and leaf landscaping tree. In addition, the seaside hibiscus also has amazing resistance to stress, not only tolerant to salt and seawater Invasion, it also has strong drought tolerance, barren tolerance and wind resistance. It is a protective tree species with considerable development potential and plays a very important role in environmental management and saline-alkali soil improvement. [0003] In recent decades, due to various reasons such as reclaiming land from the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 侯金艳吴丽芳
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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