A kind of live broadcast method of Dendrobium officinale seed embryo

Abstract

The invention discloses a direct seeding method for a dendrobium candidum embryo. The method comprises the following steps of selecting of direct seeding seeds, preparing of a symbiotic bacteria culture solution, selecting and treating of direct seeding mediums, pre-processing and seeding of the direct seeding seeds and seeding final-period managing. The direct seeding mediums and the direct seeding seeds are treated by adopting the symbiotic bacteria culture solution which is cultivated by manpower, and finally dendrobium candidum seedlings are obtained by the tissue culture. The problems that the germination condition of the dendrobium candidum direct seeding is restricted and the germination rate is very low are solved, a novel approach is provided for manpower cultivating of the dendrobium candidum. The method is simple and practicable, stable and reliable, strong in operability, and low in cost. The germination rate of the dendrobium candidum embryo subjected to the direct seeding by the method can be reached above 86.2%, the transplanting survival rate of the dendrobium candidum with the plant height more than 3 cm can be reached above 97.9%, the cost investment of farmer households can be maximally lowered, the requirement for large-scaled planting of the dendrobium candidum can be met, the industrial production requirement of the dendrobium candidum can be met, and the very important significance is provided for sustainable development of the dendrobium candidum industry.

Description

technical field [0001] The invention belongs to the field of bioengineering, in particular to a direct seeding method of dendrobium officinale seed embryos. Background technique [0002] Dendrobium officinale is a traditional rare and precious Chinese medicinal material, and is listed as "the first of the nine Chinese immortal grasses" in "Tao Zang". Dendrobium officinale has the functions of nourishing yin and clearing heat, promoting body fluid and benefiting stomach, improving human immunity, etc., and has high medicinal value. Because of its incomplete seed development and no endosperm, its natural germination requires the help of an appropriate environment and symbiotic bacteria. It is demanding on natural conditions, and the germination rate under natural conditions is extremely low. Now the wild resources of Dendrobium officinale are on the verge of extinction. [0003] At present, the seedlings required for the cultivation of medicinal Dendrobium officinale are cult...

AI Technical Summary

Problems solved by technology

my country's plant tissue culture technology was developed in the 1950s, and the application research in the Dendrobium industry originated in the 1980s. In the past ten years, tissue culture technology has developed rapidly in the breeding of Dendrobium, but at the same time, problems have gradually emerged. , the tissue culture industry has high technical requirements, large investment, harsh conditions, and poor risk controllability, resulting in high prices for seedlings, which are difficult for growers to accept. In addition, blind investment by non-professionals has led to a decrease in the variety and quality of tissue cultured seedlings. The good and the bad are mixed, and the survival rate of planting is not high, which often causes great losses to the majority of growers, seriously affects the enthusiasm of farmers to plant, and hinders the healthy development of the Dendrobium industry